RPA1

Gene Information

Official Symbol: RPA1
Official Name: replication protein A1
Aliases and Previous Symbols: N/A
Entrez ID: 6117
UniProt: P27694
Interactions: BioGRID
PubMed articles: Open PubMed
OMIM: Open OMIM

Function Summary

Entrez Summary: N/A
UniProt Summary: As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism (PubMed:27723720, PubMed:27723717). Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage (PubMed:9430682). In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response (PubMed:24332808). It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage (PubMed:17765923). Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair (PubMed:7697716). Plays also a role in base excision repair (BER) probably through interaction with UNG (PubMed:9765279). Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance (PubMed:17959650). As part of the alternative replication protein A complex, aRPA, binds single- stranded DNA and probably plays a role in DNA repair. Compared to the RPA2-containing, canonical RPA complex, may not support chromosomal DNA replication and cell cycle progression through S- phase. The aRPA may not promote efficient priming by DNA polymerase alpha but could support DNA synthesis by polymerase delta in presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange (PubMed:19996105). {ECO:0000269|PubMed:12791985, ECO:0000269|PubMed:17765923, ECO:0000269|PubMed:17959650, ECO:0000269|PubMed:19116208, ECO:0000269|PubMed:19996105, ECO:0000269|PubMed:24332808, ECO:0000269|PubMed:27723717, ECO:0000269|PubMed:27723720, ECO:0000269|PubMed:7697716, ECO:0000269|PubMed:7700386, ECO:0000269|PubMed:9430682, ECO:0000269|PubMed:9765279}.

Pfam Domains GO Terms

CRISPR Data

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
Aminopterin 0.005μM R07 exp335	-1.85
Temozolomide 200μM R04 exp189	-1.79
Nocodazole 0.2μM R00 exp24	-1.7
Isoniazid 100μM R08 exp494	1.73
IL-3 9ng/ml R08 exp493	1.74
UM0130462 0.025 to 0.035μM day4 R05 exp247	1.81
SB203580 25μM R03 exp149	1.83
BI-D1870 2μM R04 exp162	1.87
Aphidicolin 0.04μM R06 exp263	1.88
Citral 50μM R06 exp274	1.89
Pyronaridine 1μM R06 exp295	1.93
Cisplatin 0.35μM R06 exp273	1.94
NN-Dimethylsphingosine 2.5μM R08 exp508	1.96
Vinorelbine 0.001μM R05 exp249	2.05
Nicotinamide Riboside 100μM R03 exp139	2.08
Alpha-Amanitin 0.5μM R00 exp5	2.13
39°C R06 exp303	2.21
D-Fructose 10000μM R06 exp279	2.25
VER-155008 3.9μM R06 exp301	2.25
Dihydrosphingosine 8μM R08 exp476	3.17

Gene	Correlation
HGC6.3	0.627
PRIM1	0.503

Global Fraction of Cell Lines Where Essential: 735/739

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	1/1
909776.0	1/1
bile duct	27/28
blood	28/28
bone	26/26
breast	33/33
central nervous system	55/56
cervix	4/4
colorectal	17/17
esophagus	13/13
fibroblast	1/1
gastric	16/16
kidney	20/21
liver	20/20
lung	75/75
lymphocyte	16/16
ovary	26/26
pancreas	24/24
peripheral nervous system	16/16
plasma cell	14/15
prostate	1/1
skin	24/24
soft tissue	9/9
thyroid	2/2
upper aerodigestive	22/22
urinary tract	29/29
uterus	5/5

Essentiality in NALM6

Essentiality Rank: 186
Expression level (log2 read counts): 7.68

Expression Distribution