GTF2H4

Gene Information

Official Symbol: GTF2H4
Official Name: general transcription factor IIH subunit 4
Aliases and Previous Symbols: N/A
Entrez ID: 2968
UniProt: Q92759
Interactions: BioGRID
PubMed articles: Open PubMed
OMIM: Open OMIM

Function Summary

Entrez Summary: N/A
UniProt Summary: Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre- initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription. {ECO:0000269|PubMed:9852112}.

Pfam Domains GO Terms

CRISPR Data

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
Geldanamycin 0.015 to 0.05μM on day4 R07 exp317	-1.89
Arsenate 40μM R06 exp264	-1.85
Hydroxyurea 100μM R07 exp329	-1.83
MK-1775 0.32μM R02 exp75	-1.77
BI-D1870 2μM R04 exp162	-1.74
Hinokiflavone 12μM R08 exp487	1.72
THZ1 0.06μM R08 exp531	1.72
Mubritinib 0.2μM R01 exp48	1.74
Menadione 5μM R06 exp292	1.89

Global Fraction of Cell Lines Where Essential: 341/726

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	0/1
909776.0	0/1
bile duct	15/28
blood	9/28
bone	8/25
breast	11/33
central nervous system	38/56
cervix	3/4
colorectal	12/17
esophagus	5/13
fibroblast	1/1
gastric	8/15
kidney	12/21
liver	7/20
lung	33/75
lymphocyte	7/14
ovary	12/26
pancreas	11/24
peripheral nervous system	4/16
plasma cell	11/15
prostate	1/1
skin	7/24
soft tissue	2/7
thyroid	2/2
upper aerodigestive	13/22
urinary tract	22/29
uterus	3/5

Essentiality in NALM6

Essentiality Rank: 1537
Expression level (log2 read counts): 4.95

Expression Distribution

Gene	Correlation
GTF2H3	0.428
CCNH	0.423

core TFIIH complex portion of holo TFIIH complex
phosphorylation of RNA polymerase II C-terminal domain
transcription factor TFIIH core complex
transcription factor TFIIH holo complex
RNA polymerase II general transcription initiation factor activity
RNA polymerase II CTD heptapeptide repeat kinase activity
nucleotide-excision repair, preincision complex stabilization
nucleotide-excision repair, DNA incision, 3-to lesion
nucleotide-excision repair, DNA duplex unwinding
ATPase activator activity
global genome nucleotide-excision repair
nucleotide-excision repair, preincision complex assembly
transcription elongation from RNA polymerase I promoter
termination of RNA polymerase I transcription
7-methylguanosine mRNA capping
7-methylguanosine RNA capping
RNA capping
transcription initiation from RNA polymerase I promoter
nucleotide-excision repair, DNA incision, 5-to lesion
transcription factor TFIID complex
transcription by RNA polymerase I
nucleotide-excision repair, DNA incision
DNA-dependent ATPase activity
positive regulation of ATPase activity
DNA-templated transcription, termination
transcription-coupled nucleotide-excision repair
transcription elongation from RNA polymerase II promoter
regulation of ATPase activity
DNA-templated transcription, elongation
double-stranded DNA binding
DNA duplex unwinding
nucleotide-excision repair
DNA geometric change
transcription initiation from RNA polymerase II promoter
protein-DNA complex assembly
DNA-templated transcription, initiation
protein kinase activity
protein-DNA complex subunit organization
positive regulation of protein complex assembly
DNA conformation change