SPHK2
Gene Information
- Official Symbol: SPHK2
- Official Name: sphingosine kinase 2
- Aliases and Previous Symbols: N/A
- Entrez ID: 56848
- UniProt: Q9NRA0
- Interactions: BioGRID
- PubMed articles: Open PubMed
- OMIM: Open OMIM
Function Summary
- Entrez Summary: This gene encodes one of two sphingosine kinase isozymes that catalyze the phosphorylation of sphingosine into sphingosine 1-phosphate. Sphingosine 1-phosphate mediates many cellular processes including migration, proliferation and apoptosis, and also plays a role in several types of cancer by promoting angiogenesis and tumorigenesis. The encoded protein may play a role in breast cancer proliferation and chemoresistance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2011].
- UniProt Summary: Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro- dihydrosphingosine, D-erythro-sphingosine and L-threo- dihydrosphingosine. Binds phosphoinositides. {ECO:0000269|PubMed:19168031}.
Pfam Domains GO Terms
Pfam Domains
| DAGK cat |
GO Terms
| histone H2B-K12 acetylation |
| sphinganine-1-phosphate biosynthetic process |
| interleukin-13 secretion |
| histone H2A-K5 acetylation |
| D-erythro-sphingosine kinase activity |
| sphinganine kinase activity |
| negative regulation of histone deacetylase activity |
| sphinganine-1-phosphate metabolic process |
| negative regulation of deacetylase activity |
| tumor necrosis factor secretion |
| histone H2B acetylation |
| interleukin-13 production |
| cytokine secretion involved in immune response |
| interleukin-6 secretion |
| nucleosomal histone binding |
| regulation of cytochrome-c oxidase activity |
| response to phorbol 13-acetate 12-myristate |
| cellular response to phorbol 13-acetate 12-myristate |
| negative regulation of histone deacetylation |
| regulation of electron transfer activity |
| tumor necrosis factor production |
| positive regulation of histone H3-K9 acetylation |
| sphingosine-1-phosphate receptor activity |
| regulation of histone deacetylase activity |
| tumor necrosis factor superfamily cytokine production |
| positive regulation of ceramide biosynthetic process |
| positive regulation of protein kinase C signaling |
| positive regulation of sphingolipid biosynthetic process |
| sphingosine-1-phosphate receptor signaling pathway |
| interleukin-6 production |
| regulation of deacetylase activity |
| sphingosine biosynthetic process |
| sphingolipid mediated signaling pathway |
| sphingoid biosynthetic process |
| sphingosine metabolic process |
| Ras GTPase binding |
| regulation of histone H3-K9 acetylation |
| positive regulation of mast cell activation involved in immune response |
| positive regulation of mast cell degranulation |
| regulation of protein kinase C signaling |
| regulation of sphingolipid biosynthetic process |
| regulation of membrane lipid metabolic process |
| regulation of ceramide biosynthetic process |
| sphingoid metabolic process |
| NAD+ kinase activity |
| histone H2A acetylation |
| regulation of ATP biosynthetic process |
| diol biosynthetic process |
| positive regulation of calcium ion import |
| positive regulation of mast cell activation |
| diol metabolic process |
| regulation of proton transport |
| cytokine production involved in immune response |
| positive regulation of leukocyte degranulation |
| regulation of histone deacetylation |
| positive regulation of histone acetylation |
| positive regulation of myeloid leukocyte mediated immunity |
| positive regulation of peptidyl-lysine acetylation |
| regulation of mast cell degranulation |
| regulation of mast cell activation involved in immune response |
| regulation of calcium ion import |
| regulation of purine nucleotide biosynthetic process |
| regulation of nucleotide biosynthetic process |
| negative regulation of histone modification |
| positive regulation of protein acetylation |
| regulation of protein deacetylation |
| regulation of mast cell activation |
| cytokine secretion |
| regulation of leukocyte degranulation |
| positive regulation of regulated secretory pathway |
| regulation of histone acetylation |
| regulation of myeloid leukocyte mediated immunity |
| polyol biosynthetic process |
| negative regulation of chromatin organization |
| regulation of peptidyl-lysine acetylation |
