SCAP [The Human Chemical-Genetic Interaction Map Project]

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======= SCAP =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: SCAP
  * **<color #00a2e8>Official Name</color>**: SREBF chaperone
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=22937|22937]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/Q12770|Q12770]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=SCAP&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20SCAP|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/601510|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**: N/A
  * **<color #00a2e8>UniProt Summary</color>**:  Escort protein required for cholesterol as well as lipid homeostasis. Regulates export of the SCAP/SREBF complex from the ER upon low cholesterol. Formation of a ternary complex with INSIG at high sterol concentrations leads to masking of an ER-export signal in SCAP and retention of the complex in the ER. Low sterol concentrations trigger release of INSIG, a conformational change in the SSC domain of SCAP, unmasking of the ER export signal, recruitment into COPII-coated vesicles, transport to the Golgi complex, proteolytic cleavage of SREBF in the Golgi, release of the transcription factor fragment of SREBF from the membrane, its import into the nucleus and up-regulation of LDLR, INSIG1 and the mevalonate pathway (By similarity). {ECO:0000250}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|WD40|
|Patched|
|Sterol-sensing|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|positive regulation of low-density lipoprotein particle receptor biosynthetic process|
|negative regulation of cholesterol biosynthetic process|
|negative regulation of sterol biosynthetic process|
|negative regulation of cholesterol metabolic process|
|SREBP signaling pathway|
|cellular response to sterol depletion|
|regulation of low-density lipoprotein particle receptor biosynthetic process|
|response to sterol depletion|
|positive regulation of receptor biosynthetic process|
|negative regulation of alcohol biosynthetic process|
|sterol binding|
|negative regulation of steroid biosynthetic process|
|regulation of receptor biosynthetic process|
|negative regulation of steroid metabolic process|
|ER-nucleus signaling pathway|
|regulation of cholesterol biosynthetic process|
|regulation of sterol biosynthetic process|
|regulation of fatty acid biosynthetic process|
|negative regulation of lipid biosynthetic process|
|ER to Golgi transport vesicle membrane|
|regulation of cholesterol metabolic process|
|regulation of alcohol biosynthetic process|
|negative regulation of lipid metabolic process|
|regulation of fatty acid metabolic process|
|regulation of steroid biosynthetic process|
|negative regulation of small molecule metabolic process|
|regulation of steroid metabolic process|
|cholesterol metabolic process|
|secondary alcohol metabolic process|
|unfolded protein binding|
|sterol metabolic process|
|regulation of cellular ketone metabolic process|
|regulation of lipid biosynthetic process|
