SCAP
Gene Information
- Official Symbol: SCAP
- Official Name: SREBF chaperone
- Aliases and Previous Symbols: N/A
- Entrez ID: 22937
- UniProt: Q12770
- Interactions: BioGRID
- PubMed articles: Open PubMed
- OMIM: Open OMIM
Function Summary
- Entrez Summary: N/A
- UniProt Summary: Escort protein required for cholesterol as well as lipid homeostasis. Regulates export of the SCAP/SREBF complex from the ER upon low cholesterol. Formation of a ternary complex with INSIG at high sterol concentrations leads to masking of an ER-export signal in SCAP and retention of the complex in the ER. Low sterol concentrations trigger release of INSIG, a conformational change in the SSC domain of SCAP, unmasking of the ER export signal, recruitment into COPII-coated vesicles, transport to the Golgi complex, proteolytic cleavage of SREBF in the Golgi, release of the transcription factor fragment of SREBF from the membrane, its import into the nucleus and up-regulation of LDLR, INSIG1 and the mevalonate pathway (By similarity). {ECO:0000250}.
Pfam Domains GO Terms
Pfam Domains
| WD40 |
| Patched |
| Sterol-sensing |
GO Terms
| positive regulation of low-density lipoprotein particle receptor biosynthetic process |
| negative regulation of cholesterol biosynthetic process |
| negative regulation of sterol biosynthetic process |
| negative regulation of cholesterol metabolic process |
| SREBP signaling pathway |
| cellular response to sterol depletion |
| regulation of low-density lipoprotein particle receptor biosynthetic process |
| response to sterol depletion |
| positive regulation of receptor biosynthetic process |
| negative regulation of alcohol biosynthetic process |
| sterol binding |
| negative regulation of steroid biosynthetic process |
| regulation of receptor biosynthetic process |
| negative regulation of steroid metabolic process |
| ER-nucleus signaling pathway |
| regulation of cholesterol biosynthetic process |
| regulation of sterol biosynthetic process |
| regulation of fatty acid biosynthetic process |
| negative regulation of lipid biosynthetic process |
| ER to Golgi transport vesicle membrane |
| regulation of cholesterol metabolic process |
| regulation of alcohol biosynthetic process |
| negative regulation of lipid metabolic process |
| regulation of fatty acid metabolic process |
| regulation of steroid biosynthetic process |
| negative regulation of small molecule metabolic process |
| regulation of steroid metabolic process |
| cholesterol metabolic process |
| secondary alcohol metabolic process |
| unfolded protein binding |
| sterol metabolic process |
| regulation of cellular ketone metabolic process |
| regulation of lipid biosynthetic process |
| response to insulin |
| steroid metabolic process |
| protein-containing complex binding |
| aging |
| alcohol metabolic process |
| response to hypoxia |
| response to decreased oxygen levels |
| response to oxygen levels |
| response to peptide hormone |
| regulation of lipid metabolic process |
| regulation of small molecule metabolic process |
| organic hydroxy compound metabolic process |
| response to peptide |
| protein-containing complex |
| Golgi membrane |
| response to hormone |
| endoplasmic reticulum membrane |
| cellular lipid metabolic process |
| Golgi apparatus |
| response to organonitrogen compound |
| endoplasmic reticulum |
| response to nitrogen compound |
| response to abiotic stimulus |
| lipid metabolic process |
| positive regulation of transcription by RNA polymerase II |
| response to endogenous stimulus |
| positive regulation of transcription, DNA-templated |
| negative regulation of biosynthetic process |
| response to oxygen-containing compound |
| positive regulation of nucleic acid-templated transcription |
| positive regulation of RNA biosynthetic process |
| cellular response to stress |
| positive regulation of protein metabolic process |
| positive regulation of RNA metabolic process |
| small molecule metabolic process |
| immune response |
| positive regulation of nucleobase-containing compound metabolic process |
| positive regulation of macromolecule biosynthetic process |
| positive regulation of cellular biosynthetic process |
| positive regulation of gene expression |
| positive regulation of biosynthetic process |
CRISPR Data
Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)
Compound Hit
Most Correlated Genes in Chemogenomics
| Gene | Correlation |
|---|---|
| MBTPS1 | 0.671 |
| UNC50 | 0.549 |
| KIAA1432 | 0.543 |
| RGP1 | 0.511 |
| C12orf49 | 0.511 |
| RALGAPB | 0.505 |
| MBTPS2 | 0.472 |
| VKORC1L1 | 0.467 |
| COG5 | 0.461 |
| ATP6V1G1 | 0.459 |
| COG7 | 0.458 |
| SREBF2 | 0.448 |
| GET4 | 0.447 |
| TBC1D23 | 0.445 |
| CEPT1 | 0.444 |
| HTT | 0.443 |
| COG2 | 0.428 |
| CHKA | 0.427 |
| VAPA | 0.421 |
| WRB | 0.42 |
| SLC25A1 | 0.419 |
| SPTLC2 | 0.413 |
| PCYT1A | 0.413 |
| GPR107 | 0.408 |
| RAB1B | 0.408 |
| N6AMT1 | 0.402 |
| GPR89A | 0.401 |
Tissues where Essential in the Avana Dataset (DepMap 20Q1)
Global Fraction of Cell Lines Where Essential: 452/739
| Tissue | Fraction Of Cell Lines Where Essential |
|---|---|
| 1290807.0 | 0/1 |
| 909776.0 | 0/1 |
| bile duct | 19/28 |
| blood | 24/28 |
| bone | 10/26 |
| breast | 17/33 |
| central nervous system | 30/56 |
| cervix | 2/4 |
| colorectal | 14/17 |
| esophagus | 10/13 |
| fibroblast | 1/1 |
| gastric | 9/16 |
| kidney | 13/21 |
| liver | 10/20 |
| lung | 50/75 |
| lymphocyte | 9/16 |
| ovary | 11/26 |
| pancreas | 20/24 |
| peripheral nervous system | 1/16 |
| plasma cell | 9/15 |
| prostate | 1/1 |
| skin | 13/24 |
| soft tissue | 5/9 |
| thyroid | 2/2 |
| upper aerodigestive | 19/22 |
| urinary tract | 24/29 |
| uterus | 3/5 |
Essentiality in NALM6
- Essentiality Rank: 2293
- Expression level (log2 read counts): 7.11
Expression Distribution
SCAP Expression in NALM6 Cells: 7.11
