PUM2

Gene Information

Official Symbol: PUM2
Official Name: pumilio RNA binding family member 2
Aliases and Previous Symbols: N/A
Entrez ID: 23369
UniProt: Q8TB72
Interactions: BioGRID
PubMed articles: Open PubMed
OMIM: Open OMIM

Function Summary

Entrez Summary: N/A
UniProt Summary: Sequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the 3'-UTR of mRNA targets. Binds to an RNA consensus sequence, the Pumilio Response Element (PRE), 5'-UGUANAUA-3', that is related to the Nanos Response Element (NRE) (, PubMed:21397187). Mediates post- transcriptional repression of transcripts via different mechanisms: acts via direct recruitment of the CCR4-POP2-NOT deadenylase leading to translational inhibition and mRNA degradation (PubMed:22955276). Also mediates deadenylation- independent repression by promoting accessibility of miRNAs (PubMed:18776931, PubMed:22345517). Acts as a post-transcriptional repressor of E2F3 mRNAs by binding to its 3'-UTR and facilitating miRNA regulation (PubMed:22345517). Plays a role in cytoplasmic sensing of viral infection (PubMed:25340845). Represses a program of genes necessary to maintain genomic stability such as key mitotic, DNA repair and DNA replication factors. Its ability to repress those target mRNAs is regulated by the lncRNA NORAD (non- coding RNA activated by DNA damage) which, due to its high abundance and multitude of PUMILIO binding sites, is able to sequester a significant fraction of PUM1 and PUM2 in the cytoplasm (PubMed:26724866). May regulate DCUN1D3 mRNA levels (PubMed:25349211). May support proliferation and self-renewal of stem cells. Binds specifically to miRNA MIR199A precursor, with PUM1, regulates miRNA MIR199A expression at a postranscriptional level (PubMed:28431233). {ECO:0000269|PubMed:18776931, ECO:0000269|PubMed:21397187, ECO:0000269|PubMed:22345517, ECO:0000269|PubMed:22955276, ECO:0000269|PubMed:25340845, ECO:0000269|PubMed:25349211, ECO:0000269|PubMed:26724866, ECO:0000269|PubMed:28431233}.

Pfam Domains GO Terms

CRISPR Data

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
Clomiphene 4.4μM R02 exp68	-1.79
Vinblastine 0.2μM R03 exp108	-1.79
Celastrol 0.12μM R05 exp225	-1.7
THZ531 0.225μM R08 exp444	1.77
Mdivi-1 15μM R05 exp217	1.83
UMK57 1μM R01 exp59	2.14

Global Fraction of Cell Lines Where Essential: 0/739

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	0/1
909776.0	0/1
bile duct	0/28
blood	0/28
bone	0/26
breast	0/33
central nervous system	0/56
cervix	0/4
colorectal	0/17
esophagus	0/13
fibroblast	0/1
gastric	0/16
kidney	0/21
liver	0/20
lung	0/75
lymphocyte	0/16
ovary	0/26
pancreas	0/24
peripheral nervous system	0/16
plasma cell	0/15
prostate	0/1
skin	0/24
soft tissue	0/9
thyroid	0/2
upper aerodigestive	0/22
urinary tract	0/29
uterus	0/5

Essentiality in NALM6

Essentiality Rank: 15962
Expression level (log2 read counts): 7.57

Expression Distribution

positive regulation of RIG-I signaling pathway
stress granule assembly
regulation of RIG-I signaling pathway
regulation of viral-induced cytoplasmic pattern recognition receptor signaling pathway
positive regulation of gene silencing by miRNA
positive regulation of posttranscriptional gene silencing
production of miRNAs involved in gene silencing by miRNA
miRNA binding
dsRNA processing
production of small RNA involved in gene silencing by RNA
gene silencing by miRNA
posttranscriptional gene silencing by RNA
posttranscriptional gene silencing
cytoplasmic stress granule
regulation of defense response to virus
mRNA 3-UTR binding
regulation of gene silencing by miRNA
gene silencing by RNA
regulation of gene silencing by RNA
regulation of posttranscriptional gene silencing
regulation of chromosome segregation
regulation of gene silencing
gene silencing
regulation of mRNA stability
regulation of RNA stability
regulation of mRNA catabolic process
positive regulation of immune effector process
ribonucleoprotein complex assembly
regulation of gene expression, epigenetic
nuclear membrane
ribonucleoprotein complex subunit organization
regulation of mRNA metabolic process
positive regulation of innate immune response
regulation of translation
positive regulation of response to biotic stimulus
regulation of cellular amide metabolic process
positive regulation of cytokine production
regulation of innate immune response
ribonucleoprotein complex biogenesis
regulation of immune effector process