PARP1
Gene Information
- Official Symbol: PARP1
- Official Name: poly(ADP-ribose) polymerase 1
- Aliases and Previous Symbols: N/A
- Entrez ID: 142
- UniProt: P09874
- Interactions: BioGRID
- PubMed articles: Open PubMed
- OMIM: Open OMIM
Function Summary
- Entrez Summary: N/A
- UniProt Summary: Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks (PubMed:17177976, PubMed:18172500, PubMed:19344625, PubMed:19661379, PubMed:23230272). Mediates the poly(ADP-ribosyl)ation of APLF and CHFR (PubMed:17396150). Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production (PubMed:17177976). Required for PARP9 and DTX3L recruitment to DNA damage sites (PubMed:23230272). PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites (PubMed:23230272). Mediates serine ADP-ribosylation of target proteins following interaction with HPF1; HPF1 conferring serine specificity (PubMed:28190768). Mediates the poly(ADP-ribosyl)ation of histones in a HPF1-dependent manner (PubMed:27067600). Involved in the synthesis of ATP in the nucleus, together with NMNAT1, PARG and NUDT5 (PubMed:27257257). Nuclear ATP generation is required for extensive chromatin remodeling events that are energy- consuming (PubMed:27257257). {ECO:0000269|PubMed:17177976, ECO:0000269|PubMed:17396150, ECO:0000269|PubMed:18172500, ECO:0000269|PubMed:19344625, ECO:0000269|PubMed:19661379, ECO:0000269|PubMed:23230272, ECO:0000269|PubMed:27067600, ECO:0000269|PubMed:27257257, ECO:0000269|PubMed:28190768}.
Pfam Domains GO Terms
Pfam Domains
| PARP |
| PARP reg |
| PADR1 |
| BRCT |
| WGR |
| zf-PARP |
GO Terms
| peptidyl-glutamic acid poly-ADP-ribosylation |
| positive regulation of myofibroblast differentiation |
| positive regulation of single strand break repair |
| DNA ADP-ribosylation |
| NAD DNA ADP-ribosyltransferase activity |
| regulation of single strand break repair |
| negative regulation of ATP biosynthetic process |
| response to aldosterone |
| ATP generation from poly-ADP-D-ribose |
| regulation of myofibroblast differentiation |
| peptidyl-serine ADP-ribosylation |
| mitochondrial DNA repair |
| negative regulation of purine nucleotide biosynthetic process |
| negative regulation of nucleotide biosynthetic process |
| regulation of SMAD protein complex assembly |
| regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway |
| protein poly-ADP-ribosylation |
| mitochondrial DNA metabolic process |
| positive regulation of double-strand break repair via homologous recombination |
| positive regulation of mitochondrial depolarization |
| protein auto-ADP-ribosylation |
| positive regulation of membrane depolarization |
| positive regulation of intracellular estrogen receptor signaling pathway |
| positive regulation of SMAD protein signal transduction |
| positive regulation of intracellular steroid hormone receptor signaling pathway |
| protein autoprocessing |
| protein ADP-ribosylase activity |
| regulation of ATP biosynthetic process |
| negative regulation of purine nucleotide metabolic process |
| site of DNA damage |
| mitochondrial genome maintenance |
| negative regulation of nucleotide metabolic process |
| signal transduction involved in regulation of gene expression |
| nucleotide-excision repair, preincision complex stabilization |
| regulation of mitochondrial depolarization |
| regulation of SMAD protein signal transduction |
| nucleotide-excision repair, DNA duplex unwinding |
| nucleotide-excision repair, DNA incision, 3-to lesion |
| R-SMAD binding |
| nucleotide-excision repair, DNA damage recognition |
| cellular response to zinc ion |
| regulation of DNA methylation |
| macrophage differentiation |
| positive regulation of transcription regulatory region DNA binding |
| negative regulation of ATP metabolic process |
| global genome nucleotide-excision repair |
| regulation of oxidative stress-induced neuron death |
| negative regulation of telomere maintenance via telomere lengthening |
| NAD+ ADP-ribosyltransferase activity |
| regulation of oxidative stress-induced intrinsic apoptotic signaling pathway |
| nucleotide-excision repair, preincision complex assembly |
| peptidyl-glutamic acid modification |
| protein ADP-ribosylation |
| positive regulation of cardiac muscle hypertrophy |
| positive regulation of double-strand break repair |
| positive regulation of muscle hypertrophy |
| response to mineralocorticoid |
| negative regulation of telomere maintenance |
| nucleotide-excision repair, DNA incision, 5-to lesion |
| regulation of purine nucleotide biosynthetic process |
| cellular response to amyloid-beta |
| DNA damage response, detection of DNA damage |
| positive regulation of DNA recombination |
| regulation of nucleotide biosynthetic process |
| nucleotide-excision repair, DNA incision |
| NAD binding |
| regulation of intracellular estrogen receptor signaling pathway |
| estrogen receptor binding |
| regulation of membrane depolarization |
| protein-DNA complex |
| regulation of double-strand break repair via homologous recombination |
| response to amyloid-beta |
| regulation of transcription regulatory region DNA binding |
| response to zinc ion |
