TPX2 [The Human Chemical-Genetic Interaction Map Project]

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======= TPX2 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: TPX2
  * **<color #00a2e8>Official Name</color>**: TPX2 microtubule nucleation factor
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=22974|22974]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/Q9ULW0|Q9ULW0]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=TPX2&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20TPX2|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/605917|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**: N/A
  * **<color #00a2e8>UniProt Summary</color>**:  Spindle assembly factor required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules (PubMed:18663142, PubMed:19208764). Activates AURKA by promoting its autophosphorylation at 'Thr-288' and protects this residue against dephosphorylation (PubMed:18663142, PubMed:19208764). TPX2 is inactivated upon binding to importin- alpha (PubMed:26165940). At the onset of mitosis, GOLGA2 interacts with importin-alpha, liberating TPX2 from importin-alpha, allowing TPX2 to activates AURKA kinase and stimulates local microtubule nucleation (PubMed:26165940). {ECO:0000269|PubMed:18663142, ECO:0000269|PubMed:19208764, ECO:0000269|PubMed:26165940}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|TPX2|
|TPX2 importin|
|Aurora-A bind|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|importin-alpha family protein binding|
|axon hillock|
|mitotic spindle assembly|
|regulation of mitotic spindle organization|
|regulation of spindle organization|
|intercellular bridge|
|mitotic spindle organization|
|mitotic spindle|
|spindle assembly|
|microtubule cytoskeleton organization involved in mitosis|
|spindle|
|spindle pole|
|mitotic nuclear division|
|microtubule cytoskeleton|
|spindle organization|
|microtubule organizing center|
|regulation of signal transduction by p53 class mediator|
|regulation of microtubule cytoskeleton organization|
|regulation of G2/M transition of mitotic cell cycle|
|regulation of cell cycle G2/M phase transition|
|regulation of microtubule-based process|
|nuclear division|
|organelle fission|
|microtubule|
|activation of protein kinase activity|
|regulation of mitotic cell cycle phase transition|
|regulation of cell cycle phase transition|
|protein kinase binding|
|microtubule cytoskeleton organization|
|cell division|
|positive regulation of protein kinase activity|
|regulation of cytoskeleton organization|
|positive regulation of kinase activity|
|mitotic cell cycle process|
|regulation of mitotic cell cycle|
|positive regulation of transferase activity|
|microtubule-based process|
|mitotic cell cycle|
|regulation of cell cycle process|
|organelle assembly|
|regulation of protein kinase activity|
|regulation of kinase activity|
|apoptotic process|
|regulation of transferase activity|
|cell cycle process|
|positive regulation of protein phosphorylation|
|programmed cell death|
|positive regulation of phosphorylation|
|cell death|
|cytoskeleton organization|
|positive regulation of phosphorus metabolic process|
