SUPT16H

Gene Information

Official Symbol: SUPT16H
Official Name: SPT16 homolog, facilitates chromatin remodeling subunit
Aliases and Previous Symbols: N/A
Entrez ID: 11198
UniProt: Q9Y5B9
Interactions: BioGRID
PubMed articles: Open PubMed
OMIM: Open OMIM

Function Summary

Entrez Summary: Transcription of protein-coding genes can be reconstituted on naked DNA with only the general transcription factors and RNA polymerase II. However, this minimal system cannot transcribe DNA packaged into chromatin, indicating that accessory factors may facilitate access to DNA. One such factor, FACT (facilitates chromatin transcription), interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT is composed of an 80 kDa subunit and a 140 kDa subunit; this gene encodes the 140 kDa subunit. [provided by RefSeq, Feb 2009].
UniProt Summary: Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II). {ECO:0000269|PubMed:10912001, ECO:0000269|PubMed:11239457, ECO:0000269|PubMed:12934006, ECO:0000269|PubMed:16713563, ECO:0000269|PubMed:9489704, ECO:0000269|PubMed:9836642}.

Pfam Domains GO Terms

CRISPR Data

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Global Fraction of Cell Lines Where Essential: 443/726

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	1/1
909776.0	0/1
bile duct	18/28
blood	24/28
bone	18/25
breast	19/33
central nervous system	45/56
cervix	3/4
colorectal	10/17
esophagus	5/13
fibroblast	0/1
gastric	8/15
kidney	21/21
liver	9/20
lung	33/75
lymphocyte	9/14
ovary	18/26
pancreas	11/24
peripheral nervous system	6/16
plasma cell	9/15
prostate	1/1
skin	20/24
soft tissue	4/7
thyroid	0/2
upper aerodigestive	9/22
urinary tract	18/29
uterus	4/5

Essentiality in NALM6

Essentiality Rank: 1425
Expression level (log2 read counts): 9.3

Expression Distribution

Peptidase M24
SPT16
Rtt106

FACT complex
positive regulation of transcription elongation from RNA polymerase II promoter
nucleosome disassembly
chromatin disassembly
protein-DNA complex disassembly
nucleosome binding
regulation of transcription elongation from RNA polymerase II promoter
positive regulation of DNA-templated transcription, elongation
DNA replication-independent nucleosome organization
regulation of DNA-templated transcription, elongation
transcription elongation from RNA polymerase II promoter
DNA-templated transcription, elongation
chromosome
histone binding
chromatin assembly or disassembly
nucleosome organization
regulation of signal transduction by p53 class mediator
DNA replication
protein-containing complex disassembly
protein-DNA complex subunit organization
cellular component disassembly
transcription by RNA polymerase II
DNA repair
transcription, DNA-templated
nucleic acid-templated transcription
RNA biosynthetic process
chromatin organization
DNA metabolic process
cellular response to DNA damage stimulus
chromosome organization
nucleobase-containing compound biosynthetic process
heterocycle biosynthetic process
aromatic compound biosynthetic process
positive regulation of transcription by RNA polymerase II
organic cyclic compound biosynthetic process
RNA binding
positive regulation of transcription, DNA-templated
cellular nitrogen compound biosynthetic process
positive regulation of nucleic acid-templated transcription
positive regulation of RNA biosynthetic process