| response to gamma radiation |
| regulation of cardiac muscle hypertrophy |
| positive regulation of DNA binding |
| site of double-strand break |
| regulation of muscle hypertrophy |
| regulation of telomere maintenance via telomere lengthening |
| regulation of oxidative stress-induced cell death |
| positive regulation of DNA repair |
| regulation of mitochondrial membrane potential |
| positive regulation of protein localization to nucleus |
| regulation of cellular response to oxidative stress |
| regulation of intracellular steroid hormone receptor signaling pathway |
| regulation of double-strand break repair |
| regulation of telomere maintenance |
| cellular response to UV |
| regulation of response to oxidative stress |
| regulation of muscle adaptation |
| DNA modification |
| negative regulation of small molecule metabolic process |
| positive regulation of neuron death |
| double-strand break repair via homologous recombination |
| telomere maintenance |
| recombinational repair |
| positive regulation of response to DNA damage stimulus |
| telomere organization |
| transforming growth factor beta receptor signaling pathway |
| positive regulation of transmembrane receptor protein serine/threonine kinase signaling pathway |
| regulation of DNA recombination |
| protein N-terminus binding |
| nuclear chromosome, telomeric region |
| DNA duplex unwinding |
| nucleotide-excision repair |
| myeloid leukocyte differentiation |
| histone deacetylase binding |
| cellular response to light stimulus |
| regulation of protein localization to nucleus |
| regulation of purine nucleotide metabolic process |
| regulation of nucleotide metabolic process |
| DNA geometric change |
| regulation of ATP metabolic process |
| negative regulation of DNA metabolic process |
| regulation of DNA repair |
| regulation of DNA binding |
| negative regulation of chromosome organization |
| response to UV |
| response to ionizing radiation |
| cellular response to transforming growth factor beta stimulus |
| protein processing |
| regulation of intrinsic apoptotic signaling pathway |
| response to corticosteroid |
| response to transforming growth factor beta |
| cellular response to insulin stimulus |
| cellular response to radiation |
| positive regulation of binding |
| nuclear envelope |
| double-strand break repair |
| cellular response to metal ion |
| response to ketone |
| positive regulation of DNA metabolic process |
| transmembrane receptor protein serine/threonine kinase signaling pathway |
| peptidyl-serine modification |
| protein-DNA complex assembly |
| ATP metabolic process |
| transcription factor complex |
| regulation of neuron apoptotic process |
| regulation of response to DNA damage stimulus |
| myeloid cell differentiation |
| cellular response to inorganic substance |
| DNA recombination |
| protein maturation |
| response to insulin |
| response to alcohol |
| regulation of transmembrane receptor protein serine/threonine kinase signaling pathway |
| regulation of muscle system process |
| cellular response to oxidative stress |
| protein-DNA complex subunit organization |
| positive regulation of protein complex assembly |
| cellular response to peptide hormone stimulus |
| DNA conformation change |
| nucleic acid phosphodiester bond hydrolysis |
| response to light stimulus |
| regulation of neuron death |
| RNA polymerase II regulatory region sequence-specific DNA binding |
| cellular response to abiotic stimulus |
| cellular response to environmental stimulus |
| positive regulation of cellular protein localization |
| cellular response to peptide |
| response to steroid hormone |
| leukocyte differentiation |
| transcription factor binding |
| anatomical structure homeostasis |
| enzyme binding |
| regulation of chromosome organization |
| regulation of DNA metabolic process |
| response to metal ion |
| negative regulation of organelle organization |
| regulation of binding |
| response to oxidative stress |
| response to peptide hormone |
| regulation of apoptotic signaling pathway |
| regulation of small molecule metabolic process |
| regulation of membrane potential |
| response to radiation |
| mitochondrion organization |
| DNA-binding transcription activator activity, RNA polymerase II-specific |
| regulation of protein complex assembly |
| protein kinase binding |
| response to peptide |
| transcription by RNA polymerase II |
| cellular response to growth factor stimulus |
| DNA repair |
| positive regulation of cellular component biogenesis |
| response to growth factor |
| response to inorganic substance |
| regulation of cellular protein localization |
| hemopoiesis |
| negative regulation of phosphate metabolic process |
| negative regulation of phosphorus metabolic process |
| regulation of system process |
| protein-containing complex |
| cellular response to organonitrogen compound |
| cellular response to hormone stimulus |
| hematopoietic or lymphoid organ development |
| transcription, DNA-templated |
| nucleic acid-templated transcription |
| immune system development |
| RNA biosynthetic process |