|response to insulin|
|steroid metabolic process|
|protein-containing complex binding|
|aging|
|alcohol metabolic process|
|response to hypoxia|
|response to decreased oxygen levels|
|response to oxygen levels|
|response to peptide hormone|
|regulation of lipid metabolic process|
|regulation of small molecule metabolic process|
|organic hydroxy compound metabolic process|
|response to peptide|
|protein-containing complex|
|Golgi membrane|
|response to hormone|
|endoplasmic reticulum membrane|
|cellular lipid metabolic process|
|Golgi apparatus|
|response to organonitrogen compound|
|endoplasmic reticulum|
|response to nitrogen compound|
|response to abiotic stimulus|
|lipid metabolic process|
|positive regulation of transcription by RNA polymerase II|
|response to endogenous stimulus|
|positive regulation of transcription, DNA-templated|
|negative regulation of biosynthetic process|
|response to oxygen-containing compound|
|positive regulation of nucleic acid-templated transcription|
|positive regulation of RNA biosynthetic process|
|cellular response to stress|
|positive regulation of protein metabolic process|
|positive regulation of RNA metabolic process|
|small molecule metabolic process|
|immune response|
|positive regulation of nucleobase-containing compound metabolic process|
|positive regulation of macromolecule biosynthetic process|
|positive regulation of cellular biosynthetic process|
|positive regulation of gene expression|
|positive regulation of biosynthetic process|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp226|Cerivastatin 0.15μM R05 exp226]]|-5.36|
|[[:results:exp169|BH1 1μM R04 exp169]]|-4.29|
|[[:results:exp164|Q15 1 to 2μM on day4 R04 exp164]]|-4.21|
|[[:results:exp282|Fluvastatin 2.2μM R06 exp282]]|-4.15|
|[[:results:exp68|Clomiphene 4.4μM R02 exp68]]|-3.93|
|[[:results:exp89|Vemurafenib 6.6μM R02 exp89]]|-3.83|
|[[:results:exp461|BS-181 20μM R08 exp461]]|-3.11|
|[[:results:exp434|Vemurafenib 6.6μM R08 exp434]]|-3.09|
|[[:results:exp454|Bafilomycin-A1 0.009μM R08 exp454]]|-2.89|
|[[:results:exp225|Celastrol 0.12μM R05 exp225]]|-2.77|
|[[:results:exp176|Apcin 50 to 100μM on day4 R04 exp176]]|-2.76|
|[[:results:exp538|ZLN024 50μM R08 exp538]]|-2.74|
|[[:results:exp79|Q15 2.7μM R02 exp79]]|-2.47|
|[[:results:exp131|L-74142 5μM R03 exp131]]|-2.42|
|[[:results:exp84|UM0125461 0.74μM R02 exp84]]|-2.41|
|[[:results:exp341|BRD2 inhibitor II 20μM R07 exp341]]|-2.4|
|[[:results:exp182|IU1-47 25μM R04 exp182]]|-2.39|
|[[:results:exp477|DKK1 89ng/ml R08 exp477]]|-2.25|
|[[:results:exp495|IWR1 50μM R08 exp495]]|-2.24|
|[[:results:exp519|RS-1 10μM R08 exp519]]|-2.19|
|[[:results:exp177|Apcin 25μM plus proTAME 2μM R04 exp177]]|-2.08|
|[[:results:exp97|BI-6727 0.0125μM R03 exp97]]|-2.07|
|[[:results:exp516|Pyrazinamide 100μM R08 exp516]]|-1.92|
|[[:results:exp490|Hydroxychloroquine 30μM R08 exp490]]|-1.91|
|[[:results:exp445|∆-9-Tetrahydrocannabinol 30μM R08 exp445]]|-1.87|
|[[:results:exp344|Chlorpromazine 10μM R07 exp344]]|-1.85|
|[[:results:exp390|Pifithrin-alpha 20μM R07 exp390]]|-1.84|
