PIF1

Entrez Summary: N/A
UniProt Summary: DNA-dependent ATPase and 5'-3' DNA helicase required for the maintenance of both mitochondrial and nuclear genome stability. Efficiently unwinds G-quadruplex (G4) DNA structures and forked RNA-DNA hybrids. Resolves G4 structures, preventing replication pausing and double-strand breaks (DSBs) at G4 motifs. Involved in the maintenance of telomeric DNA. Inhibits telomere elongation, de novo telomere formation and telomere addition to DSBs via catalytic inhibition of telomerase. Reduces the processivity of telomerase by displacing active telomerase from DNA ends. Releases telomerase by unwinding the short telomerase RNA/telomeric DNA hybrid that is the intermediate in the telomerase reaction. Possesses an intrinsic strand annealing activity. {ECO:0000255|HAMAP-Rule:MF_03176, ECO:0000269|PubMed:16522649, ECO:0000269|PubMed:17172855, ECO:0000269|PubMed:17827721, ECO:0000269|PubMed:18835853, ECO:0000269|PubMed:19700773, ECO:0000269|PubMed:20524933, ECO:0000269|PubMed:23657261}.

Pfam Domains GO Terms

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Global Fraction of Cell Lines Where Essential: 0/726

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	0/1
909776.0	0/1
bile duct	0/28
blood	0/28
bone	0/25
breast	0/33
central nervous system	0/56
cervix	0/4
colorectal	0/17
esophagus	0/13
fibroblast	0/1
gastric	0/15
kidney	0/21
liver	0/20
lung	0/75
lymphocyte	0/14
ovary	0/26
pancreas	0/24
peripheral nervous system	0/16
plasma cell	0/15
prostate	0/1
skin	0/24
soft tissue	0/7
thyroid	0/2
upper aerodigestive	0/22
urinary tract	0/29
uterus	0/5

Expression Distribution

Screen	Score
Atovaquone 15μM R08 exp451	-2
Cidofovir 10μM R07 exp345	1.82

5-3 DNA/RNA helicase activity
telomerase inhibitor activity
5-3 DNA helicase activity
G-quadruplex DNA binding
negative regulation of telomerase activity
single-stranded DNA helicase activity
mitochondrial genome maintenance
negative regulation of telomere maintenance via telomerase
replication fork
negative regulation of telomere maintenance via telomere lengthening
telomeric DNA binding
negative regulation of telomere maintenance
negative regulation of DNA biosynthetic process
regulation of telomerase activity
regulation of telomere maintenance via telomerase
regulation of telomere maintenance via telomere lengthening
regulation of telomere maintenance
telomere maintenance
telomere organization
nuclear chromosome, telomeric region
DNA duplex unwinding
regulation of DNA biosynthetic process
DNA geometric change
negative regulation of DNA metabolic process
negative regulation of chromosome organization
DNA replication
magnesium ion binding
DNA recombination
negative regulation of transferase activity
DNA conformation change
anatomical structure homeostasis
regulation of chromosome organization
regulation of DNA metabolic process
negative regulation of organelle organization
mitochondrion organization
DNA repair
negative regulation of cellular component organization
DNA metabolic process
cellular response to DNA damage stimulus
negative regulation of catalytic activity