NELFB

Gene Information

Official Symbol: NELFB
Official Name: negative elongation factor complex member B
Aliases and Previous Symbols: N/A
Entrez ID: 25920
UniProt: Q8WX92
Interactions: BioGRID
PubMed articles: Open PubMed
OMIM: Open OMIM

Function Summary

Entrez Summary: N/A
UniProt Summary: Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II. Binds RNA which may help to stabilize the NELF complex on nucleic acid. In vitro, binds weakly to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1. May be able to induce chromatin unfolding. {ECO:0000269|PubMed:10199401, ECO:0000269|PubMed:12612062, ECO:0000269|PubMed:23884411, ECO:0000269|PubMed:27282391}.

Pfam Domains GO Terms

CRISPR Data

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
JQ1 0.8μM R08 exp435	-3.24
PU-H71 1μM R08 exp515	-2.35
Deferoxamine 11μM R07 exp350	-2.29
Pimelic-diphenylamide-106 5μM R00 exp28	-2.22
BVD-523 15μM R02 exp67	-2.18
BI-D1870 3.15μM R02 exp66	-1.95
Dinaciclib 0.007μM R07 exp355	-1.77
Prochlorperazine 5.2μM R02 exp77	1.74
Deguelin 0.05μM R02 exp69	2.05
Mubritinib 0.2μM R02 exp65	2.18
Trimetrexate 0.03μM R08 exp535	2.76

Global Fraction of Cell Lines Where Essential: 708/726

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	0/1
909776.0	1/1
bile duct	27/28
blood	27/28
bone	25/25
breast	31/33
central nervous system	56/56
cervix	4/4
colorectal	17/17
esophagus	12/13
fibroblast	1/1
gastric	14/15
kidney	20/21
liver	20/20
lung	73/75
lymphocyte	14/14
ovary	26/26
pancreas	24/24
peripheral nervous system	16/16
plasma cell	15/15
prostate	1/1
skin	24/24
soft tissue	6/7
thyroid	2/2
upper aerodigestive	22/22
urinary tract	28/29
uterus	5/5

Essentiality in NALM6

Essentiality Rank: 138
Expression level (log2 read counts): 6.37

Expression Distribution

Gene	Correlation
NELFA	0.508
BRD2	0.473
PDS5B	0.472
NELFCD	0.421
IPPK	0.409

NELF complex
negative regulation of transcription elongation from RNA polymerase II promoter
negative regulation of DNA-templated transcription, elongation
negative regulation of stem cell differentiation
regulation of transcription elongation from RNA polymerase II promoter
positive regulation of viral transcription
regulation of DNA-templated transcription, elongation
regulation of viral transcription
transcription elongation from RNA polymerase II promoter
DNA-templated transcription, elongation
positive regulation of viral process
regulation of stem cell differentiation
stem cell differentiation
regulation of viral process
regulation of symbiosis, encompassing mutualism through parasitism
transcription by RNA polymerase II
positive regulation of multi-organism process
cell population proliferation
transcription, DNA-templated
nucleic acid-templated transcription
RNA biosynthetic process
negative regulation of cell differentiation
regulation of multi-organism process
negative regulation of transcription by RNA polymerase II
negative regulation of developmental process
nucleobase-containing compound biosynthetic process
heterocycle biosynthetic process
aromatic compound biosynthetic process
negative regulation of transcription, DNA-templated
negative regulation of nucleic acid-templated transcription
negative regulation of RNA biosynthetic process
organic cyclic compound biosynthetic process
negative regulation of RNA metabolic process
negative regulation of cellular macromolecule biosynthetic process
RNA binding
negative regulation of nucleobase-containing compound metabolic process
negative regulation of macromolecule biosynthetic process
negative regulation of cellular biosynthetic process
negative regulation of biosynthetic process
cellular nitrogen compound biosynthetic process