WDR61

Gene Information

Official Symbol: WDR61
Official Name: WD repeat domain 61
Aliases and Previous Symbols: N/A
Entrez ID: 80349
UniProt: Q9GZS3
Interactions: BioGRID
PubMed articles: Open PubMed
OMIM: N/A

Function Summary

Entrez Summary: N/A
UniProt Summary: Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non- phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1; it promotes leukemogenesis through association with KMT2A/MLL1-rearranged oncoproteins, such as KMT2A/MLL1-MLLT3/AF9 and KMT2A/MLL1-MLLT1/ENL. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regulating gene transcription. Required for mono- and trimethylation on histone H3 'Lys-4' (H3K4me3), dimethylation on histone H3 'Lys-79' (H3K4me3). Required for Hox gene transcription. Component of the SKI complex which is thought to be involved in exosome-mediated RNA decay and associates with transcriptionally active genes in a manner dependent on PAF1C. {ECO:0000269|PubMed:16024656, ECO:0000269|PubMed:16307923, ECO:0000269|PubMed:19952111, ECO:0000269|PubMed:20178742}.

Pfam Domains GO Terms

CRISPR Data

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
UMK57 1μM R01 exp59	-3.27
THZ1 0.06μM R08 exp531	-2.73
DABN racemic mixture R03 exp93	-2.24
SNS-032 25μM R07 exp401	-2.15
Sulforaphane 9μM R08 exp526 no dilution day6	-2.12
Hinokiflavone 12μM R08 exp487	-2.1
BI-6727 0.04μM R03 exp98	-2.08
Q15 2.7μM R02 exp79	-2.07
Mubritinib 0.2μM R01 exp48	-2.06
Pimelic-diphenylamide-106 0.5μM R00 exp27	-2.05
Campthothecin 0.001μM R06 exp270	-1.92
Nocodazole 0.2μM R02 exp64	-1.76
Glyphosate 1000μM R06 exp283	-1.74
PNU96415E 10μM R03 exp145	-1.73
Rotenone 20μM R00 exp34	-1.72
NFN1 0.4μM R03 exp99	-1.71
6-Thio-2-deoxyguanosine 2μM R06 exp259	-1.7
CoCl2 18μM R07 exp346	-1.7
Cytochalasin-B 5μM R07 exp349	1.83

Gene	Correlation
CDC73	0.476
CTR9	0.407
NARFL	0.407
INTS5	0.406
NUP160	0.401
INTS8	0.4

Global Fraction of Cell Lines Where Essential: 81/726

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	0/1
909776.0	0/1
bile duct	2/28
blood	1/28
bone	4/25
breast	12/33
central nervous system	6/56
cervix	1/4
colorectal	0/17
esophagus	0/13
fibroblast	0/1
gastric	1/15
kidney	5/21
liver	3/20
lung	5/75
lymphocyte	3/14
ovary	0/26
pancreas	0/24
peripheral nervous system	1/16
plasma cell	5/15
prostate	0/1
skin	1/24
soft tissue	0/7
thyroid	0/2
upper aerodigestive	4/22
urinary tract	6/29
uterus	1/5

Essentiality in NALM6

Essentiality Rank: 518
Expression level (log2 read counts): 6.21

Expression Distribution