USP14

Gene Information

Official Symbol: USP14
Official Name: ubiquitin specific peptidase 14
Aliases and Previous Symbols: N/A
Entrez ID: 9097
UniProt: P54578
Interactions: BioGRID
PubMed articles: Open PubMed
OMIM: Open OMIM

Function Summary

Entrez Summary: N/A
UniProt Summary: Proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins. Ensures the regeneration of ubiquitin at the proteasome. Is a reversibly associated subunit of the proteasome and a large fraction of proteasome-free protein exists within the cell. Required for the degradation of the chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis. Serves also as a physiological inhibitor of endoplasmic reticulum-associated degradation (ERAD) under the non-stressed condition by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1. Indispensable for synaptic development and function at neuromuscular junctions (NMJs). Plays a role in the innate immune defense against viruses by stabilizing the viral DNA sensor CGAS and thus inhibiting its autophagic degradation. {ECO:0000269|PubMed:18162577, ECO:0000269|PubMed:19106094, ECO:0000269|PubMed:19135427, ECO:0000269|PubMed:27666593}.

Pfam Domains GO Terms

CRISPR Data

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
Tubastatin-A 2.5μM R07 exp417	-5.63
MK-1775 0.32μM R02 exp75	1.72
MS023 2μM R03 exp134	1.75
Nocodazole 0.2μM R02 exp64	1.75
Amiloride 100μM R08 exp447	1.86
Doxorubicin 0.02μM R08 exp478	1.93
Etoposide 1μM R00 exp19	1.95
UM0129480 7μM R02 exp85	1.95
2-Deoxy-D-glucose 800μM R05 exp214	1.98
Thimerosal 0.85μM R08 exp529	2.15
THZ531 0.11 to 0.125 to 0.35μM on day4 then day6 R07 exp412	2.27
GSK626616 14μM R08 exp485	2.34
Etoposide 0.1μM R05 exp198	2.35
CB-5083 0.4μM R08 exp468	2.7
Olaparib 4μM R08 exp512	2.87

Global Fraction of Cell Lines Where Essential: 5/739

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	0/1
909776.0	0/1
bile duct	0/28
blood	0/28
bone	0/26
breast	2/33
central nervous system	0/56
cervix	0/4
colorectal	0/17
esophagus	0/13
fibroblast	0/1
gastric	0/16
kidney	0/21
liver	0/20
lung	1/75
lymphocyte	0/16
ovary	1/26
pancreas	0/24
peripheral nervous system	0/16
plasma cell	0/15
prostate	0/1
skin	0/24
soft tissue	0/9
thyroid	0/2
upper aerodigestive	1/22
urinary tract	0/29
uterus	0/5

Essentiality in NALM6

Essentiality Rank: 13746
Expression level (log2 read counts): 7.17

Expression Distribution

tRNA guanylyltransferase activity
negative regulation of ER-associated ubiquitin-dependent protein catabolic process
regulation of ER-associated ubiquitin-dependent protein catabolic process
negative regulation of ERAD pathway
tRNA 5-end processing
proteasome binding
ncRNA 5-end processing
RNA 5-end processing
regulation of ERAD pathway
negative regulation of proteasomal ubiquitin-dependent protein catabolic process
endopeptidase inhibitor activity
negative regulation of response to endoplasmic reticulum stress
negative regulation of ubiquitin-dependent protein catabolic process
thiol-dependent ubiquitinyl hydrolase activity
proteasome complex
negative regulation of proteasomal protein catabolic process
negative regulation of proteolysis involved in cellular protein catabolic process
regulation of response to endoplasmic reticulum stress
negative regulation of cellular protein catabolic process
cysteine-type endopeptidase activity
thiol-dependent ubiquitin-specific protease activity
regulation of proteasomal ubiquitin-dependent protein catabolic process
tRNA processing
negative regulation of protein catabolic process
regulation of ubiquitin-dependent protein catabolic process
regulation of proteasomal protein catabolic process
tRNA metabolic process
regulation of chemotaxis
regulation of proteolysis involved in cellular protein catabolic process
negative regulation of endopeptidase activity
regulation of cellular protein catabolic process
cytoplasmic vesicle
negative regulation of cellular catabolic process
negative regulation of peptidase activity
synapse
protein deubiquitination
protein modification by small protein removal
negative regulation of catabolic process
negative regulation of proteolysis
regulation of protein catabolic process