E2F1

Gene Information

Official Symbol: E2F1
Official Name: E2F transcription factor 1
Aliases and Previous Symbols: N/A
Entrez ID: 1869
UniProt: Q01094
Interactions: BioGRID
PubMed articles: Open PubMed
OMIM: Open OMIM

Function Summary

Entrez Summary: The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis. [provided by RefSeq, Jul 2008].
UniProt Summary: Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC- 3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F1 binds preferentially RB1 in a cell-cycle dependent manner. It can mediate both cell proliferation and TP53/p53-dependent apoptosis. Blocks adipocyte differentiation by binding to specific promoters repressing CEBPA binding to its target gene promoters (PubMed:20176812). Positively regulates transcription of RRP1B (PubMed:20040599). {ECO:0000250|UniProtKB:Q61501, ECO:0000269|PubMed:10675335, ECO:0000269|PubMed:12717439, ECO:0000269|PubMed:17704056, ECO:0000269|PubMed:20040599, ECO:0000269|PubMed:20176812, ECO:0000269|PubMed:8170954}.

Pfam Domains GO Terms

CRISPR Data

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
SGC0946 7μM R03 exp151	-1.98
Aminopterin 0.005μM R07 exp335	1.78
Latrunculin-B 10μM R07 exp374	1.8
AICAR 240μM R05 exp211	1.85
Aphidicolin 0.4μM R08 exp440	1.91
Olaparib 4μM R08 exp512	2.11
Methotrexate 0.01μM R08 exp501	2.21
Trimetrexate 0.03μM R08 exp535	3.42

Global Fraction of Cell Lines Where Essential: 9/726

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	0/1
909776.0	0/1
bile duct	0/28
blood	0/28
bone	1/25
breast	1/33
central nervous system	1/56
cervix	0/4
colorectal	0/17
esophagus	0/13
fibroblast	0/1
gastric	0/15
kidney	0/21
liver	0/20
lung	3/75
lymphocyte	0/14
ovary	0/26
pancreas	1/24
peripheral nervous system	0/16
plasma cell	0/15
prostate	0/1
skin	0/24
soft tissue	0/7
thyroid	0/2
upper aerodigestive	1/22
urinary tract	0/29
uterus	0/5

Essentiality in NALM6

Essentiality Rank: 16567
Expression level (log2 read counts): 7.23

Expression Distribution

Rb-E2F complex
negative regulation of transcription involved in G1/S transition of mitotic cell cycle
lens fiber cell apoptotic process
negative regulation of fat cell proliferation
regulation of fat cell proliferation
anoikis
positive regulation of glial cell proliferation
regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway
positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway
epithelial cell apoptotic process
regulation of transcription involved in G1/S transition of mitotic cell cycle
regulation of glial cell proliferation
proximal promoter sequence-specific DNA binding
positive regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway
negative regulation of G0 to G1 transition
mRNA stabilization
regulation of G0 to G1 transition
regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway
negative regulation of fat cell differentiation
RNA stabilization
cellular response to nerve growth factor stimulus
intrinsic apoptotic signaling pathway by p53 class mediator
positive regulation of fibroblast proliferation
response to nerve growth factor
negative regulation of mRNA catabolic process
negative regulation of DNA binding
cellular response to fatty acid
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
intracellular signal transduction involved in G1 DNA damage checkpoint
signal transduction involved in mitotic DNA integrity checkpoint
signal transduction involved in mitotic cell cycle checkpoint
signal transduction involved in mitotic G1 DNA damage checkpoint
signal transduction involved in mitotic DNA damage checkpoint
positive regulation of establishment of protein localization to mitochondrion
negative regulation of RNA catabolic process
mitotic G1 DNA damage checkpoint
mitotic G1/S transition checkpoint
G1 DNA damage checkpoint
positive regulation of gliogenesis
intrinsic apoptotic signaling pathway in response to DNA damage