WRN

Gene Information

Official Symbol: WRN
Official Name: WRN RecQ like helicase
Aliases and Previous Symbols: N/A
Entrez ID: 7486
UniProt: Q14191
Interactions: BioGRID
PubMed articles: Open PubMed
OMIM: Open OMIM

Function Summary

Entrez Summary: This gene encodes a member of the RecQ subfamily of DNA helicase proteins. The encoded nuclear protein is important in the maintenance of genome stability and plays a role in DNA repair, replication, transcription and telomere maintenance. This protein contains a N-terminal 3' to 5' exonuclease domain, an ATP-dependent helicase domain and RQC (RecQ helicase conserved region) domain in its central region, and a C-terminal HRDC (helicase RNase D C-terminal) domain and nuclear localization signal. Defects in this gene are the cause of Werner syndrome, an autosomal recessive disorder characterized by accelerated aging and an elevated risk for certain cancers. [provided by RefSeq, Aug 2017].
UniProt Summary: Multifunctional enzyme that has both magnesium and ATP- dependent DNA-helicase activity and 3'→5' exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double- stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A (By similarity). Plays a role in double-strand break repair after gamma-irradiation. {ECO:0000250, ECO:0000269|PubMed:11863428, ECO:0000269|PubMed:17563354, ECO:0000269|PubMed:18596042, ECO:0000269|PubMed:19283071, ECO:0000269|PubMed:19652551, ECO:0000269|PubMed:21639834}.

Pfam Domains GO Terms

CRISPR Data

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Global Fraction of Cell Lines Where Essential: 9/739

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	0/1
909776.0	0/1
bile duct	0/28
blood	0/28
bone	0/26
breast	0/33
central nervous system	0/56
cervix	0/4
colorectal	0/17
esophagus	0/13
fibroblast	0/1
gastric	0/16
kidney	0/21
liver	0/20
lung	0/75
lymphocyte	0/16
ovary	0/26
pancreas	0/24
peripheral nervous system	0/16
plasma cell	0/15
prostate	0/1
skin	0/24
soft tissue	0/9
thyroid	0/2
upper aerodigestive	0/22
urinary tract	0/29
uterus	0/5

Essentiality in NALM6

Essentiality Rank: 9848
Expression level (log2 read counts): 5.95

Expression Distribution

DEAD
RQC
DNA pol A exo1
HRDC
Helicase C

3-flap-structured DNA binding
regulation of strand invasion
positive regulation of strand invasion
forked DNA-dependent helicase activity
telomeric G-quadruplex DNA binding
8-hydroxy-2-deoxyguanosine DNA binding
telomeric D-loop binding
four-way junction helicase activity
replicative cell aging
Y-form DNA binding
G-quadruplex DNA unwinding
protein localization to nucleolus
MutLalpha complex binding
regulation of growth rate
bubble DNA binding
telomeric D-loop disassembly
G-quadruplex DNA binding
t-circle formation
telomere maintenance via telomere trimming
formation of extrachromosomal circular DNA
telomeric loop disassembly
DNA unwinding involved in DNA replication
exonuclease activity
response to UV-C
four-way junction DNA binding
3-5 DNA helicase activity
3-5 exonuclease activity
replication fork
cellular response to gamma radiation
replication fork processing
helicase activity
multicellular organism aging
base-excision repair
positive regulation of DNA recombination
DNA-dependent DNA replication maintenance of fidelity
chromosome, telomeric region
DNA synthesis involved in DNA repair
response to gamma radiation
DNA helicase activity
manganese ion binding