UBE2W

Entrez Summary: N/A
UniProt Summary: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins (PubMed:20061386). Specifically monoubiquitinates the N-terminus of various substrates, including ATXN3, MAPT/TAU, POLR2H/RPB8 and STUB1/CHIP, by recognizing backbone atoms of disordered N-termini (PubMed:23560854, PubMed:23696636, PubMed:25436519). Involved in degradation of misfolded chaperone substrates by mediating monoubiquitination of STUB1/CHIP, leading to recruitment of ATXN3 to monoubiquitinated STUB1/CHIP, and restriction of the length of ubiquitin chain attached to STUB1/CHIP substrates by ATXN3. After UV irradiation, but not after mitomycin-C (MMC) treatment, acts as a specific E2 ubiquitin-conjugating enzyme for the Fanconi anemia complex by associating with E3 ubiquitin-protein ligase FANCL and catalyzing monoubiquitination of FANCD2, a key step in the DNA damage pathway (PubMed:19111657, PubMed:21229326). In vitro catalyzes 'Lys-11'-linked polyubiquitination. UBE2W-catalyzed ubiquitination occurs also in the presence of inactive RING/U-box type E3s, i.e. lacking the active site cysteine residues to form thioester bonds with ubiquitin, or even in the absence of E3, albeit at a slower rate (PubMed:25436519). {ECO:0000269|PubMed:19111657, ECO:0000269|PubMed:20061386, ECO:0000269|PubMed:21229326, ECO:0000269|PubMed:23560854, ECO:0000269|PubMed:23696636, ECO:0000269|PubMed:25436519}.

Pfam Domains GO Terms

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
Citral 75μM R06 exp275	1.7
Mdivi-1 15μM R05 exp217	1.72
ABT-702 5μM plus Dimethyloxaloylglycine 11μM R07 exp319	1.75
Senexin-A 25μM R07 exp400	1.77
Dimethyloxaloylglycine 100μM R05 exp229	1.83
Prochlorperazine 5.2μM R02 exp77	1.85
CCT251545 20μM R07 exp326	1.9

Global Fraction of Cell Lines Where Essential: 0/739

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	0/1
909776.0	0/1
bile duct	0/28
blood	0/28
bone	0/26
breast	0/33
central nervous system	0/56
cervix	0/4
colorectal	0/17
esophagus	0/13
fibroblast	0/1
gastric	0/16
kidney	0/21
liver	0/20
lung	0/75
lymphocyte	0/16
ovary	0/26
pancreas	0/24
peripheral nervous system	0/16
plasma cell	0/15
prostate	0/1
skin	0/24
soft tissue	0/9
thyroid	0/2
upper aerodigestive	0/22
urinary tract	0/29
uterus	0/5

Expression Distribution

cellular response to misfolded protein
response to misfolded protein
protein quality control for misfolded or incompletely synthesized proteins
protein K11-linked ubiquitination
ubiquitin conjugating enzyme activity
protein monoubiquitination
cellular response to topologically incorrect protein
response to topologically incorrect protein
ubiquitin-protein transferase activity
ubiquitin protein ligase binding
protein polyubiquitination
proteasome-mediated ubiquitin-dependent protein catabolic process
proteasomal protein catabolic process
DNA repair
ubiquitin-dependent protein catabolic process
modification-dependent protein catabolic process
modification-dependent macromolecule catabolic process
proteolysis involved in cellular protein catabolic process
cellular protein catabolic process
protein catabolic process
protein ubiquitination
DNA metabolic process
protein modification by small protein conjugation
cellular response to DNA damage stimulus
cellular macromolecule catabolic process
protein modification by small protein conjugation or removal
macromolecule catabolic process
organonitrogen compound catabolic process
proteolysis
ATP binding
cellular response to stress
organic substance catabolic process
cellular catabolic process