|[[:results:exp463|Caffeine 2600μM R08 exp463]]|-1.79|
|[[:results:exp508|NN-Dimethylsphingosine 2.5μM R08 exp508]]|-1.79|
|[[:results:exp160|Ribavirin 10 to 15μM on day4 R04 exp160]]|-1.78|
|[[:results:exp537|WNT3A 44ng/ml R08 exp537]]|-1.74|
|[[:results:exp112|S-DABN 8μM R03 exp112]]|-1.73|
|[[:results:exp129|Isonicotinamide 500μM R03 exp129]]|-1.71|
|[[:results:exp25|Oligomycin-A 2μM R00 exp25]]|1.7|
|[[:results:exp448|Ammonium tetrathiomolybdate 10μM R08 exp448]]|1.72|
|[[:results:exp18|Doxycycline 10μM R00 exp18]]|1.74|
|[[:results:exp450|Artemisinin 50μM R08 exp450]]|1.75|
|[[:results:exp439|QNZ 0.01μM R08 exp439]]|1.76|
|[[:results:exp417|Tubastatin-A 2.5μM R07 exp417]]|1.76|
|[[:results:exp12|Chloramphenicol 2μM R00 exp12]]|1.77|
|[[:results:exp349|Cytochalasin-B 5μM R07 exp349]]|1.77|
|[[:results:exp82|Torin1 0.08μM R02 exp82]]|1.79|
|[[:results:exp503|Mitomycin-C 0.06μM R08 exp503]]|1.81|
|[[:results:exp526|Sulforaphane 9μM R08 exp526 no dilution day6]]|1.82|
|[[:results:exp72|LB-100 4.1μM R02 exp72]]|1.85|
|[[:results:exp348|Cyclosporin-A 3μM R07 exp348]]|1.87|
|[[:results:exp512|Olaparib 4μM R08 exp512]]|1.89|
|[[:results:exp333|All-trans-Retinoic-Acid 8μM R07 exp333]]|1.9|
|[[:results:exp343|Centrinone 0.5μM R07 exp343]]|1.9|
|[[:results:exp431|Rotenone 0.07μM R08 exp431]]|1.91|
|[[:results:exp426|FBS-Wisent 0.1 R07 exp426]]|1.92|
|[[:results:exp488|Hippuristanol 0.12μM R08 exp488]]|1.95|
|[[:results:exp447|Amiloride 100μM R08 exp447]]|1.96|
|[[:results:exp455|Benzoate 10000μM R08 exp455]]|1.96|
|[[:results:exp308|Rapamycin 2μM plus FK-506 5μM R07 exp308]]|1.96|
|[[:results:exp441|GSK-J4 1.5μM R08 exp441]]|1.97|
|[[:results:exp539|42°C R08 exp539]]|1.98|
|[[:results:exp38|Wortmannin 5μM R00 exp38]]|1.98|
|[[:results:exp214|2-Deoxy-D-glucose 800μM R05 exp214]]|1.99|
|[[:results:exp289|Hydroxyurea 15μM R06 exp289]]|2|
|[[:results:exp294|Nutlin-3A 1.6μM R06 exp294]]|2.03|
|[[:results:exp320|ABT-702 5μM plus CoCl2 18μM R07 exp320]]|2.04|
|[[:results:exp460|BML-284 0.09μM R08 exp460]]|2.04|
|[[:results:exp134|MS023 2μM R03 exp134]]|2.08|
|[[:results:exp264|Arsenate 40μM R06 exp264]]|2.08|
|[[:results:exp65|Mubritinib 0.2μM R02 exp65]]|2.09|
|[[:results:exp433|LJH685 50μM R08 exp433]]|2.13|
|[[:results:exp216|Erlotinib 10μM R05 exp216]]|2.15|
|[[:results:exp440|Aphidicolin 0.4μM R08 exp440]]|2.19|
|[[:results:exp212|Phenformin 20μM R05 exp212]]|2.24|
|[[:results:exp429|Rapamycin 0.001μM R08 exp429]]|2.24|
|[[:results:exp496|Lead acetate 990μM R08 exp496]]|2.25|
|[[:results:exp456|Benzoate 20000μM R08 exp456]]|2.32|
|[[:results:exp332|Adefovir 20μM R07 exp332]]|2.33|
|[[:results:exp1|5-Fluorouracil 2μM R00 exp1]]|2.34|
|[[:results:exp30|Rapamycin 10μM R00 exp30]]|2.4|
|[[:results:exp359|FK-506 30μM R07 exp359]]|2.42|
|[[:results:exp502|Milciclib 2μM R08 exp502]]|2.46|
|[[:results:exp241|QNZ 0.01μM R05 exp241]]|2.46|
|[[:results:exp6|Bortezomib 0.005μM R00 exp6]]|2.52|
|[[:results:exp485|GSK626616 14μM R08 exp485]]|2.55|
|[[:results:exp525|Sulforaphane 9μM R08 exp525]]|2.59|
|[[:results:exp307|Rapamycin 2μM plus Cyclosporin-A 3μM R07 exp307]]|2.6|
