Caffeine 2600μM R08 exp463 [The Human Chemical-Genetic Interaction Map Project]

CNS stimulant, antagonist of adenosine receptors, inhibits phosphodiesterases, modulates intracellular calcium

Class / Subclass 1: Signal Transduction / GPCR Antagonist
Class / Subclass 2: Environmental Stresses / Food Additive

Compound References

PubChem Name: Caffeine
Synonyms: N/A
CAS #: 58-08-2
PubChem CID: 2519
IUPAC: 1,3,7-trimethylpurine-2,6-dione
INCHI Name: InChI=1S/C8H10N4O2/c1-10-4-9-6-5(10)7(13)12(3)8(14)11(6)2/h4H,1-3H3
INCHI Key: RYYVLZVUVIJVGH-UHFFFAOYSA-N
Molecular Weight: 194.19
Canonical SMILES: CN1C=NC2=C1C(=O)N(C(=O)N2C)C
Isomeric SMILES: N/A
Molecular Formula: C8H10N4O2

Compound Supplier

Supplier Name: Combi-Blocks
Catalog #: QE-5365
Lot #: A39397

Compound Characterization

HRMS (ESI-TOF) m/z: (M+H)+ Calcd for C8H10N4O2 195.08765; found 195.08772

Dose Response Curve

Platform ID: Caffeine
Min: 2.4949; Max: 86.2839

IC	Concentration (µM)
IC10	1086.0000
IC20	1832.0000
IC30	2593.0000
IC40	3448.0000
IC50	4478.0000
IC60	5817.0000
IC70	7734.0000
IC80	10950.0000
IC90	N/A

Screen Summary

Round: 08
Dose: 2600µM
Days of incubation: 8
Doublings: 5.0
Numbers of reads: 14919705

Sensitive/Resistant hits (FDR<0.05)	CRANKS	Score Plot	Top 30 Genes	Screen Similarity	Top 30 Sensitive GO terms	Top 30 Resistant GO terms
9/0	Scores	View	View	View	View	View

Gene	CRANKS Score	FDR
SLC39A9	-4.49	<0.01
GPR107	-4.18	<0.01
ARL1	-3.37	<0.01
MAPK14	-2.97	<0.01
CHKA	-2.81	<0.01
CNOT8	-2.80	<0.01
DHX36	-2.73	0.03
KDSR	-2.61	0.03
NPTN	-2.51	0.04
HIST1H4C	-2.43	0.06
TLK2	-2.42	0.06
ACBD3	-2.32	0.22
FKBP15	-2.23	0.20
MID1IP1	-2.20	0.15
APBA3	-2.20	0.15
EIF2A	-2.20	0.15
PCYT1A	-2.19	0.15
MTA2	-2.18	0.15
NBPF12	-2.16	0.68
GEMIN2	-2.15	0.24
TXNRD1	-2.13	0.46
ANKRD53	-2.13	0.19
UBE2D3	-2.12	0.19
HNRNPA2B1	-2.12	0.34
SMG8	-2.10	0.20
UXS1	-2.08	0.21
SNF8	-2.06	0.30
SIN3B	-2.06	0.22
RALGAPB	-2.05	0.22
RPL8	-2.04	0.71
BAGE4	2.00	0.74
ATPIF1	2.00	0.43
BAGE5	2.00	0.74
BAGE2	2.00	0.74
BAGE3	2.00	0.74
BAGE	2.00	0.74
TBC1D9B	2.01	0.43
ETFB	2.02	0.36
XRCC4	2.03	0.36
SMARCAD1	2.03	0.36
UBXN7	2.05	0.36
BGLAP	2.08	0.36
POLR1E	2.10	0.37
MRPL11	2.10	0.45
TFDP1	2.12	0.37
TVP23C	2.13	0.36
TEPP	2.14	0.36
DPRX	2.14	0.36
ZNF217	2.16	0.36
ABHD5	2.17	0.36
KREMEN1	2.18	0.36
CEBPG	2.18	0.36
EIF3D	2.19	0.43
HSPE1	2.21	0.73
TSNARE1	2.24	0.36
ZNF470	2.25	0.36
PNMAL2	2.32	0.36
SORBS2	2.33	0.36
SLC39A10	2.73	0.10
UBA1	3.00	0.36

Last modified: 2026/01/15 21:36
by 127.0.0.1