USP4

Gene Information

Official Symbol: USP4
Official Name: ubiquitin specific peptidase 4
Aliases and Previous Symbols: N/A
Entrez ID: 7375
UniProt: Q13107
Interactions: BioGRID
PubMed articles: Open PubMed
OMIM: Open OMIM

Function Summary

Entrez Summary: The protein encoded by this gene is a protease that deubiquitinates target proteins such as ADORA2A and TRIM21. The encoded protein shuttles between the nucleus and cytoplasm and is involved in maintaining operational fidelity in the endoplasmic reticulum. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011].
UniProt Summary: Hydrolase that deubiquitinates target proteins such as the receptor ADORA2A, PDPK1 and TRIM21. Deubiquitination of ADORA2A increases the amount of functional receptor at the cell surface. May regulate mRNA splicing through deubiquitination of the U4 spliceosomal protein PRPF3. This may prevent its recognition by the U5 component PRPF8 thereby destabilizing interactions within the U4/U6.U5 snRNP. May also play a role in the regulation of quality control in the ER. {ECO:0000269|PubMed:16316627, ECO:0000269|PubMed:16339847, ECO:0000269|PubMed:16472766, ECO:0000269|PubMed:20595234, ECO:0000269|PubMed:7784062}.

Pfam Domains GO Terms

CRISPR Data

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
Bortezomib 0.05μM R00 exp7	-1.79
Phenformin 20μM R03 exp143	-1.71
Cholesterol 0.003 to 0.006 to 0.1μM on day2 then day6 R08 exp471	1.7
BS-181 20μM R08 exp461	1.76
Pterostilbene 16μM R02 exp78	1.81

Global Fraction of Cell Lines Where Essential: 1/739

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	0/1
909776.0	0/1
bile duct	0/28
blood	0/28
bone	0/26
breast	0/33
central nervous system	0/56
cervix	0/4
colorectal	0/17
esophagus	0/13
fibroblast	0/1
gastric	0/16
kidney	0/21
liver	0/20
lung	1/75
lymphocyte	0/16
ovary	0/26
pancreas	0/24
peripheral nervous system	0/16
plasma cell	0/15
prostate	0/1
skin	0/24
soft tissue	0/9
thyroid	0/2
upper aerodigestive	0/22
urinary tract	0/29
uterus	0/5

Essentiality in NALM6

Essentiality Rank: 16090
Expression level (log2 read counts): 6.29

Expression Distribution

DUSP
UCH

adenosine receptor binding
spliceosomal tri-snRNP complex assembly
protein localization to cell surface
spliceosomal snRNP assembly
thiol-dependent ubiquitinyl hydrolase activity
negative regulation of protein ubiquitination
negative regulation of protein modification by small protein conjugation or removal
thiol-dependent ubiquitin-specific protease activity
regulation of protein ubiquitination
regulation of protein modification by small protein conjugation or removal
ribonucleoprotein complex assembly
ribonucleoprotein complex subunit organization
lysosome
protein deubiquitination
regulation of protein stability
RNA splicing, via transesterification reactions with bulged adenosine as nucleophile
protein modification by small protein removal
mRNA splicing, via spliceosome
RNA splicing, via transesterification reactions
RNA splicing
ribonucleoprotein complex biogenesis
mRNA processing
ubiquitin-dependent protein catabolic process
modification-dependent protein catabolic process
modification-dependent macromolecule catabolic process
proteolysis involved in cellular protein catabolic process
negative regulation of protein modification process
cellular protein catabolic process
protein catabolic process
mRNA metabolic process
cellular protein-containing complex assembly
RNA processing
cellular macromolecule catabolic process
protein modification by small protein conjugation or removal
negative regulation of cellular protein metabolic process
macromolecule catabolic process
organonitrogen compound catabolic process
identical protein binding
negative regulation of protein metabolic process
proteolysis