|positive regulation of phosphate metabolic process|
|regulation of cell cycle|
|positive regulation of protein modification process|
|regulation of organelle organization|
|cell cycle|
|positive regulation of catalytic activity|
|regulation of protein phosphorylation|
|regulation of phosphorylation|
|positive regulation of cellular protein metabolic process|
|positive regulation of protein metabolic process|
|positive regulation of molecular function|
|regulation of phosphate metabolic process|
|regulation of phosphorus metabolic process|
|regulation of intracellular signal transduction|
|regulation of protein modification process|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp244|SB743921 0.001μM R05 exp244]]|-2.82|
|[[:results:exp125|GSK461364A 0.005μM R03 exp125]]|-2.34|
|[[:results:exp97|BI-6727 0.0125μM R03 exp97]]|-2.31|
|[[:results:exp401|SNS-032 25μM R07 exp401]]|-2.24|
|[[:results:exp518|RK-33 8μM R08 exp518]]|-2.06|
|[[:results:exp90|WYE-354 6μM R02 exp90]]|-2|
|[[:results:exp527|Tanespimycin 14μM R08 exp527]]|-1.92|
|[[:results:exp243|S-trityl-L-cysteine 0.5μM R05 exp243]]|-1.87|
|[[:results:exp529|Thimerosal 0.85μM R08 exp529]]|-1.83|
|[[:results:exp469|CFI-400945 25μM R08 exp469]]|-1.82|
|[[:results:exp468|CB-5083 0.4μM R08 exp468]]|-1.81|
|[[:results:exp486|Heregulin-B 44ng/ml R08 exp486]]|1.71|
|[[:results:exp470|Chloroquine 32μM R08 exp470]]|1.73|
|[[:results:exp375|Lenalidomide 20μM R07 exp375]]|1.73|
|[[:results:exp432|YM155 0.001μM R08 exp432]]|1.75|
|[[:results:exp10|CCCP 0.1μM R00 exp10]]|1.84|
|[[:results:exp5|Alpha-Amanitin 0.5μM R00 exp5]]|1.91|
|[[:results:exp15|Cycloheximide 0.2μM R00 exp15]]|1.91|
|[[:results:exp356|Docosahexaenoic-acid 50μM R07 exp356]]|2.01|
|[[:results:exp457|Bisphenol F 50μM R08 exp457]]|2.03|
|[[:results:exp232|Epothilone-D 0.004 to 0.006μM on day4 R05 exp232]]|2.16|
|[[:results:exp392|PT-1 25μM R07 exp392]]|2.19|
|[[:results:exp11|CCCP 1μM R00 exp11]]|2.2|
|[[:results:exp36|TRAIL 50ng/ml R00 exp36]]|2.2|
|[[:results:exp31|Rifampicin 1μM R00 exp31]]|2.3|
|[[:results:exp152|SGC2043 10μM R03 exp152]]|2.32|
|[[:results:exp16|DABN 2μM R00 exp16]]|2.39|
|[[:results:exp27|Pimelic-diphenylamide-106 0.5μM R00 exp27]]|2.52|
|[[:results:exp495|IWR1 50μM R08 exp495]]|2.56|
|[[:results:exp1|5-Fluorouracil 2μM R00 exp1]]|2.86|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:p:polr2j3|POLR2J3]]|0.554|
|[[:human genes:g:golga6l1|GOLGA6L1]]|0.514|
|[[:human genes:t:tbc1d3h|TBC1D3H]]|0.495|
|[[:human genes:a:aamp|AAMP]]|0.494|
|[[:human genes:t:tbc1d3e|TBC1D3E]]|0.485|
|[[:human genes:t:tbc1d3k|TBC1D3K]]|0.485|
|[[:human genes:t:tbc1d3l|TBC1D3L]]|0.484|
|[[:human genes:t:tbc1d3i|TBC1D3I]]|0.484|
|[[:human genes:t:tbc1d3f|TBC1D3F]]|0.482|
|[[:human genes:p:prim1|PRIM1]]|0.48|
|[[:human genes:t:tceb3c|TCEB3C]]|0.479|
|[[:human genes:t:tbc1d3|TBC1D3]]|0.478|
|[[:human genes:t:tsg101|TSG101]]|0.474|
|[[:human genes:t:tbc1d3g|TBC1D3G]]|0.472|
|[[:human genes:g:golga8o|GOLGA8O]]|0.461|
|[[:human genes:c:cdc73|CDC73]]|0.46|
|[[:human genes:s:skiv2l2|SKIV2L2]]|0.46|
|[[:human genes:t:tbc1d3c|TBC1D3C]]|0.458|
|[[:human genes:c:c14orf93|C14orf93]]|0.458|
|[[:human genes:p:psmb2|PSMB2]]|0.455|
