HLTF

Entrez Summary: N/A
UniProt Summary: Has both helicase and E3 ubiquitin ligase activities. Possesses intrinsic ATP-dependent nucleosome-remodeling activity; This activity may be required for transcriptional activation or repression of specific target promoters (By similarity). These may include the SERPINE1 and HIV-1 promoters and the SV40 enhancer, to which this protein can bind directly. Plays a role in error-free postreplication repair (PRR) of damaged DNA and maintains genomic stability through acting as a ubiquitin ligase for 'Lys-63'-linked polyubiquitination of chromatin-bound PCNA. {ECO:0000250, ECO:0000269|PubMed:10391891, ECO:0000269|PubMed:18316726, ECO:0000269|PubMed:18719106, ECO:0000269|PubMed:7876228, ECO:0000269|PubMed:8672239, ECO:0000269|PubMed:9126292}.

Pfam Domains GO Terms

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
Beta-Estradiol 0.55μM R07 exp425	-1.83
Campthothecin 0.001μM R06 exp270	-1.76
Mitomycin-C 0.06μM R08 exp503	-1.76
DABN racemic mixture R03 exp93	-1.72
4-Nitroquinoline-1-oxide 0.5μM R08 exp446	-1.71
INK128 0.2μM R02 exp70	-1.7
Phorbol-12-myristate-13-acetate 0.57μM R08 exp514	1.83
Oligomycin-A 20μM R00 exp26	2.44

Global Fraction of Cell Lines Where Essential: 0/726

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	0/1
909776.0	0/1
bile duct	0/28
blood	0/28
bone	0/25
breast	0/33
central nervous system	0/56
cervix	0/4
colorectal	0/17
esophagus	0/13
fibroblast	0/1
gastric	0/15
kidney	0/21
liver	0/20
lung	0/75
lymphocyte	0/14
ovary	0/26
pancreas	0/24
peripheral nervous system	0/16
plasma cell	0/15
prostate	0/1
skin	0/24
soft tissue	0/7
thyroid	0/2
upper aerodigestive	0/22
urinary tract	0/29
uterus	0/5

Expression Distribution

helicase activity
ubiquitin protein ligase activity
ubiquitin protein ligase binding
DNA-binding transcription activator activity, RNA polymerase II-specific
RNA polymerase II proximal promoter sequence-specific DNA binding
protein ubiquitination
chromatin organization
protein modification by small protein conjugation
zinc ion binding
nucleolus
protein modification by small protein conjugation or removal
chromosome organization
positive regulation of transcription by RNA polymerase II
RNA binding
DNA binding
ATP binding
positive regulation of transcription, DNA-templated
positive regulation of nucleic acid-templated transcription
positive regulation of RNA biosynthetic process
positive regulation of RNA metabolic process
positive regulation of nucleobase-containing compound metabolic process
positive regulation of macromolecule biosynthetic process
positive regulation of cellular biosynthetic process
positive regulation of gene expression
membrane
positive regulation of biosynthetic process