EXO5

Entrez Summary: N/A
UniProt Summary: Single-stranded DNA (ssDNA) bidirectional exonuclease involved in DNA repair. Probably involved in DNA repair following ultraviolet (UV) irradiation and interstrand cross-links (ICLs) damage. Has both 5'-3' and 3'-5' exonuclease activities with a strong preference for 5'-ends. Acts as a sliding exonuclease that loads at ssDNA ends and then slides along the ssDNA prior to cutting; however the sliding and the 3'-5' exonuclease activities are abolished upon binding to the replication protein A (RPA) complex that enforces 5'-directionality activity. {ECO:0000269|PubMed:23095756}.

Pfam Domains GO Terms

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
Palbociclib 1μM R07 exp382	-2.08
2-Methoxyestradiol 0.55 to 1μM on day4 R07 exp312	-1.83
Mdivi-1 15μM R05 exp217	-1.83
Geldanamycin 0.015 to 0.05μM on day4 R07 exp317	-1.81
Cyclosporin-A 0.8μM R07 exp347	-1.74
UM0131023 0.05μM R05 exp248	-1.72
BAY-598 4μM R05 exp220	-1.71
LJH685 5μM R02 exp73	1.7
Trametinib 10μM R02 exp83	1.79
Nocodazole 0.02μM R00 exp23	1.79

Global Fraction of Cell Lines Where Essential: 0/739

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	0/1
909776.0	0/1
bile duct	0/28
blood	0/28
bone	0/26
breast	0/33
central nervous system	0/56
cervix	0/4
colorectal	0/17
esophagus	0/13
fibroblast	0/1
gastric	0/16
kidney	0/21
liver	0/20
lung	0/75
lymphocyte	0/16
ovary	0/26
pancreas	0/24
peripheral nervous system	0/16
plasma cell	0/15
prostate	0/1
skin	0/24
soft tissue	0/9
thyroid	0/2
upper aerodigestive	0/22
urinary tract	0/29
uterus	0/5

Expression Distribution

single-stranded DNA 5-3 exodeoxyribonuclease activity
single-stranded DNA 3-5 exodeoxyribonuclease activity
4 iron, 4 sulfur cluster binding
interstrand cross-link repair
nucleic acid phosphodiester bond hydrolysis
DNA repair
DNA metabolic process
cellular response to DNA damage stimulus
protein homodimerization activity
DNA binding
cellular response to stress