USP33

Gene Information

Official Symbol: USP33
Official Name: ubiquitin specific peptidase 33
Aliases and Previous Symbols: N/A
Entrez ID: 23032
UniProt: Q8TEY7
Interactions: BioGRID
PubMed articles: Open PubMed
OMIM: Open OMIM

Function Summary

Entrez Summary: N/A
UniProt Summary: Deubiquitinating enzyme involved in various processes such as centrosome duplication, cellular migration and beta-2 adrenergic receptor/ADRB2 recycling. Involved in regulation of centrosome duplication by mediating deubiquitination of CCP110 in S and G2/M phase, leading to stabilize CCP110 during the period which centrioles duplicate and elongate. Involved in cell migration via its interaction with intracellular domain of ROBO1, leading to regulate the Slit signaling. Plays a role in commissural axon guidance cross the ventral midline of the neural tube in a Slit-dependent manner, possibly by mediating the deubiquitination of ROBO1. Acts as a regulator of G-protein coupled receptor (GPCR) signaling by mediating the deubiquitination of beta-arrestins (ARRB1 and ARRB2) and beta-2 adrenergic receptor (ADRB2). Plays a central role in ADRB2 recycling and resensitization after prolonged agonist stimulation by constitutively binding ADRB2, mediating deubiquitination of ADRB2 and inhibiting lysosomal trafficking of ADRB2. Upon dissociation, it is probably transferred to the translocated beta- arrestins, leading to beta-arrestins deubiquitination and disengagement from ADRB2. This suggests the existence of a dynamic exchange between the ADRB2 and beta-arrestins. Deubiquitinates DIO2, thereby regulating thyroid hormone regulation. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. {ECO:0000269|PubMed:12865408, ECO:0000269|PubMed:19363159, ECO:0000269|PubMed:19424180, ECO:0000269|PubMed:23486064}.

Pfam Domains GO Terms

CRISPR Data

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
Trametinib 10μM R02 exp83	-2.01
Olaparib 4μM R08 exp512	-1.93
A-395 10μM R05 exp218	-1.8
All-trans-Retinoic-Acid 40μM R07 exp334	1.74
Nocodazole 0.02μM R00 exp23	1.76
Methotrexate 0.01μM R08 exp501	1.88

Global Fraction of Cell Lines Where Essential: 0/739

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	0/1
909776.0	0/1
bile duct	0/28
blood	0/28
bone	0/26
breast	0/33
central nervous system	0/56
cervix	0/4
colorectal	0/17
esophagus	0/13
fibroblast	0/1
gastric	0/16
kidney	0/21
liver	0/20
lung	0/75
lymphocyte	0/16
ovary	0/26
pancreas	0/24
peripheral nervous system	0/16
plasma cell	0/15
prostate	0/1
skin	0/24
soft tissue	0/9
thyroid	0/2
upper aerodigestive	0/22
urinary tract	0/29
uterus	0/5

Essentiality in NALM6

Essentiality Rank: 15476
Expression level (log2 read counts): 6.24

Expression Distribution

zf-UBP
DUSP
UCH

VCB complex
Ral GTPase binding
centrosome duplication
protein K63-linked deubiquitination
protein K48-linked deubiquitination
thiol-dependent ubiquitinyl hydrolase activity
G protein-coupled receptor binding
cell body
ubiquitin binding
centrosome cycle
cysteine-type endopeptidase activity
microtubule organizing center organization
positive regulation of protein binding
negative regulation of protein binding
thiol-dependent ubiquitin-specific protease activity
regulation of G protein-coupled receptor signaling pathway
cellular response to starvation
negative regulation of binding
protein stabilization
positive regulation of binding
response to starvation
regulation of protein binding
cellular response to nutrient levels
axon guidance
neuron projection guidance
cellular response to extracellular stimulus
protein deubiquitination
regulation of protein stability
protein modification by small protein removal
regulation of autophagy
cellular response to external stimulus
axonogenesis
regulation of binding
axon development
focal adhesion
cell morphogenesis involved in neuron differentiation
microtubule cytoskeleton organization
neuron projection morphogenesis
plasma membrane bounded cell projection morphogenesis
cell projection morphogenesis