|[[:human genes:t:tceb3cl|TCEB3CL]]|0.452|
|[[:human genes:p:psmb4|PSMB4]]|0.448|
|[[:human genes:l:lrr1|LRR1]]|0.447|
|[[:human genes:c:chaf1a|CHAF1A]]|0.447|
|[[:human genes:s:s1pr4|S1PR4]]|0.446|
|[[:human genes:t:tceb3cl2|TCEB3CL2]]|0.446|
|[[:human genes:c:copg1|COPG1]]|0.445|
|[[:human genes:h:hist1h2be|HIST1H2BE]]|0.445|
|[[:human genes:c:cope|COPE]]|0.444|
|[[:human genes:v:vasn|VASN]]|0.444|
|[[:human genes:r:ranbp2|RANBP2]]|0.443|
|[[:human genes:p:psmd14|PSMD14]]|0.443|
|[[:human genes:p:prpf8|PRPF8]]|0.442|
|[[:human genes:e:egr2|EGR2]]|0.44|
|[[:human genes:s:supt5h|SUPT5H]]|0.439|
|[[:human genes:p:psmd3|PSMD3]]|0.437|
|[[:human genes:n:nup160|NUP160]]|0.435|
|[[:human genes:c:ctr9|CTR9]]|0.435|
|[[:human genes:s:slc4a1|SLC4A1]]|0.435|
|[[:human genes:r:rpl35|RPL35]]|0.429|
|[[:human genes:c:ccna2|CCNA2]]|0.429|
|[[:human genes:r:rrn3|RRN3]]|0.427|
|[[:human genes:p:psmc5|PSMC5]]|0.425|
|[[:human genes:i:ints5|INTS5]]|0.424|
|[[:human genes:c:copa|COPA]]|0.423|
|[[:human genes:g:golga6l3|GOLGA6L3]]|0.421|
|[[:human genes:c:cacna1a|CACNA1A]]|0.419|
|[[:human genes:h:hist2h2bf|HIST2H2BF]]|0.419|
|[[:human genes:n:ndc80|NDC80]]|0.418|
|[[:human genes:r:rrp7a|RRP7A]]|0.418|
|[[:human genes:n:napa|NAPA]]|0.418|
|[[:human genes:c:cwc22|CWC22]]|0.418|
|[[:human genes:r:rplp1|RPLP1]]|0.418|
|[[:human genes:s:snrpa1|SNRPA1]]|0.416|
|[[:human genes:c:ccdc64b|CCDC64B]]|0.414|
|[[:human genes:r:rps2|RPS2]]|0.414|
|[[:human genes:k:krt5|KRT5]]|0.413|
|[[:human genes:u:upf1|UPF1]]|0.413|
|[[:human genes:k:kif3c|KIF3C]]|0.413|
|[[:human genes:p:psmc1|PSMC1]]|0.412|
|[[:human genes:u:utp3|UTP3]]|0.412|
|[[:human genes:s:slc6a17|SLC6A17]]|0.411|
|[[:human genes:s:snip1|SNIP1]]|0.411|
|[[:human genes:p:psap|PSAP]]|0.41|
|[[:human genes:p:psmd7|PSMD7]]|0.41|
|[[:human genes:p:psma6|PSMA6]]|0.41|
|[[:human genes:r:rps5|RPS5]]|0.407|
|[[:human genes:c:ccdc116|CCDC116]]|0.406|
|[[:human genes:r:rps12|RPS12]]|0.405|
|[[:human genes:m:med14|MED14]]|0.404|
|[[:human genes:x:xab2|XAB2]]|0.404|
|[[:human genes:a:atp6v1c1|ATP6V1C1]]|0.404|
|[[:human genes:c:cbwd3|CBWD3]]|0.404|
|[[:human genes:n:narfl|NARFL]]|0.404|
|[[:human genes:l:lad1|LAD1]]|0.403|
|[[:human genes:c:c1qtnf9b|C1QTNF9B]]|0.403|
|[[:human genes:c:col2a1|COL2A1]]|0.403|
|[[:human genes:p:psmb1|PSMB1]]|0.403|
|[[:human genes:g:gar1|GAR1]]|0.401|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 621/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|1/1|
|909776.0|1/1|
|bile duct|26/28|
|blood|25/28|
|bone|25/26|
|breast|26/33|
|central nervous system|45/56|
|cervix|2/4|
|colorectal|15/17|
|esophagus|11/13|
|fibroblast|1/1|
|gastric|14/16|
|kidney|14/21|
|liver|15/20|
|lung|66/75|
|lymphocyte|15/16|
|ovary|24/26|
|pancreas|18/24|
|peripheral nervous system|14/16|
|plasma cell|14/15|
|prostate|1/1|
|skin|18/24|
|soft tissue|8/9|
|thyroid|2/2|
|upper aerodigestive|15/22|
|urinary tract|21/29|
|uterus|3/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 210
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 7.96 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='TPX2 Expression in NALM6 Cells: 7.96'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>