TDG

Gene Information

Official Symbol: TDG
Official Name: thymine DNA glycosylase
Aliases and Previous Symbols: N/A
Entrez ID: 6996
UniProt: Q13569
Interactions: BioGRID
PubMed articles: Open PubMed
OMIM: Open OMIM

Function Summary

Entrez Summary: N/A
UniProt Summary: DNA glycosylase that plays a key role in active DNA demethylation: specifically recognizes and binds 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC) in the context of CpG sites and mediates their excision through base-excision repair (BER) to install an unmethylated cytosine. Cannot remove 5- hydroxymethylcytosine (5hmC). According to an alternative model, involved in DNA demethylation by mediating DNA glycolase activity toward 5-hydroxymethyluracil (5hmU) produced by deamination of 5hmC. Also involved in DNA repair by acting as a thymine-DNA glycosylase that mediates correction of G/T mispairs to G/C pairs: in the DNA of higher eukaryotes, hydrolytic deamination of 5- methylcytosine to thymine leads to the formation of G/T mismatches. Its role in the repair of canonical base damage is however minor compared to its role in DNA demethylation. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar- phosphate backbone of the DNA and a mispaired thymine. In addition to the G/T, it can remove thymine also from C/T and T/T mispairs in the order G/T » C/T > T/T. It has no detectable activity on apyrimidinic sites and does not catalyze the removal of thymine from A/T pairs or from single-stranded DNA. It can also remove uracil and 5-bromouracil from mispairs with guanine. {ECO:0000269|PubMed:21862836, ECO:0000269|PubMed:22327402, ECO:0000269|PubMed:22573813, ECO:0000269|PubMed:22962365, ECO:0000269|PubMed:8127859, ECO:0000269|PubMed:8407958, ECO:0000269|PubMed:8662714}.

Pfam Domains GO Terms

CRISPR Data

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Global Fraction of Cell Lines Where Essential: 0/726

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	0/1
909776.0	0/1
bile duct	0/28
blood	0/28
bone	0/25
breast	0/33
central nervous system	0/56
cervix	0/4
colorectal	0/17
esophagus	0/13
fibroblast	0/1
gastric	0/15
kidney	0/21
liver	0/20
lung	0/75
lymphocyte	0/14
ovary	0/26
pancreas	0/24
peripheral nervous system	0/16
plasma cell	0/15
prostate	0/1
skin	0/24
soft tissue	0/7
thyroid	0/2
upper aerodigestive	0/22
urinary tract	0/29
uterus	0/5

Essentiality in NALM6

Essentiality Rank: 13212
Expression level (log2 read counts): 7.08

Expression Distribution

G/U mismatch-specific uracil-DNA glycosylase activity
regulation of DNA N-glycosylase activity
pyrimidine-specific mismatch base pair DNA N-glycosylase activity
uracil DNA N-glycosylase activity
oxidative DNA demethylation
depyrimidination
sodium ion binding
mismatched DNA binding
negative regulation of chromatin binding
DNA N-glycosylase activity
base-excision repair, AP site formation
SUMO binding
pyrimidine deoxyribonucleotide catabolic process
chloride ion binding
pyrimidine nucleotide catabolic process
DNA demethylation
deoxyribonucleotide catabolic process
oxidative demethylation
pyrimidine deoxyribonucleotide metabolic process
deoxyribose phosphate catabolic process
regulation of chromatin binding
DNA dealkylation
2-deoxyribonucleotide metabolic process
deoxyribose phosphate metabolic process
mismatch repair
deoxyribonucleotide metabolic process
base-excision repair
pyrimidine-containing compound catabolic process
protein kinase C binding
protein self-association
damaged DNA binding
pyrimidine nucleotide metabolic process
demethylation
DNA methylation or demethylation
nucleotide catabolic process
nucleoside phosphate catabolic process
transcription coregulator activity
DNA modification
PML body
negative regulation of protein binding