USP8

Gene Information

Official Symbol: USP8
Official Name: ubiquitin specific peptidase 8
Aliases and Previous Symbols: N/A
Entrez ID: 9101
UniProt: P40818
Interactions: BioGRID
PubMed articles: Open PubMed
OMIM: Open OMIM

Function Summary

Entrez Summary: This gene encodes a protein that belongs to the ubiquitin-specific processing protease family of proteins. The encoded protein is thought to regulate the morphology of the endosome by ubiquitination of proteins on this organelle and is involved in cargo sorting and membrane trafficking at the early endosome stage. This protein is required for the cell to enter the S phase of the cell cycle and also functions as a positive regulator in the Hedgehog signaling pathway in development. Pseudogenes of this gene are present on chromosomes 2 and 6. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013].
UniProt Summary: Hydrolase that can remove conjugated ubiquitin from proteins and therefore plays an important regulatory role at the level of protein turnover by preventing degradation. Converts both 'Lys-48' an 'Lys-63'-linked ubiquitin chains. Catalytic activity is enhanced in the M phase. Involved in cell proliferation. Required to enter into S phase in response to serum stimulation. May regulate T-cell anergy mediated by RNF128 via the formation of a complex containing RNF128 and OTUB1. Probably regulates the stability of STAM2 and RASGRF1. Regulates endosomal ubiquitin dynamics, cargo sorting, membrane traffic at early endosomes, and maintenance of ESCRT-0 stability. The level of protein ubiquitination on endosomes is essential for maintaining the morphology of the organelle. Deubiquitinates EPS15 and controles tyrosine kinase stability. Removes conjugated ubiquitin from EGFR thus regulating EGFR degradation and downstream MAPK signaling. Involved in acrosome biogenesis through interaction with the spermatid ESCRT-0 complex and microtubules. Deubiquitinates BIRC6/bruce and KIF23/MKLP1. {ECO:0000269|PubMed:16520378, ECO:0000269|PubMed:17711858, ECO:0000269|PubMed:18329369, ECO:0000269|PubMed:9628861}.

Pfam Domains GO Terms

CRISPR Data

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
42°C R08 exp539	-2.51
WYE-354 6μM R02 exp90	-2.29
BVD-523 15μM R02 exp67	-2.19
Vemurafenib 6.6μM R02 exp89	-2.17
Pterostilbene 16μM R02 exp78	-2.16
Hydroxyurea 15μM R06 exp289	-2.12
Nocodazole 0.2μM R02 exp64	-2.01
BI-D1870 3.15μM R02 exp66	-1.95
LLY-283 2.6μM R06 exp290	-1.89
Rotenone 2μM R00 exp33	-1.88
Benzoate 20000μM R08 exp456	-1.81
2-Methoxyestradiol 0.55 to 0.75μM on day4 R07 exp311	-1.79
DABN 20μM R00 exp17	-1.78
GSK2606414 1μM R05 exp236	-1.74
PU-H71 1μM R08 exp515	-1.73
Fluvastatin 2.2μM R06 exp282	-1.71
SGC2096 2.6μM R03 exp153	1.71

Gene	Correlation
POLR2J3	0.422

Global Fraction of Cell Lines Where Essential: 286/739

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	0/1
909776.0	0/1
bile duct	7/28
blood	16/28
bone	6/26
breast	17/33
central nervous system	14/56
cervix	0/4
colorectal	7/17
esophagus	4/13
fibroblast	0/1
gastric	7/16
kidney	7/21
liver	8/20
lung	34/75
lymphocyte	8/16
ovary	13/26
pancreas	7/24
peripheral nervous system	2/16
plasma cell	10/15
prostate	1/1
skin	12/24
soft tissue	4/9
thyroid	1/2
upper aerodigestive	9/22
urinary tract	14/29
uterus	3/5

Essentiality in NALM6

Essentiality Rank: 1958
Expression level (log2 read counts): 7.08

Expression Distribution