TRMT12

Entrez Summary: N/A
UniProt Summary: S-adenosyl-L-methionine-dependent transferase that acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. Catalyzes the transfer of the alpha-amino-alpha- carboxypropyl (acp) group from S-adenosyl-L-methionine to the C-7 position of 4-demethylwyosine (imG-14) to produce wybutosine-86. {ECO:0000269|PubMed:22761755}.

Pfam Domains GO Terms

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
Ethambutol 25μM R08 exp481	-2.45
4-Nitroquinoline-1-oxide 0.5μM R08 exp446	-2.01
Tozasertib 0.1μM R07 exp414	-1.81
Trimetrexate 0.03μM R08 exp535	-1.74

Global Fraction of Cell Lines Where Essential: 0/739

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	0/1
909776.0	0/1
bile duct	0/28
blood	0/28
bone	0/26
breast	0/33
central nervous system	0/56
cervix	0/4
colorectal	0/17
esophagus	0/13
fibroblast	0/1
gastric	0/16
kidney	0/21
liver	0/20
lung	0/75
lymphocyte	0/16
ovary	0/26
pancreas	0/24
peripheral nervous system	0/16
plasma cell	0/15
prostate	0/1
skin	0/24
soft tissue	0/9
thyroid	0/2
upper aerodigestive	0/22
urinary tract	0/29
uterus	0/5

Expression Distribution

tRNA 4-demethylwyosine alpha-amino-alpha-carboxypropyltransferase activity
wybutosine metabolic process
wybutosine biosynthetic process
tRNA methyltransferase activity
tRNA methylation
glycosyl compound biosynthetic process
RNA methylation
tRNA modification
tRNA processing
glycosyl compound metabolic process
RNA modification
tRNA metabolic process
macromolecule methylation
methylation
ncRNA processing
ncRNA metabolic process
carbohydrate derivative biosynthetic process
RNA processing
carbohydrate derivative metabolic process
organonitrogen compound biosynthetic process
RNA metabolic process
gene expression