ARIH2

Gene Information

Official Symbol: ARIH2
Official Name: ariadne RBR E3 ubiquitin protein ligase 2
Aliases and Previous Symbols: N/A
Entrez ID: 10425
UniProt: O95376
Interactions: BioGRID
PubMed articles: Open PubMed
OMIM: Open OMIM

Function Summary

Entrez Summary: N/A
UniProt Summary: E3 ubiquitin-protein ligase, which catalyzes ubiquitination of target proteins together with ubiquitin- conjugating enzyme E2 UBE2L3 (PubMed:16118314, PubMed:17646546, PubMed:19340006, PubMed:24076655). Acts as an atypical E3 ubiquitin-protein ligase by working together with cullin-5-RING ubiquitin ligase complex (ECS complex, also named CRL5 complex) and initiating ubiquitination of ECS substrates: associates with ECS complex and specifically mediates addition of the first ubiquitin on ECS targets (By similarity). The initial ubiquitin is then elongated (By similarity). E3 ubiquitin-protein ligase activity is activated upon binding to neddylated form of the ECS complex (PubMed:24076655). Mediates 'Lys-6', 'Lys-48'-and 'Lys- 63'-linked polyubiquitination (PubMed:16118314, PubMed:17646546, PubMed:19340006). May play a role in myelopoiesis (PubMed:19340006). {ECO:0000250|UniProtKB:Q9Y4X5, ECO:0000269|PubMed:16118314, ECO:0000269|PubMed:17646546, ECO:0000269|PubMed:19340006, ECO:0000269|PubMed:24076655}.

Pfam Domains GO Terms

CRISPR Data

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
TNF-alpha 44ng/ml R08 exp533	-1.91
Rapamycin 2μM R07 exp306	-1.84
AICAR 240μM R03 exp116	-1.72
BI-D1870 2μM R04 exp162	1.75
Isonicotinamide 500μM R03 exp129	1.76
Thapsigargin 0.005μM R07 exp407	1.8
39°C R06 exp303	1.98

Global Fraction of Cell Lines Where Essential: 1/739

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	0/1
909776.0	0/1
bile duct	0/28
blood	0/28
bone	0/26
breast	0/33
central nervous system	0/56
cervix	0/4
colorectal	0/17
esophagus	0/13
fibroblast	0/1
gastric	0/16
kidney	0/21
liver	0/20
lung	0/75
lymphocyte	0/16
ovary	0/26
pancreas	0/24
peripheral nervous system	0/16
plasma cell	0/15
prostate	0/1
skin	0/24
soft tissue	0/9
thyroid	0/2
upper aerodigestive	0/22
urinary tract	0/29
uterus	0/5

Essentiality in NALM6

Essentiality Rank: 8758
Expression level (log2 read counts): 7.14

Expression Distribution

Cul5-RING ubiquitin ligase complex
hematopoietic stem cell proliferation
positive regulation of protein targeting to mitochondrion
ubiquitin conjugating enzyme binding
protein K63-linked ubiquitination
regulation of protein targeting to mitochondrion
stem cell proliferation
protein K48-linked ubiquitination
positive regulation of establishment of protein localization to mitochondrion
regulation of establishment of protein localization to mitochondrion
regulation of protein targeting
positive regulation of proteasomal ubiquitin-dependent protein catabolic process
developmental cell growth
cell growth
positive regulation of ubiquitin-dependent protein catabolic process
positive regulation of proteasomal protein catabolic process
ubiquitin ligase complex
positive regulation of mitochondrion organization
positive regulation of proteolysis involved in cellular protein catabolic process
regulation of proteasomal ubiquitin-dependent protein catabolic process
positive regulation of cellular protein catabolic process
regulation of ubiquitin-dependent protein catabolic process
positive regulation of intracellular protein transport
regulation of mitochondrion organization
regulation of proteasomal protein catabolic process
positive regulation of intracellular transport
positive regulation of protein catabolic process
regulation of proteolysis involved in cellular protein catabolic process
ubiquitin protein ligase activity
regulation of intracellular protein transport
ubiquitin-protein transferase activity
regulation of cellular protein catabolic process
protein polyubiquitination
positive regulation of cellular protein localization
regulation of intracellular transport
positive regulation of proteolysis
positive regulation of cellular catabolic process
regulation of protein catabolic process
developmental growth
growth