LIN28B

Gene Information

Official Symbol: LIN28B
Official Name: lin-28 homolog B
Aliases and Previous Symbols: N/A
Entrez ID: 389421
UniProt: Q6ZN17
Interactions: BioGRID
PubMed articles: Open PubMed
OMIM: Open OMIM

Function Summary

Entrez Summary: The protein encoded by this gene belongs to the lin-28 family, which is characterized by the presence of a cold-shock domain and a pair of CCHC zinc finger domains. This gene is highly expressed in testis, fetal liver, placenta, and in primary human tumors and cancer cell lines. It is negatively regulated by microRNAs that target sites in the 3' UTR, and overexpression of this gene in primary tumors is linked to the repression of let-7 family of microRNAs and derepression of let-7 targets, which facilitates cellular transformation. [provided by RefSeq, Jun 2012].
UniProt Summary: Suppressor of microRNA (miRNA) biogenesis, including that of let-7 and possibly of miR107, miR-143 and miR-200c. Binds primary let-7 transcripts (pri-let-7), including pri-let-7g and pri-let-7a-1, and sequester them in the nucleolus, away from the microprocessor complex, hence preventing their processing into mature miRNA (PubMed:22118463). Does not act on pri-miR21 (PubMed:22118463). The repression of let-7 expression is required for normal development and contributes to maintain the pluripotent state of embryonic stem cells by preventing let-7-mediated differentiation. When overexpressed, recruits ZCCHC11/TUT4 uridylyltransferase to pre-let-7 transcripts, leading to their terminal uridylation and degradation (PubMed:19703396). This activity might not be relevant in vivo, as LIN28B-mediated inhibition of let-7 miRNA maturation appears to be ZCCHC11- independent (PubMed:22118463). Interaction with target pre-miRNAs occurs via an 5'-GGAG-3' motif in the pre-miRNA terminal loop. Mediates MYC-induced let-7 repression (By similarity). When overexpressed, isoform 1 stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth. {ECO:0000250|UniProtKB:Q45KJ6, ECO:0000269|PubMed:16971064, ECO:0000269|PubMed:18951094, ECO:0000269|PubMed:19703396, ECO:0000269|PubMed:22118463}.

Pfam Domains GO Terms

CRISPR Data

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
Hypoxia 5%O2 R04 exp191	-2.06
Cannabidiol 20μM R08 exp466	1.72
Geldanamycin 0.015 to 0.05μM on day4 R07 exp317	1.74
Heregulin-B 44ng/ml R08 exp486	1.81
PU-H71 1μM R08 exp515	1.83

Global Fraction of Cell Lines Where Essential: 1/739

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	0/1
909776.0	0/1
bile duct	0/28
blood	0/28
bone	0/26
breast	0/33
central nervous system	0/56
cervix	0/4
colorectal	0/17
esophagus	0/13
fibroblast	0/1
gastric	0/16
kidney	0/21
liver	1/20
lung	0/75
lymphocyte	0/16
ovary	0/26
pancreas	0/24
peripheral nervous system	0/16
plasma cell	0/15
prostate	0/1
skin	0/24
soft tissue	0/9
thyroid	0/2
upper aerodigestive	0/22
urinary tract	0/29
uterus	0/5

Essentiality in NALM6

Essentiality Rank: 15330
Expression level (log2 read counts): -4.6

Expression Distribution

miRNA catabolic process
pre-miRNA processing
miRNA metabolic process
ncRNA catabolic process
production of miRNAs involved in gene silencing by miRNA
dsRNA processing
production of small RNA involved in gene silencing by RNA
gene silencing by miRNA
posttranscriptional gene silencing by RNA
posttranscriptional gene silencing
gene silencing by RNA
RNA 3-end processing
gene silencing
regulation of gene expression, epigenetic
RNA catabolic process
nucleobase-containing compound catabolic process
ncRNA processing
heterocycle catabolic process
cellular nitrogen compound catabolic process
aromatic compound catabolic process
ncRNA metabolic process
organic cyclic compound catabolic process
posttranscriptional regulation of gene expression
zinc ion binding
nucleolus
RNA processing
cellular macromolecule catabolic process
macromolecule catabolic process
RNA binding
RNA metabolic process
negative regulation of gene expression
organic substance catabolic process
cellular catabolic process
gene expression