| positive regulation of gene expression, epigenetic |
| lipid phosphorylation |
| nucleosome |
| regulation of protein acetylation |
| positive regulation of lipid biosynthetic process |
| cellular response to alcohol |
| regulation of reactive oxygen species biosynthetic process |
| positive regulation of exocytosis |
| positive regulation of histone modification |
| sphingolipid biosynthetic process |
| cellular response to ketone |
| response to antineoplastic agent |
| regulation of oxidoreductase activity |
| positive regulation of chromatin organization |
| polyol metabolic process |
| alcohol biosynthetic process |
| histone acetylation |
| regulation of purine nucleotide metabolic process |
| regulation of nucleotide metabolic process |
| internal peptidyl-lysine acetylation |
| regulation of ATP metabolic process |
| peptidyl-lysine acetylation |
| positive regulation of calcium ion transport |
| internal protein amino acid acetylation |
| membrane lipid biosynthetic process |
| positive regulation of leukocyte mediated immunity |
| negative regulation of chromosome organization |
| positive regulation of lipid metabolic process |
| protein acetylation |
| regulation of histone modification |
| cytokine production |
| positive regulation of cellular amide metabolic process |
| sphingolipid metabolic process |
| protein secretion |
| regulation of generation of precursor metabolites and energy |
| establishment of protein localization to extracellular region |
| regulation of regulated secretory pathway |
| protein localization to extracellular region |
| peptide secretion |
| positive regulation of chromosome organization |
| regulation of reactive oxygen species metabolic process |
| production of molecular mediator of immune response |
| female pregnancy |
| negative regulation of cell growth |
| organic hydroxy compound biosynthetic process |
| regulation of lipid biosynthetic process |
| protein acylation |
| regulation of chromatin organization |
| ammonium ion metabolic process |
| response to ketone |
| lipid modification |
| membrane lipid metabolic process |
| regulation of leukocyte mediated immunity |
| positive regulation of intracellular transport |
| multi-multicellular organism process |
| positive regulation of immune effector process |
| regulation of exocytosis |
| response to alcohol |
| regulation of I-kappaB kinase/NF-kappaB signaling |
| regulation of gene expression, epigenetic |
| negative regulation of growth |
| phospholipid biosynthetic process |
| regulation of calcium ion transport |
| regulation of ion transmembrane transporter activity |
| regulation of transmembrane transporter activity |
| positive regulation of ion transport |
| regulation of transporter activity |
| lysosomal membrane |
| peptidyl-lysine modification |
| alcohol metabolic process |
| regulation of cation transmembrane transport |
| regulation of chromosome organization |
| regulation of intracellular transport |
| histone modification |
| negative regulation of organelle organization |
| phospholipid metabolic process |
| covalent chromatin modification |
| regulation of metal ion transport |
| mitochondrial inner membrane |
| regulation of lipid metabolic process |
| regulation of cellular amide metabolic process |
| positive regulation of secretion by cell |
| cellular response to drug |
| positive regulation of leukocyte activation |
| positive regulation of cell activation |
| regulation of cell growth |
| regulation of small molecule metabolic process |
| positive regulation of secretion |
| negative regulation of hydrolase activity |
| organic hydroxy compound metabolic process |
| regulation of immune effector process |
| regulation of ion transmembrane transport |
| blood vessel development |
| vasculature development |
| cardiovascular system development |
| cellular response to lipid |
| organophosphate biosynthetic process |
| regulation of vesicle-mediated transport |
| cell population proliferation |
| regulation of transmembrane transport |
| lipid biosynthetic process |
| small molecule biosynthetic process |
| negative regulation of protein modification process |
| regulation of leukocyte activation |
| positive regulation of organelle organization |
