TPX2

Entrez Summary: N/A
UniProt Summary: Spindle assembly factor required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules (PubMed:18663142, PubMed:19208764). Activates AURKA by promoting its autophosphorylation at 'Thr-288' and protects this residue against dephosphorylation (PubMed:18663142, PubMed:19208764). TPX2 is inactivated upon binding to importin- alpha (PubMed:26165940). At the onset of mitosis, GOLGA2 interacts with importin-alpha, liberating TPX2 from importin-alpha, allowing TPX2 to activates AURKA kinase and stimulates local microtubule nucleation (PubMed:26165940). {ECO:0000269|PubMed:18663142, ECO:0000269|PubMed:19208764, ECO:0000269|PubMed:26165940}.

Pfam Domains GO Terms

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
SB743921 0.001μM R05 exp244	-2.82
GSK461364A 0.005μM R03 exp125	-2.34
BI-6727 0.0125μM R03 exp97	-2.31
SNS-032 25μM R07 exp401	-2.24
RK-33 8μM R08 exp518	-2.06
WYE-354 6μM R02 exp90	-2
Tanespimycin 14μM R08 exp527	-1.92
S-trityl-L-cysteine 0.5μM R05 exp243	-1.87
Thimerosal 0.85μM R08 exp529	-1.83
CFI-400945 25μM R08 exp469	-1.82
CB-5083 0.4μM R08 exp468	-1.81
Heregulin-B 44ng/ml R08 exp486	1.71
Chloroquine 32μM R08 exp470	1.73
Lenalidomide 20μM R07 exp375	1.73
YM155 0.001μM R08 exp432	1.75
CCCP 0.1μM R00 exp10	1.84
Alpha-Amanitin 0.5μM R00 exp5	1.91
Cycloheximide 0.2μM R00 exp15	1.91
Docosahexaenoic-acid 50μM R07 exp356	2.01
Bisphenol F 50μM R08 exp457	2.03
Epothilone-D 0.004 to 0.006μM on day4 R05 exp232	2.16
PT-1 25μM R07 exp392	2.19
CCCP 1μM R00 exp11	2.2
TRAIL 50ng/ml R00 exp36	2.2
Rifampicin 1μM R00 exp31	2.3
SGC2043 10μM R03 exp152	2.32
DABN 2μM R00 exp16	2.39
Pimelic-diphenylamide-106 0.5μM R00 exp27	2.52
IWR1 50μM R08 exp495	2.56
5-Fluorouracil 2μM R00 exp1	2.86

Global Fraction of Cell Lines Where Essential: 621/739

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	1/1
909776.0	1/1
bile duct	26/28
blood	25/28
bone	25/26
breast	26/33
central nervous system	45/56
cervix	2/4
colorectal	15/17
esophagus	11/13
fibroblast	1/1
gastric	14/16
kidney	14/21
liver	15/20
lung	66/75
lymphocyte	15/16
ovary	24/26
pancreas	18/24
peripheral nervous system	14/16
plasma cell	14/15
prostate	1/1
skin	18/24
soft tissue	8/9
thyroid	2/2
upper aerodigestive	15/22
urinary tract	21/29
uterus	3/5

Expression Distribution

importin-alpha family protein binding
axon hillock
mitotic spindle assembly
regulation of mitotic spindle organization
regulation of spindle organization
intercellular bridge
mitotic spindle organization
mitotic spindle
spindle assembly
microtubule cytoskeleton organization involved in mitosis
spindle
spindle pole
mitotic nuclear division
microtubule cytoskeleton
spindle organization
microtubule organizing center
regulation of signal transduction by p53 class mediator
regulation of microtubule cytoskeleton organization
regulation of G2/M transition of mitotic cell cycle
regulation of cell cycle G2/M phase transition
regulation of microtubule-based process
nuclear division
organelle fission
microtubule
activation of protein kinase activity
regulation of mitotic cell cycle phase transition
regulation of cell cycle phase transition
protein kinase binding
microtubule cytoskeleton organization
cell division
positive regulation of protein kinase activity
regulation of cytoskeleton organization
positive regulation of kinase activity
mitotic cell cycle process
regulation of mitotic cell cycle
positive regulation of transferase activity
microtubule-based process
mitotic cell cycle
regulation of cell cycle process
organelle assembly