INSIG1

Gene Information

Official Symbol: INSIG1
Official Name: insulin induced gene 1
Aliases and Previous Symbols: N/A
Entrez ID: 3638
UniProt: O15503
Interactions: BioGRID
PubMed articles: Open PubMed
OMIM: Open OMIM

Function Summary

Entrez Summary: N/A
UniProt Summary: Mediates feedback control of cholesterol synthesis by controlling SCAP and HMGCR. Functions by blocking the processing of sterol regulatory element-binding proteins (SREBPs). Capable of retaining the SCAP-SREBF2 complex in the ER thus preventing it from escorting SREBPs to the Golgi. Initiates the sterol-mediated ubiquitin-mediated endoplasmic reticulum-associated degradation (ERAD) of HMGCR via recruitment of the reductase to the ubiquitin ligase, AMFR/gp78. May play a role in growth and differentiation of tissues involved in metabolic control. May play a regulatory role during G0/G1 transition of cell growth. {ECO:0000269|PubMed:12202038, ECO:0000269|PubMed:12535518, ECO:0000269|PubMed:16168377, ECO:0000269|PubMed:16399501, ECO:0000269|PubMed:16606821}.

Pfam Domains GO Terms

CRISPR Data

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
Tubacin 1.6μM R07 exp416	-1.98
UM0125461 0.74μM R02 exp84	-1.86
Cannabidiol 20μM R08 exp466	1.7
Fluvastatin 2.2μM R06 exp282	2.09
Q15 1 to 2μM on day4 R04 exp164	2.19
Vemurafenib 6.6μM R02 exp89	2.28
Q15 2.7μM R02 exp79	2.98
Cerivastatin 0.15μM R05 exp226	3.06
Vemurafenib 6.6μM R08 exp434	3.08

Global Fraction of Cell Lines Where Essential: 1/739

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	0/1
909776.0	0/1
bile duct	0/28
blood	0/28
bone	0/26
breast	0/33
central nervous system	0/56
cervix	0/4
colorectal	0/17
esophagus	0/13
fibroblast	0/1
gastric	0/16
kidney	0/21
liver	0/20
lung	0/75
lymphocyte	0/16
ovary	0/26
pancreas	0/24
peripheral nervous system	1/16
plasma cell	0/15
prostate	0/1
skin	0/24
soft tissue	0/9
thyroid	0/2
upper aerodigestive	0/22
urinary tract	0/29
uterus	0/5

Essentiality in NALM6

Essentiality Rank: 2625
Expression level (log2 read counts): 7.1

Expression Distribution

negative regulation of cargo loading into COPII-coated vesicle
SREBP-SCAP complex retention in endoplasmic reticulum
regulation of cargo loading into COPII-coated vesicle
negative regulation of SREBP signaling pathway
SREBP-SCAP-Insig complex
regulation of SREBP signaling pathway
SREBP signaling pathway
cellular response to sterol depletion
cranial suture morphogenesis
negative regulation of protein exit from endoplasmic reticulum
response to sterol depletion
maintenance of protein localization in endoplasmic reticulum
negative regulation of fatty acid biosynthetic process
regulation of ER to Golgi vesicle-mediated transport
craniofacial suture morphogenesis
middle ear morphogenesis
cellular response to sterol
negative regulation of steroid biosynthetic process
negative regulation of steroid metabolic process
negative regulation of fatty acid metabolic process
regulation of protein exit from endoplasmic reticulum
response to sterol
ER-nucleus signaling pathway
maintenance of protein localization in organelle
negative regulation of intracellular protein transport
cholesterol biosynthetic process
secondary alcohol biosynthetic process
regulation of fatty acid biosynthetic process
negative regulation of fat cell differentiation
sterol biosynthetic process
negative regulation of lipid biosynthetic process
negative regulation of intracellular transport
cranial skeletal system development
triglyceride metabolic process
maintenance of protein location in cell
cholesterol homeostasis
sterol homeostasis
negative regulation of lipid metabolic process
regulation of fatty acid metabolic process
regulation of steroid biosynthetic process