SNRNP200

Entrez Summary: Pre-mRNA splicing is catalyzed by the spliceosome, a complex of specialized RNA and protein subunits that removes introns from a transcribed pre-mRNA segment. The spliceosome consists of small nuclear RNA proteins (snRNPs) U1, U2, U4, U5 and U6, together with approximately 80 conserved proteins. U5 snRNP contains nine specific proteins. This gene encodes one of the U5 snRNP-specific proteins. This protein belongs to the DEXH-box family of putative RNA helicases. It is a core component of U4/U6-U5 snRNPs and appears to catalyze an ATP-dependent unwinding of U4/U6 RNA duplices. Mutations in this gene cause autosomal-dominant retinitis pigmentosa. Alternatively spliced transcript variants encoding different isoforms have been found, but the full-length nature of these variants has not been determined. [provided by RefSeq, Mar 2010].
UniProt Summary: RNA helicase that plays an essential role in pre-mRNA splicing as component of the U5 snRNP and U4/U6-U5 tri-snRNP complexes. Involved in spliceosome assembly, activation and disassembly. Mediates changes in the dynamic network of RNA-RNA interactions in the spliceosome. Catalyzes the ATP-dependent unwinding of U4/U6 RNA duplices, an essential step in the assembly of a catalytically active spliceosome. {ECO:0000269|PubMed:16723661, ECO:0000269|PubMed:23045696, ECO:0000269|PubMed:8670905, ECO:0000269|PubMed:9539711}.

Pfam Domains GO Terms

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
UMK57 1μM R01 exp59	1.7
Hydroxyurea 15μM R06 exp289	1.71
2-Methoxyestradiol 0.2μM R01 exp40	1.73
All-trans-Retinoic-Acid 8μM R07 exp333	1.73
Mubritinib 0.2μM R01 exp48	1.74
Ribavirin 10μM R01 exp52	1.78
LLY-283 2.6μM R06 exp290	1.81
SGC2043 10μM R03 exp152	1.81
Sucrose 20000μM R06 exp298	1.85
A-769662 20μM R07 exp331	1.86
Suberoylanilide-Hydroxamic-Acid 0.02μM R01 exp53	1.92
Tubastatin-A 2.5μM R07 exp417	1.92
NMS-873 0.07μM R07 exp380	1.95
Doxycycline 10μM R00 exp18	1.97
Dorsomorphin 5μM R07 exp357	1.99
Rifampicin 1μM R00 exp31	2.12
ZLN024 50μM R08 exp538	2.16
Colchicine 0.009μM R05 exp215	2.22
Taxol 0.002μM R01 exp54	2.29
BI-2536 0.001μM R01 exp41	2.31
Parthenolide 2.5μM R05 exp238	2.39
Genistein 15μM R07 exp360	2.42
UM131593 0.1μM R01 exp58	2.49
Lapatinib 5μM R01 exp47	3.12

Global Fraction of Cell Lines Where Essential: 739/739

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	1/1
909776.0	1/1
bile duct	28/28
blood	28/28
bone	26/26
breast	33/33
central nervous system	56/56
cervix	4/4
colorectal	17/17
esophagus	13/13
fibroblast	1/1
gastric	16/16
kidney	21/21
liver	20/20
lung	75/75
lymphocyte	16/16
ovary	26/26
pancreas	24/24
peripheral nervous system	16/16
plasma cell	15/15
prostate	1/1
skin	24/24
soft tissue	9/9
thyroid	2/2
upper aerodigestive	22/22
urinary tract	29/29
uterus	5/5

Expression Distribution

spliceosome conformational change to release U4 (or U4atac) and U1 (or U11)
cis assembly of pre-catalytic spliceosome
spliceosomal conformational changes to generate catalytic conformation
post-mRNA release spliceosomal complex
U2-type catalytic step 1 spliceosome
U5 snRNP
U4/U6 x U5 tri-snRNP complex
mRNA cis splicing, via spliceosome
helicase activity
U2-type precatalytic spliceosome
spliceosomal complex assembly
RNA helicase activity
catalytic step 2 spliceosome
spliceosomal complex
osteoblast differentiation
ribonucleoprotein complex assembly
ribonucleoprotein complex subunit organization
ossification
RNA splicing, via transesterification reactions with bulged adenosine as nucleophile
mRNA splicing, via spliceosome
RNA splicing, via transesterification reactions
RNA splicing
ribonucleoprotein complex biogenesis
mRNA processing
mRNA metabolic process
cellular protein-containing complex assembly
RNA processing
identical protein binding
RNA binding
ATP binding
protein-containing complex assembly
RNA metabolic process
protein-containing complex subunit organization
membrane
gene expression