| cellular response to nitrogen compound |
| detection of stimulus |
| positive regulation of cell death |
| negative regulation of cellular component organization |
| enzyme linked receptor protein signaling pathway |
| regulation of cellular response to stress |
| DNA metabolic process |
| cellular response to DNA damage stimulus |
| ion homeostasis |
| zinc ion binding |
| cellular protein-containing complex assembly |
| response to lipid |
| nucleolus |
| negative regulation of transcription by RNA polymerase II |
| peptidyl-amino acid modification |
| response to hormone |
| regulation of cellular localization |
| response to organic cyclic compound |
| apoptotic process |
| regulation of cellular component biogenesis |
| positive regulation of cell differentiation |
| response to organonitrogen compound |
| carbohydrate derivative metabolic process |
| regulation of protein localization |
| programmed cell death |
| cellular response to oxygen-containing compound |
| chromosome organization |
| identical protein binding |
| response to nitrogen compound |
| cell death |
| nucleobase-containing compound biosynthetic process |
| chemical homeostasis |
| response to abiotic stimulus |
| heterocycle biosynthetic process |
| aromatic compound biosynthetic process |
| negative regulation of transcription, DNA-templated |
| positive regulation of cellular component organization |
| positive regulation of transcription by RNA polymerase II |
| cellular response to endogenous stimulus |
| negative regulation of nucleic acid-templated transcription |
| negative regulation of RNA biosynthetic process |
| mitochondrion |
| proteolysis |
| regulation of organelle organization |
| organic cyclic compound biosynthetic process |
| negative regulation of RNA metabolic process |
| positive regulation of developmental process |
| negative regulation of cellular macromolecule biosynthetic process |
| RNA binding |
| negative regulation of nucleobase-containing compound metabolic process |
| DNA binding |
| negative regulation of macromolecule biosynthetic process |
| response to endogenous stimulus |
| regulation of response to stress |
| negative regulation of cellular biosynthetic process |
| regulation of apoptotic process |
| positive regulation of transcription, DNA-templated |
| negative regulation of biosynthetic process |
| response to oxygen-containing compound |
| protein-containing complex assembly |
| regulation of programmed cell death |
| cellular nitrogen compound biosynthetic process |
| positive regulation of nucleic acid-templated transcription |
| positive regulation of RNA biosynthetic process |
| homeostatic process |
| positive regulation of signal transduction |
| RNA metabolic process |
| regulation of cell death |
| cellular response to stress |
| cellular macromolecule biosynthetic process |
| negative regulation of gene expression |
| positive regulation of RNA metabolic process |
| positive regulation of multicellular organismal process |
| macromolecule biosynthetic process |
| positive regulation of molecular function |
| regulation of phosphate metabolic process |
| regulation of phosphorus metabolic process |
| regulation of cell differentiation |
| positive regulation of cell communication |
| positive regulation of signaling |
| regulation of intracellular signal transduction |
| protein-containing complex subunit organization |
| positive regulation of nucleobase-containing compound metabolic process |
| positive regulation of macromolecule biosynthetic process |
| positive regulation of cellular biosynthetic process |
| positive regulation of gene expression |
| membrane |
| gene expression |
| positive regulation of biosynthetic process |
CRISPR Data
Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)
Compound Hit
| Screen | Score |
|---|---|
| Temozolomide 200μM R04 exp189 | -2.85 |
| Campthothecin 0.001μM R06 exp270 | -2.44 |
| Deferoxamine 11μM R07 exp350 | -2.14 |
| Zebularine 20μM R07 exp423 | -1.94 |
| Bortezomib 0.05μM R00 exp7 | -1.86 |
| BI-6727 0.04μM R03 exp98 | 1.71 |
| Rucaparib 6.5μM R08 exp520 | 7.51 |
| Olaparib 4μM R08 exp512 | 7.76 |
Most Correlated Genes in Chemogenomics
| Gene | Correlation |
|---|---|
| ADPRHL2 | 0.434 |
Tissues where Essential in the Avana Dataset (DepMap 20Q1)
Global Fraction of Cell Lines Where Essential: 0/726
| Tissue | Fraction Of Cell Lines Where Essential |
|---|---|
| 1290807.0 | 0/1 |
| 909776.0 | 0/1 |
| bile duct | 0/28 |
| blood | 0/28 |
| bone | 0/25 |
| breast | 0/33 |
| central nervous system | 0/56 |
| cervix | 0/4 |
| colorectal | 0/17 |
| esophagus | 0/13 |
| fibroblast | 0/1 |
| gastric | 0/15 |
| kidney | 0/21 |
| liver | 0/20 |
| lung | 0/75 |
| lymphocyte | 0/14 |
| ovary | 0/26 |
| pancreas | 0/24 |
| peripheral nervous system | 0/16 |
| plasma cell | 0/15 |
| prostate | 0/1 |
| skin | 0/24 |
| soft tissue | 0/7 |
| thyroid | 0/2 |
| upper aerodigestive | 0/22 |
| urinary tract | 0/29 |
| uterus | 0/5 |
Essentiality in NALM6
- Essentiality Rank: 3617
- Expression level (log2 read counts): 9.61
Expression Distribution
PARP1 Expression in NALM6 Cells: 9.61