FICD

Entrez Summary: N/A
UniProt Summary: Protein that can both mediate the addition of adenosine 5'-monophosphate (AMP) to specific residues of target proteins (AMPylation), and the removal of the same modification from target proteins (de-AMPylation), depending on the context (By similarity). The side chain of Glu-231 determines which of the two opposing activities (AMPylase or de-AMPylase) will take place (By similarity). Acts as a key regulator of the ERN1/IRE1-mediated unfolded protein response (UPR) by mediating AMPylation or de- AMPylation of HSPA5/BiP (PubMed:25601083). In unstressed cells, acts as an adenylyltransferase by mediating AMPylation of HSPA5/BiP at 'Thr-518', thereby inactivating it (By similarity). In response to endoplasmic reticulum stress, acts as a phosphodiesterase by mediating removal of ATP (de-AMPylation) from HSPA5/BiP at 'Thr-518', leading to restore HSPA5/BiP activity (By similarity). Although it is able to AMPylate RhoA, Rac and Cdc42 Rho GTPases in vitro, Rho GTPases do not constitute physiological substrates (PubMed:19362538, PubMed:25601083). {ECO:0000250|UniProtKB:A0A061I403, ECO:0000269|PubMed:22266942, ECO:0000269|PubMed:25435325, ECO:0000269|PubMed:25601083, ECO:0000305|PubMed:19362538}.

Pfam Domains GO Terms

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Global Fraction of Cell Lines Where Essential: 0/739

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	0/1
909776.0	0/1
bile duct	0/28
blood	0/28
bone	0/26
breast	0/33
central nervous system	0/56
cervix	0/4
colorectal	0/17
esophagus	0/13
fibroblast	0/1
gastric	0/16
kidney	0/21
liver	0/20
lung	0/75
lymphocyte	0/16
ovary	0/26
pancreas	0/24
peripheral nervous system	0/16
plasma cell	0/15
prostate	0/1
skin	0/24
soft tissue	0/9
thyroid	0/2
upper aerodigestive	0/22
urinary tract	0/29
uterus	0/5

Expression Distribution

Screen	Score
ZLN024 50μM R08 exp538	-1.78
ABT-702 5μM plus Dimethyloxaloylglycine 11μM R07 exp319	1.72
NFN1 0.1μM R01 exp49	1.9
Lapatinib 5μM R01 exp47	1.95

protein denucleotidylation
protein adenylylhydrolase activity
protein deadenylylation
protein adenylylation
protein nucleotidylation
protein adenylyltransferase activity
regulation of IRE1-mediated unfolded protein response
regulation of endoplasmic reticulum unfolded protein response
Hsp70 protein binding
negative regulation of GTPase activity
regulation of response to endoplasmic reticulum stress
chaperone binding
integral component of endoplasmic reticulum membrane
response to unfolded protein
response to topologically incorrect protein
response to endoplasmic reticulum stress
negative regulation of hydrolase activity
regulation of GTPase activity
regulation of cellular response to stress
negative regulation of catalytic activity
protein homodimerization activity
identical protein binding
negative regulation of molecular function
regulation of hydrolase activity
regulation of response to stress
ATP binding
cellular response to stress