Hydroxyurea 100μM R07 exp329

Inhibits ribonucleotide reductase, scavenges tyrosyl radicals

Class / Subclass 1: Metabolism / Metabolic Enzyme Inhibitor
Class / Subclass 2: DNA Damage, Repair and Replication / Replication Inhibitor

Compound References

PubChem Name: Hydroxyurea
Synonyms: Hydroxycarbamide
CAS #: 127-07-1
PubChem CID: 3657
IUPAC: hydroxyurea
INCHI Name: InChI=1S/CH4N2O2/c2-1(4)3-5/h5H,(H3,2,3,4)
INCHI Key: VSNHCAURESNICA-UHFFFAOYSA-N
Molecular Weight: 76.055
Canonical SMILES: C(=O)(N)NO
Isomeric SMILES: N/A
Molecular Formula: CH4N2O2

Compound Supplier

Supplier Name: Sigma-Aldrich
Catalog #: H8627
Lot #: MKBK3764V

Compound Characterization

HRMS (ESI-TOF) m/z: (M+H)+ Calcd for CH4N2O2 77.03455; found 77.03325

Dose Response Curve

Platform ID: Hydroxyurea
Min: -9.2854; Max: 77.4839

IC	Concentration (µM)
IC10	N/A
IC20	0.0627
IC30	0.0980
IC40	0.1503
IC50	0.2373
IC60	N/A
IC70	N/A
IC80	N/A
IC90	N/A

Screen Summary

Round: 07
Dose: 100µM
Days of incubation: 8
Doublings: 3.2
Numbers of reads: 16504567

Sensitive/Resistant hits (FDR<0.05)	CRANKS	Score Plot	Top 30 Genes	Screen Similarity	Top 30 Sensitive GO terms	Top 30 Resistant GO terms
24/95	Scores	View	View	View	View	View

Gene	CRANKS Score	FDR
RBM26	-4.36	<0.01
GTPBP2	-3.78	<0.01
CFLAR	-3.66	<0.01
KPNA4	-3.45	<0.01
MSI2	-3.30	<0.01
CCDC111	-3.26	<0.01
RRM2	-3.20	0.07
SPR	-3.14	<0.01
VPS25	-3.03	0.05
ZBTB10	-3.01	<0.01
NPRL2	-2.95	<0.01
ATP6V0A2	-2.87	0.01
RECQL5	-2.83	0.05
TRAF2	-2.76	0.02
WDR26	-2.63	0.05
IREB2	-2.60	0.02
KAT7	-2.58	0.02
PDS5B	-2.56	0.02
UBE3D	-2.54	0.02
NPRL3	-2.54	0.06
PPIL1	-2.43	0.04
PLEKHG4B	-2.41	0.04
SSU72	-2.38	0.04
RIF1	-2.37	0.06
WDR33	-2.35	0.17
NDNL2	-2.35	0.10
WEE1	-2.34	0.17
ZBTB7A	-2.33	0.05
TOP1	-2.32	0.05
XPR1	-2.32	0.05
MRPS35	2.57	0.03
MRPL17	2.59	0.04
MRPS22	2.62	<0.01
GFM2	2.62	<0.01
MRPL21	2.63	<0.01
SUPV3L1	2.63	0.01
DARS2	2.63	0.02
MTPAP	2.64	<0.01
MRPS18A	2.64	0.01
NDUFB11	2.69	0.01
KLF16	2.72	0.02
FASTKD5	2.78	0.01
MRPL43	2.82	<0.01
NDUFA2	2.82	<0.01
FTSJ2	2.83	<0.01
YARS2	2.83	<0.01
UQCRC1	2.83	<0.01
MBTPS2	2.87	<0.01
MRPS2	2.89	<0.01
MRPS21	2.91	0.01
COX7B	2.98	<0.01
HSD17B10	2.99	<0.01
AASDHPPT	3.01	<0.01
EPC2	3.02	<0.01
EIF4A1	3.04	<0.01
SLFN11	3.08	<0.01
SCAP	3.24	<0.01
FADS2	3.25	<0.01
MRPL53	3.26	<0.01
MRPL23	3.34	<0.01

Screen	Correlation	Plot
Thimerosal 0.85μM R08 exp529	0.171	View
Pterostilbene 16μM R02 exp78	0.14	View
UM0125461 0.74μM R02 exp84	0.13	View
Oligomycin-A 2μM R00 exp25	0.109	View
PU-H71 1μM R08 exp515	0.09	View
Tanespimycin 14μM R08 exp527	0.083	View
TIC10 10μM R08 exp532	0.078	View
Tubacin 1.6μM R07 exp416	0.077	View
Resveratrol 16μM R03 exp147	0.076	View
ONC212 0.15μM R08 exp513	0.076	View
Olaparib 4μM R08 exp512	0.073	View
LLY-283 2.6μM R06 exp290	0.061	View
Quercetin 20μM R08 exp517	0.058	View
iCRT14 30μM R08 exp492	0.057	View
UMK57 1μM R01 exp59	0.055	View
MK-1775 0.32μM R02 exp75	0.053	View
JQ1 0.8μM R08 exp435	0.053	View
Vemurafenib 6.6μM R08 exp434	0.052	View
TRAIL 50ng/ml R00 exp36	0.052	View
Mitomycin-C 0.06μM R08 exp503	0.051	View
Aphidicolin 0.4μM R08 exp440	0.134	View
GSK-J4 1.5μM R08 exp441	0.127	View
Deferoxamine 11μM R07 exp350	0.111	View
Genistein 15μM R07 exp360	0.103	View
Artemisinin 50μM R08 exp450	0.089	View
Diepoxybutane 3μM R07 exp354	0.08	View
CoCl2 18μM R07 exp346	0.079	View
ABT-702 5μM plus Deferoxamine 11μM R07 exp321	0.076	View
HMS-I2 10μM R06 exp288	0.075	View
Atovaquone 15μM R08 exp451	0.074	View
Cryptotanshinone 12μM R05 exp227	0.073	View
CI-1040 9.5μM R08 exp472	0.065	View
BML-284 0.09μM R08 exp460	0.062	View
BI-D1870 3.15μM R02 exp66	0.06	View
Dorsomorphin 5μM R07 exp357	0.06	View
Calcium Ionophore 0.4μM R07 exp342	0.059	View
AICAR 240μM R05 exp211	0.057	View
CR131-b 0.005μM R08 exp474	0.056	View
42°C R08 exp539	0.052	View

