ERCC3

Gene Information

Official Symbol: ERCC3
Official Name: ERCC excision repair 3, TFIIH core complex helicase subunit
Aliases and Previous Symbols: N/A
Entrez ID: 2071
UniProt: P19447
Interactions: BioGRID
PubMed articles: Open PubMed
OMIM: Open OMIM

Function Summary

Entrez Summary: This gene encodes an ATP-dependent DNA helicase that functions in nucleotide excision repair. The encoded protein is a subunit of basal transcription factor 2 (TFIIH) and, therefore, also functions in class II transcription. Mutations in this gene are associated with Xeroderma pigmentosum B, Cockayne's syndrome, and trichothiodystrophy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]. COMPLETENESS: complete on the 3' end.
UniProt Summary: ATP-dependent 3'-5' DNA helicase, component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. The ATPase activity of XPB/ERCC3, but not its helicase activity, is required for DNA opening. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. The ATP- dependent helicase activity of XPB/ERCC3 is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription. {ECO:0000269|PubMed:10024882, ECO:0000269|PubMed:8157004}.

Pfam Domains GO Terms

CRISPR Data

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
Actinomycin-D 0.001μM R00 exp3	-2.29
NN-Dimethylsphingosine 2.5μM R08 exp508	-1.98
Talazoparib 0.006μM R06 exp299	-1.97
Campthothecin 0.001μM R06 exp270	-1.93
Wortmannin 5μM R00 exp38	-1.86
Citral 50μM R06 exp274	-1.78
Bleomycin 5μM R08 exp459	-1.76
Pimelic-diphenylamide-106 5μM R00 exp28	-1.74
Prochlorperazine 5.2μM R02 exp77	-1.73
TNF-alpha 44ng/ml R08 exp533	1.9
Golgicide-A 4μM R03 exp122	1.92

Global Fraction of Cell Lines Where Essential: 13/726

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	0/1
909776.0	0/1
bile duct	1/28
blood	0/28
bone	2/25
breast	0/33
central nervous system	0/56
cervix	1/4
colorectal	0/17
esophagus	0/13
fibroblast	0/1
gastric	0/15
kidney	0/21
liver	0/20
lung	2/75
lymphocyte	0/14
ovary	0/26
pancreas	0/24
peripheral nervous system	0/16
plasma cell	2/15
prostate	0/1
skin	0/24
soft tissue	1/7
thyroid	0/2
upper aerodigestive	0/22
urinary tract	1/29
uterus	0/5

Essentiality in NALM6

Essentiality Rank: 246
Expression level (log2 read counts): 6.42

Expression Distribution

Helicase C
ResIII

nucleotide-excision repair factor 3 complex
transcriptional preinitiation complex
transcription factor TFIIH core complex
DNA topological change
transcription factor TFIIH holo complex
UV protection
RNA polymerase II CTD heptapeptide repeat kinase activity
3-5 DNA helicase activity
nucleotide-excision repair, preincision complex stabilization
nucleotide-excision repair, DNA incision, 3-to lesion
nucleotide-excision repair, DNA duplex unwinding
global genome nucleotide-excision repair
nucleotide-excision repair, preincision complex assembly
transcription elongation from RNA polymerase I promoter
termination of RNA polymerase I transcription
7-methylguanosine mRNA capping
hair cell differentiation
helicase activity
7-methylguanosine RNA capping
RNA capping
transcription initiation from RNA polymerase I promoter
nucleotide-excision repair, DNA incision, 5-to lesion
transcription factor TFIID complex
transcription by RNA polymerase I
nucleotide-excision repair, DNA incision
DNA-dependent ATPase activity
damaged DNA binding
DNA helicase activity
DNA-templated transcription, termination
transcription-coupled nucleotide-excision repair
transcription elongation from RNA polymerase II promoter
DNA-templated transcription, elongation
protein N-terminus binding
nucleotide-excision repair
DNA duplex unwinding
DNA geometric change
response to UV
transcription initiation from RNA polymerase II promoter
protein C-terminus binding
protein-DNA complex assembly