BUB1

Gene Information

Official Symbol: BUB1
Official Name: BUB1 mitotic checkpoint serine/threonine kinase
Aliases and Previous Symbols: N/A
Entrez ID: 699
UniProt: O43683
Interactions: BioGRID
PubMed articles: Open PubMed
OMIM: Open OMIM

Function Summary

Entrez Summary: This gene encodes a serine/threonine-protein kinase that play a central role in mitosis. The encoded protein functions in part by phosphorylating members of the mitotic checkpoint complex and activating the spindle checkpoint. This protein also plays a role in inhibiting the activation of the anaphase promoting complex/cyclosome. This protein may also function in the DNA damage response. Mutations in this gene have been associated with aneuploidy and several forms of cancer. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013].
UniProt Summary: Serine/threonine-protein kinase that performs 2 crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. Has a key role in the assembly of checkpoint proteins at the kinetochore, being required for the subsequent localization of CENPF, BUB1B, CENPE and MAD2L1. Required for the kinetochore localization of PLK1. Required for centromeric enrichment of AUKRB in prometaphase. Plays an important role in defining SGO1 localization and thereby affects sister chromatid cohesion. Acts as a substrate for anaphase-promoting complex or cyclosome (APC/C) in complex with its activator CDH1 (APC/C-Cdh1). Necessary for ensuring proper chromosome segregation and binding to BUB3 is essential for this function. Can regulate chromosome segregation in a kinetochore-independent manner. Can phosphorylate BUB3. The BUB1-BUB3 complex plays a role in the inhibition of APC/C when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1. Kinase activity is essential for inhibition of APC/CCDC20 and for chromosome alignment but does not play a major role in the spindle-assembly checkpoint activity. Mediates cell death in response to chromosome missegregation and acts to suppress spontaneous tumorigenesis. {ECO:0000269|PubMed:10198256, ECO:0000269|PubMed:15020684, ECO:0000269|PubMed:15525512, ECO:0000269|PubMed:15723797, ECO:0000269|PubMed:16760428, ECO:0000269|PubMed:17158872, ECO:0000269|PubMed:19487456, ECO:0000269|PubMed:20739936}.

Pfam Domains GO Terms

CRISPR Data

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
BI-6727 0.0125μM R03 exp97	-2.46
BI-2536 0.0042μM R03 exp95	-2.2
GSK591 2.6μM R03 exp128	-1.97
Bisphenol A 100μM R06 exp269	-1.88
Lapatinib 5μM R01 exp47	-1.72
Demecolcine 0.03μM R05 exp228	-1.71
2-Methoxyestradiol 0.55 to 0.75μM on day4 R07 exp311	-1.7
GW501516 25μM R06 exp285	2.01
SB743921 0.001μM R05 exp244	2.15
Rotenone 2μM R00 exp33	2.42

Global Fraction of Cell Lines Where Essential: 23/726

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	0/1
909776.0	0/1
bile duct	0/28
blood	0/28
bone	0/25
breast	0/33
central nervous system	0/56
cervix	0/4
colorectal	0/17
esophagus	1/13
fibroblast	0/1
gastric	1/15
kidney	3/21
liver	1/20
lung	2/75
lymphocyte	0/14
ovary	1/26
pancreas	0/24
peripheral nervous system	1/16
plasma cell	1/15
prostate	0/1
skin	1/24
soft tissue	0/7
thyroid	0/2
upper aerodigestive	1/22
urinary tract	1/29
uterus	0/5

Essentiality in NALM6

Essentiality Rank: 2958
Expression level (log2 read counts): 8.14

Expression Distribution

Gene	Correlation
HARS	0.519
KNTC1	0.442
GAK	0.403

Pkinase Tyr
Mad3 BUB1 I
Pkinase

condensed nuclear chromosome outer kinetochore
meiotic sister chromatid cohesion, centromeric
centromeric sister chromatid cohesion
meiotic sister chromatid cohesion
meiotic sister chromatid segregation
condensed nuclear chromosome kinetochore
meiosis II
meiosis II cell cycle process
regulation of sister chromatid cohesion
mitotic spindle assembly checkpoint
spindle checkpoint
mitotic spindle checkpoint
spindle assembly checkpoint
negative regulation of mitotic metaphase/anaphase transition
negative regulation of metaphase/anaphase transition of cell cycle
negative regulation of mitotic sister chromatid separation
negative regulation of chromosome separation
negative regulation of mitotic sister chromatid segregation
negative regulation of sister chromatid segregation
negative regulation of chromosome segregation
negative regulation of mitotic nuclear division
negative regulation of nuclear division
sister chromatid cohesion
regulation of mitotic metaphase/anaphase transition
regulation of metaphase/anaphase transition of cell cycle
regulation of mitotic sister chromatid separation
regulation of chromosome separation
chromosome organization involved in meiotic cell cycle
regulation of mitotic sister chromatid segregation
regulation of sister chromatid segregation
kinetochore
condensed chromosome kinetochore
meiotic chromosome segregation
regulation of chromosome segregation
negative regulation of chromosome organization
sister chromatid segregation
meiotic nuclear division
mitotic cell cycle checkpoint
meiotic cell cycle process
regulation of mitotic nuclear division