CPSF3

Entrez Summary: N/A
UniProt Summary: Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. Has endonuclease activity, and functions as mRNA 3'-end-processing endonuclease. Also involved in the histone 3'-end pre-mRNA processing. U7 snRNP- dependent protein that induces both the 3'-endoribonucleolytic cleavage of histone pre-mRNAs and acts as a 5' to 3' exonuclease for degrading the subsequent downstream cleavage product (DCP) of mature histone mRNAs. Cleavage occurs after the 5'-ACCCA-3' sequence in the histone pre-mRNA leaving a 3'hydroxyl group on the upstream fragment containing the stem loop (SL) and 5' phosphate on the downstream cleavage product (DCP) starting with CU nucleotides. The U7-dependent 5' to 3' exonuclease activity is processive and degrades the DCP RNA substrate even after complete removal of the U7-binding site. Binds to the downstream cleavage product (DCP) of histone pre-mRNAs and the cleaved DCP RNA substrate in a U7 snRNP dependent manner. {ECO:0000269|PubMed:14749727, ECO:0000269|PubMed:15037765, ECO:0000269|PubMed:17128255, ECO:0000269|PubMed:18688255}.

Pfam Domains GO Terms

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Global Fraction of Cell Lines Where Essential: 492/726

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	1/1
909776.0	1/1
bile duct	20/28
blood	18/28
bone	20/25
breast	20/33
central nervous system	43/56
cervix	4/4
colorectal	11/17
esophagus	11/13
fibroblast	1/1
gastric	10/15
kidney	14/21
liver	11/20
lung	52/75
lymphocyte	13/14
ovary	16/26
pancreas	18/24
peripheral nervous system	14/16
plasma cell	12/15
prostate	1/1
skin	13/24
soft tissue	5/7
thyroid	2/2
upper aerodigestive	15/22
urinary tract	17/29
uterus	4/5

Expression Distribution

mRNA 3-end processing by stem-loop binding and cleavage
5-3 exonuclease activity
mRNA cleavage and polyadenylation specificity factor complex
mRNA cleavage
endoribonuclease activity
mRNA polyadenylation
histone mRNA metabolic process
RNA polyadenylation
termination of RNA polymerase II transcription
DNA-templated transcription, termination
RNA phosphodiester bond hydrolysis, endonucleolytic
mRNA 3-end processing
mRNA export from nucleus
mRNA-containing ribonucleoprotein complex export from nucleus
ribonucleoprotein complex export from nucleus
ribonucleoprotein complex localization
RNA export from nucleus
RNA 3-end processing
protein export from nucleus
mRNA transport
nuclear export
RNA phosphodiester bond hydrolysis
nucleic acid transport
RNA transport
establishment of RNA localization
RNA localization
nucleobase-containing compound transport
protein-containing complex localization
nucleocytoplasmic transport
nuclear transport
mRNA splicing, via spliceosome
RNA splicing, via transesterification reactions with bulged adenosine as nucleophile
nucleic acid phosphodiester bond hydrolysis
RNA splicing, via transesterification reactions
RNA splicing
mRNA processing
transcription by RNA polymerase II
transcription, DNA-templated
nucleic acid-templated transcription
RNA biosynthetic process