| regulation of cell activation |
| positive regulation of apoptotic process |
| positive regulation of programmed cell death |
| regulation of growth |
| intracellular membrane-bounded organelle |
| positive regulation of cell death |
| chromatin organization |
| regulation of ion transport |
| negative regulation of cellular component organization |
| brain development |
| regulation of secretion by cell |
| head development |
| negative regulation of catalytic activity |
| regulation of secretion |
| response to lipid |
| positive regulation of immune response |
| circulatory system development |
| peptidyl-amino acid modification |
| organophosphate metabolic process |
| negative regulation of apoptotic process |
| negative regulation of programmed cell death |
| positive regulation of cell population proliferation |
| regulation of cellular localization |
| cellular lipid metabolic process |
| regulation of cellular component biogenesis |
| central nervous system development |
| positive regulation of transport |
| negative regulation of cell death |
| multi-organism reproductive process |
| secretion by cell |
| endoplasmic reticulum |
| response to drug |
| positive regulation of intracellular signal transduction |
| negative regulation of cellular protein metabolic process |
| export from cell |
| cellular response to oxygen-containing compound |
| chromosome organization |
| negative regulation of protein metabolic process |
| secretion |
| negative regulation of molecular function |
| positive regulation of immune system process |
| regulation of immune response |
| positive regulation of cellular component organization |
| lipid metabolic process |
| positive regulation of protein modification process |
| mitochondrion |
| regulation of hydrolase activity |
| phosphorylation |
| regulation of organelle organization |
| G protein-coupled receptor signaling pathway |
| organonitrogen compound biosynthetic process |
| reproductive process |
| reproduction |
| ATP binding |
| protein transport |
| peptide transport |
| regulation of apoptotic process |
| response to oxygen-containing compound |
| regulation of programmed cell death |
| amide transport |
| positive regulation of cellular protein metabolic process |
| establishment of protein localization |
| regulation of cell population proliferation |
| cellular nitrogen compound biosynthetic process |
| regulation of immune system process |
| positive regulation of signal transduction |
| regulation of cell death |
| positive regulation of protein metabolic process |
| small molecule metabolic process |
| regulation of phosphate metabolic process |
| regulation of phosphorus metabolic process |
| positive regulation of cell communication |
| positive regulation of signaling |
| regulation of intracellular signal transduction |
| nitrogen compound transport |
| regulation of protein modification process |
| regulation of transport |
| immune response |
| positive regulation of cellular biosynthetic process |
| positive regulation of gene expression |
| membrane |
| positive regulation of biosynthetic process |
CRISPR Data
Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)
Compound Hit
| Screen | Score |
|---|---|
| Heregulin-B 44ng/ml R08 exp486 | 1.71 |
| FTY720 3μM R08 exp483 | 2.84 |
Most Correlated Genes in Chemogenomics
No correlation found to any other genes in chemogenomics.
Tissues where Essential in the Avana Dataset (DepMap 20Q1)
Global Fraction of Cell Lines Where Essential: 0/739
| Tissue | Fraction Of Cell Lines Where Essential |
|---|---|
| 1290807.0 | 0/1 |
| 909776.0 | 0/1 |
| bile duct | 0/28 |
| blood | 0/28 |
| bone | 0/26 |
| breast | 0/33 |
| central nervous system | 0/56 |
| cervix | 0/4 |
| colorectal | 0/17 |
| esophagus | 0/13 |
| fibroblast | 0/1 |
| gastric | 0/16 |
| kidney | 0/21 |
| liver | 0/20 |
| lung | 0/75 |
| lymphocyte | 0/16 |
| ovary | 0/26 |
| pancreas | 0/24 |
| peripheral nervous system | 0/16 |
| plasma cell | 0/15 |
| prostate | 0/1 |
| skin | 0/24 |
| soft tissue | 0/9 |
| thyroid | 0/2 |
| upper aerodigestive | 0/22 |
| urinary tract | 0/29 |
| uterus | 0/5 |
Essentiality in NALM6
- Essentiality Rank: 6085
- Expression level (log2 read counts): 4.73
Expression Distribution
SPHK2 Expression in NALM6 Cells: 4.73