|[[:results:exp524|Staurosporine 0.02μM R08 exp524]]|2.62|
|[[:results:exp489|Hippuristanol 0.12μM R08 exp489 no dilution day6]]|2.75|
|[[:results:exp29|Rapamycin 1μM R00 exp29]]|2.81|
|[[:results:exp418|Tunicamycin 0.04μM R07 exp418]]|2.91|
|[[:results:exp468|CB-5083 0.4μM R08 exp468]]|3|
|[[:results:exp83|Trametinib 10μM R02 exp83]]|3.05|
|[[:results:exp352|Dexamethasone 0.006 to 0.015μM on day4 R07 exp352]]|3.1|
|[[:results:exp346|CoCl2 18μM R07 exp346]]|3.17|
|[[:results:exp329|Hydroxyurea 100μM R07 exp329]]|3.24|
|[[:results:exp229|Dimethyloxaloylglycine 100μM R05 exp229]]|3.29|
|[[:results:exp351|Dexamethasone 0.006μM R07 exp351]]|3.32|
|[[:results:exp191|Hypoxia 5%O2 R04 exp191]]|3.43|
|[[:results:exp420|Tunicamycin 0.04 to 0.125μM  on day4 R07 exp420]]|3.81|
|[[:results:exp335|Aminopterin 0.005μM R07 exp335]]|3.87|
|[[:results:exp407|Thapsigargin 0.005μM R07 exp407]]|3.9|
|[[:results:exp342|Calcium Ionophore 0.4μM R07 exp342]]|4.06|
|[[:results:exp419|Tunicamycin 0.04 to 0.075μM  on day4 R07 exp419]]|4.12|
|[[:results:exp497|Lead acetate 2000μM R08 exp497]]|7.16|
|[[:results:exp498|Lead acetate 2000μM R08 exp498 no dilution day6]]|7.22|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:m:mbtps1|MBTPS1]]|0.671|
|[[:human genes:u:unc50|UNC50]]|0.549|
|[[:human genes:k:kiaa1432|KIAA1432]]|0.543|
|[[:human genes:r:rgp1|RGP1]]|0.511|
|[[:human genes:c:c12orf49|C12orf49]]|0.511|
|[[:human genes:r:ralgapb|RALGAPB]]|0.505|
|[[:human genes:m:mbtps2|MBTPS2]]|0.472|
|[[:human genes:v:vkorc1l1|VKORC1L1]]|0.467|
|[[:human genes:c:cog5|COG5]]|0.461|
|[[:human genes:a:atp6v1g1|ATP6V1G1]]|0.459|
|[[:human genes:c:cog7|COG7]]|0.458|
|[[:human genes:s:srebf2|SREBF2]]|0.448|
|[[:human genes:g:get4|GET4]]|0.447|
|[[:human genes:t:tbc1d23|TBC1D23]]|0.445|
|[[:human genes:c:cept1|CEPT1]]|0.444|
|[[:human genes:h:htt|HTT]]|0.443|
|[[:human genes:c:cog2|COG2]]|0.428|
|[[:human genes:c:chka|CHKA]]|0.427|
|[[:human genes:v:vapa|VAPA]]|0.421|
|[[:human genes:w:wrb|WRB]]|0.42|
|[[:human genes:s:slc25a1|SLC25A1]]|0.419|
|[[:human genes:s:sptlc2|SPTLC2]]|0.413|
|[[:human genes:p:pcyt1a|PCYT1A]]|0.413|
|[[:human genes:g:gpr107|GPR107]]|0.408|
|[[:human genes:r:rab1b|RAB1B]]|0.408|
|[[:human genes:n:n6amt1|N6AMT1]]|0.402|
|[[:human genes:g:gpr89a|GPR89A]]|0.401|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 452/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|19/28|
|blood|24/28|
|bone|10/26|
|breast|17/33|
|central nervous system|30/56|
|cervix|2/4|
|colorectal|14/17|
|esophagus|10/13|
|fibroblast|1/1|
|gastric|9/16|
|kidney|13/21|
|liver|10/20|
|lung|50/75|
|lymphocyte|9/16|
|ovary|11/26|
|pancreas|20/24|
|peripheral nervous system|1/16|
|plasma cell|9/15|
|prostate|1/1|
|skin|13/24|
|soft tissue|5/9|
|thyroid|2/2|
|upper aerodigestive|19/22|
|urinary tract|24/29|
|uterus|3/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 2293
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 7.11 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='SCAP Expression in NALM6 Cells: 7.11'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>