NELFE

Gene Information

Official Symbol: NELFE
Official Name: negative elongation factor complex member E
Aliases and Previous Symbols: N/A
Entrez ID: 7936
UniProt: P18615
Interactions: BioGRID
PubMed articles: Open PubMed
OMIM: Open OMIM

Function Summary

Entrez Summary: N/A
UniProt Summary: Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II. Provides the strongest RNA binding activity of the NELF complex and may initially recruit the NELF complex to RNA. Binds to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1. {ECO:0000269|PubMed:10199401, ECO:0000269|PubMed:11940650, ECO:0000269|PubMed:12612062, ECO:0000269|PubMed:18303858, ECO:0000269|PubMed:27282391, ECO:0000305}.

Pfam Domains GO Terms

CRISPR Data

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
UMK57 1μM R01 exp59	-2.76
HMS-I1 1μM R01 exp46	-2.5
BML-284 0.09μM R08 exp460	-2.24
Deferoxamine 11μM R07 exp350	-2.19
LY2090314 0.003μM R08 exp500 no dilution day6	-2.05
Hinokiflavone 12μM R08 exp487	-2.03
Vinblastine 0.002μM R01 exp60	-2
JQ1 0.8μM R08 exp435	-1.93
Genistein 15μM R07 exp360	-1.88
Docetaxel 0.002μM R01 exp45	-1.8
Hippuristanol 0.12μM R08 exp489 no dilution day6	-1.73
LLY-283 2.6μM R06 exp290	-1.71

Global Fraction of Cell Lines Where Essential: 9/726

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	0/1
909776.0	0/1
bile duct	1/28
blood	1/28
bone	0/25
breast	0/33
central nervous system	0/56
cervix	0/4
colorectal	0/17
esophagus	0/13
fibroblast	0/1
gastric	0/15
kidney	0/21
liver	0/20
lung	1/75
lymphocyte	1/14
ovary	0/26
pancreas	0/24
peripheral nervous system	1/16
plasma cell	0/15
prostate	0/1
skin	2/24
soft tissue	0/7
thyroid	0/2
upper aerodigestive	0/22
urinary tract	0/29
uterus	0/5

Essentiality in NALM6

Essentiality Rank: 2300
Expression level (log2 read counts): 5.77

Expression Distribution

Gene	Correlation
NELFA	0.487
PDS5B	0.413

NELF complex
negative regulation of mRNA polyadenylation
negative regulation of mRNA 3-end processing
negative regulation of transcription elongation from RNA polymerase II promoter
negative regulation of DNA-templated transcription, elongation
positive regulation of histone H3-K4 methylation
regulation of mRNA polyadenylation
regulation of histone H3-K4 methylation
negative regulation of mRNA processing
regulation of mRNA 3-end processing
regulation of transcription elongation from RNA polymerase II promoter
positive regulation of histone methylation
positive regulation of viral transcription
regulation of DNA-templated transcription, elongation
regulation of viral transcription
regulation of histone methylation
transcription elongation from RNA polymerase II promoter
negative regulation of mRNA metabolic process
positive regulation of histone modification
DNA-templated transcription, elongation
positive regulation of chromatin organization
positive regulation of viral process
regulation of mRNA processing
regulation of histone modification
positive regulation of chromosome organization
regulation of chromatin organization
regulation of viral process
positive regulation of ERK1 and ERK2 cascade
regulation of symbiosis, encompassing mutualism through parasitism
nuclear body
regulation of ERK1 and ERK2 cascade
regulation of mRNA metabolic process
regulation of chromosome organization
chromatin binding
transcription by RNA polymerase II
positive regulation of multi-organism process
positive regulation of MAPK cascade
positive regulation of organelle organization
transcription, DNA-templated
nucleic acid-templated transcription