GO Term	Fold Change	Genes
GATOR1 complex	571.69	NPRL2,NPRL3
DNA repair	9.71	PRIMPOL,RECQL5,KAT7,PDS5B,RIF1,WDR33,NSMCE3,ZBTB7A
DNA metabolic process	7.19	PRIMPOL,RRM2,RECQL5,KAT7,PDS5B,RIF1,WDR33,NSMCE3,ZBTB7A

GO Term	Fold Change	Genes
mitochondrial translation	61.98	MRPL23,MRPL53,MRPS21,MRPS2,YARS2,MRPL43,MRPS18A,DARS2,MRPL21,GFM2,MRPS22,MRPL17
mitochondrial large ribosomal subunit	60.29	MRPL23,MRPL53,MRPL43,MRPS18A,MRPL21,MRPL17
organellar large ribosomal subunit	60.29	MRPL23,MRPL53,MRPL43,MRPS18A,MRPL21,MRPL17
mitochondrial gene expression	59.64	MRPL23,MRPL53,HSD17B10,MRPS21,MRPS2,YARS2,MRPL43,FASTKD5,MRPS18A,DARS2,SUPV3L1,MRPL21,GFM2,MRPS22,MRPL17
organellar ribosome	56.52	MRPL23,MRPL53,MRPS21,MRPS2,MRPL43,MRPS18A,MRPL21,MRPS22,MRPL17
mitochondrial ribosome	50.24	MRPL23,MRPL53,MRPS21,MRPS2,MRPL43,MRPS18A,MRPL21,MRPL17
mitochondrial protein-containing complex	30.14	MRPL23,MRPL53,HSD17B10,COX7B,MRPS21,MRPS2,UQCRC1,NDUFA2,MRPL43,NDUFB11,MRPS18A,SUPV3L1,MRPL21,MRPS22,MRPL17
ribosomal subunit	28.58	MRPL23,MRPL53,MRPS21,MRPS2,MRPL43,MRPS18A,MRPL21,MRPS22,MRPL17
ribosome	22.82	MRPL23,MRPL53,MRPS21,MRPS2,MRPL43,MRPS18A,MRPL21,MRPS22,MRPL17
translation	19.96	MRPL23,MRPL53,EIF4A1,MRPS21,MRPS2,YARS2,MRPL43,MRPS18A,DARS2,MRPL21,GFM2,MRPS22,MRPL17
peptide biosynthetic process	18.66	MRPL23,MRPL53,EIF4A1,MRPS21,MRPS2,YARS2,MRPL43,MRPS18A,DARS2,MRPL21,GFM2,MRPS22,MRPL17
mitochondrial matrix	18.62	MRPL23,MRPL53,HSD17B10,MRPS21,MRPS2,YARS2,MRM2,MRPL43,FASTKD5,MRPS18A,DARS2,SUPV3L1,MRPL21,GFM2,MRPS22,MRPL17
mitochondrial inner membrane	15.65	MRPL23,MRPL53,COX7B,MRPS21,MRPS2,UQCRC1,NDUFA2,MRPL43,NDUFB11,MRPS18A,MRPL21,MRPS22,MRPL17
peptide metabolic process	15.06	MRPL23,MRPL53,EIF4A1,MRPS21,MRPS2,YARS2,MRPL43,MRPS18A,DARS2,MRPL21,GFM2,MRPS22,MRPL17
amide biosynthetic process	14.20	MRPL23,MRPL53,EIF4A1,MRPS21,MRPS2,YARS2,MRPL43,MRPS18A,DARS2,MRPL21,GFM2,MRPS22,MRPL17
organelle inner membrane	14.06	MRPL23,MRPL53,COX7B,MRPS21,MRPS2,UQCRC1,NDUFA2,MRPL43,NDUFB11,MRPS18A,MRPL21,MRPS22,MRPL17
amide metabolic process	10.18	MRPL23,MRPL53,EIF4A1,AASDHPPT,MRPS21,MRPS2,YARS2,MRPL43,MRPS18A,DARS2,MRPL21,GFM2,MRPS22,MRPL17
mitochondrial membrane	10.18	MRPL23,MRPL53,COX7B,MRPS21,MRPS2,UQCRC1,NDUFA2,MRPL43,NDUFB11,MRPS18A,MRPL21,MRPS22,MRPL17
mitochondrial envelope	9.55	MRPL23,MRPL53,COX7B,MRPS21,MRPS2,UQCRC1,NDUFA2,MRPL43,NDUFB11,MRPS18A,MRPL21,MRPS22,MRPL17

Hydroxyurea 100μM R07 exp329

Mechanism of Action

Technical Notes

Compound References

Compound Supplier

Compound Characterization

Dose Response Curve

Screen Summary

Screen Results