POLD3

Gene Information

Official Symbol: POLD3
Official Name: DNA polymerase delta 3, accessory subunit
Aliases and Previous Symbols: N/A
Entrez ID: 10714
UniProt: Q15054
Interactions: BioGRID
PubMed articles: Open PubMed
OMIM: Open OMIM

Function Summary

Entrez Summary: N/A
UniProt Summary: As a component of the trimeric and tetrameric DNA polymerase delta complexes (Pol-delta3 and Pol-delta4, respectively), plays a role in high fidelity genome replication, including in lagging strand synthesis, and repair. Required for optimal Pol-delta activity. Stabilizes the Pol-delta complex and plays a major role in Pol-delta stimulation by PCNA (PubMed:10219083, PubMed:10852724, PubMed:11595739, PubMed:16510448, PubMed:24035200). Pol-delta3 and Pol-delta4 are characterized by the absence or the presence of POLD4. They exhibit differences in catalytic activity. Most notably, Pol- delta3 shows higher proofreading activity than Pol-delta4 (PubMed:19074196, PubMed:20334433). Although both Pol-delta3 and Pol-delta4 process Okazaki fragments in vitro, Pol-delta3 may also be better suited to fulfill this task, exhibiting near-absence of strand displacement activity compared to Pol-delta4 and stalling on encounter with the 5'-blocking oligonucleotides. Pol-delta3 idling process may avoid the formation of a gap, while maintaining a nick that can be readily ligated (PubMed:24035200). Along with DNA polymerase kappa, DNA polymerase delta carries out approximately half of nucleotide excision repair (NER) synthesis following UV irradiation. In this context, POLD3, along with PCNA and RFC1-replication factor C complex, is required to recruit POLD1, the catalytic subunit of the polymerase delta complex, to DNA damage sites (PubMed:20227374). Under conditions of DNA replication stress, required for the repair of broken replication forks through break-induced replication (BIR) (PubMed:24310611). Involved in the translesion synthesis (TLS) of templates carrying O6-methylguanine or abasic sites performed by Pol-delta4, independently of DNA polymerase zeta (REV3L) or eta (POLH). Facilitates abasic site bypass by DNA polymerase delta by promoting extension from the nucleotide inserted opposite the lesion (PubMed:19074196, PubMed:25628356, PubMed:27185888). Also involved in TLS, as a component of the POLZ complex. Along with POLD2, dramatically increases the efficiency and processivity of DNA synthesis of the minimal DNA polymerase zeta complex, consisting of only REV3L and REV7 (PubMed:24449906). {ECO:0000269|PubMed:10219083, ECO:0000269|PubMed:10852724, ECO:0000269|PubMed:11595739, ECO:0000269|PubMed:16510448, ECO:0000269|PubMed:19074196, ECO:0000269|PubMed:20227374, ECO:0000269|PubMed:20334433, ECO:0000269|PubMed:24035200, ECO:0000269|PubMed:24310611, ECO:0000269|PubMed:24449906, ECO:0000269|PubMed:25628356, ECO:0000269|PubMed:27185888}.

Pfam Domains GO Terms

CRISPR Data

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
Tanespimycin 14μM R08 exp527	-2.39
SNS-032 15μM R08 exp443	-2.36
Rapamycin 0.001μM R08 exp429	-2.13
TIC10 10μM R08 exp532	-2.12
MS023 7μM R03 exp135	-2.06
Vitamin-D3 40μM R08 exp536	-1.95
UM0129480 7μM R02 exp85	-1.94
Vinblastine 0.015μM R05 exp208	-1.92
Etoposide 1μM R00 exp19	-1.91
Dinaciclib 0.007μM R07 exp355	-1.86
CB-5083 0.4μM R08 exp468	-1.81
Oligomycin-A 20μM R00 exp26	-1.81
Cannabidiol 13μM R08 exp465	-1.73
Pterostilbene 16μM R02 exp78	-1.71
THZ1 0.06μM R08 exp531	-1.71
Trichostatin-A 0.06μM R07 exp415	1.78
BI-2536 0.0042μM R03 exp95	1.79
NFN1 0.1μM R01 exp49	1.95
S-trityl-L-cysteine 0.5μM R05 exp243	1.96
GSK343 3μM R03 exp124	2.09
UNC2400 2μM R03 exp156	2.14
GSK461364A 0.005μM R03 exp125	2.2
Apcin 50 to 100μM on day4 R04 exp176	2.22
A-196 10μM R03 exp114	2.27
Dimethyl-Sulfoxide 1pc R03 exp120	2.35
FBS-Wisent 0.1 R07 exp426	2.46

Gene	Correlation
CBWD5	0.414
CBWD3	0.406
TCEB3CL2	0.4

Global Fraction of Cell Lines Where Essential: 735/739

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	1/1
909776.0	1/1
bile duct	28/28
blood	28/28
bone	26/26
breast	33/33
central nervous system	56/56
cervix	3/4
colorectal	17/17
esophagus	13/13
fibroblast	1/1
gastric	15/16
kidney	20/21
liver	20/20
lung	75/75
lymphocyte	16/16
ovary	26/26
pancreas	24/24
peripheral nervous system	16/16
plasma cell	15/15
prostate	1/1
skin	24/24
soft tissue	9/9
thyroid	2/2
upper aerodigestive	22/22
urinary tract	29/29
uterus	5/5

Essentiality in NALM6

Essentiality Rank: 1380
Expression level (log2 read counts): 6.3

Expression Distribution