VPS4B

Gene Information

Official Symbol: VPS4B
Official Name: vacuolar protein sorting 4 homolog B
Aliases and Previous Symbols: N/A
Entrez ID: 9525
UniProt: O75351
Interactions: BioGRID
PubMed articles: Open PubMed
OMIM: Open OMIM

Function Summary

Entrez Summary: N/A
UniProt Summary: Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses). VPS4A/B are required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413). {ECO:0000269|PubMed:11563910, ECO:0000269|PubMed:14505570, ECO:0000269|PubMed:18687924, ECO:0000269|PubMed:22660413}.

Pfam Domains GO Terms

CRISPR Data

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Global Fraction of Cell Lines Where Essential: 32/739

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	0/1
909776.0	0/1
bile duct	3/28
blood	0/28
bone	0/26
breast	2/33
central nervous system	0/56
cervix	0/4
colorectal	3/17
esophagus	0/13
fibroblast	0/1
gastric	1/16
kidney	0/21
liver	0/20
lung	2/75
lymphocyte	0/16
ovary	1/26
pancreas	2/24
peripheral nervous system	0/16
plasma cell	1/15
prostate	0/1
skin	0/24
soft tissue	0/9
thyroid	0/2
upper aerodigestive	3/22
urinary tract	1/29
uterus	1/5

Essentiality in NALM6

Essentiality Rank: 16138
Expression level (log2 read counts): 5.99

Expression Distribution

Screen	Score
DKK1 89ng/ml R08 exp477	-1.94
Lead acetate 2000μM R08 exp498 no dilution day6	-1.77
Pimelic-diphenylamide-106 0.5μM R00 exp27	1.81

Vps4 C
AAA
MIT

late endosomal microautophagy
negative regulation of exosomal secretion
positive regulation of centriole elongation
regulation of centriole elongation
ubiquitin-independent protein catabolic process via the multivesicular body sorting pathway
exit from host
viral release from host cell
positive regulation of centriole replication
movement in host environment
movement in environment of other organism involved in symbiotic interaction
exit from host cell
endosome to lysosome transport via multivesicular body sorting pathway
positive regulation of centrosome duplication
ESCRT III complex disassembly
ESCRT complex disassembly
positive regulation of centrosome cycle
positive regulation of viral release from host cell
positive regulation of exosomal secretion
midbody abscission
regulation of exosomal secretion
ATPase activity, coupled
viral budding via host ESCRT complex
mitotic cytokinetic process
ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway
regulation of mitotic spindle assembly
regulation of centriole replication
viral budding
endosome transport via multivesicular body sorting pathway
positive regulation of G2/M transition of mitotic cell cycle
Flemming body
regulation of spindle assembly
positive regulation of cell cycle G2/M phase transition
multivesicular body assembly
regulation of viral release from host cell
multivesicular body organization
negative regulation of exocytosis
multivesicular body sorting pathway
cytokinetic process
protein depolymerization
virion assembly