Table of Contents

4-Nitroquinoline-1-oxide 0.5μM R08 exp446

4-Nitroquinoline-1-oxide 0.5μM R08 exp446

Mechanism of Action

UV mimetic, metabolized to electrohpile that forms stable quinolone monoadducts

Class / Subclass 1: DNA Damage, Repair and Replication / Helix-Distorting Adduct

Technical Notes

Protein References

PubChem Name: 4-Nitroquinoline N-oxide
Synonyms: N/A
CAS #: 56-57-5
PubChem CID: 5955
IUPAC: 4-nitro-1-oxidoquinolin-1-ium
INCHI Name: InChI=1S/C9H6N2O3/c12-10-6-5-9(11(13)14)7-3-1-2-4-8(7)10/h1-6H
INCHI Key: YHQDZJICGQWFHK-UHFFFAOYSA-N
Molecular Weight: 190.16
Canonical SMILES: C1=CC=C2C(=C1)C(=CC=[N+]2[O-])[N+](=O)[O-]
Isomeric SMILES: N/A
Molecular Formula: C9H6N2O3

Protein Supplier

Supplier Name: Enamine
Catalog #: N300-100463
Lot #: R284185

Protein Characterization

HRMS (ESI-TOF) m/z: (M+H)+ Calcd for C9H6N2O3 191.04512; found 191.04473

Dose Response Curve

Platform ID: 4NQO
Min: -1.1393; Max: 99.8617

IC	Concentration (µM)
IC10	0.2569
IC20	0.3742
IC30	0.4805
IC40	0.5898
IC50	0.7118
IC60	0.8591
IC70	1.0550
IC80	1.3540
IC90	1.9730

Screen Summary

Round: 08
Dose: 500nM
Days of incubation: 8
Doublings: 3.8
Numbers of reads: 19213659

Screen Results

Sensitive/Resistant hits (FDR<0.05)	CRANKS	Score Plot	Top 30 Genes	Screen Similarity	Top 30 Sensitive GO terms	Top 30 Resistant GO terms
6/3	Scores	View	View	View	View	View

Gene	CRANKS Score	FDR
ERCC6	-3.66	<0.01
UVSSA	-3.42	<0.01
CCDC111	-3.23	<0.01
ERCC8	-3.23	<0.01
SCAI	-3.17	<0.01
GTPBP2	-2.70	0.02
NRAP	-2.50	0.08
WDR77	-2.47	0.18
QRICH1	-2.42	0.08
CHD8	-2.40	0.08
UBE2A	-2.35	0.08
XPC	-2.35	0.08
POLR1B	-2.32	0.61
TUBGCP3	-2.31	0.10
FSBP	-2.29	0.19
VPS25	-2.22	0.54
DNAJC9	-2.20	0.17
GTF2H5	-2.12	0.22
SBNO1	-2.08	0.47
SIVA1	-2.06	0.29
KPNA3	-2.05	0.40
LIPH	-2.05	0.29
PARK7	-2.04	0.29
MCM9	-2.03	0.42
PLEKHA2	-2.03	0.45
UBA3	-2.02	0.46
NME5	-2.02	0.30
TRMT12	-2.01	0.30
UHRF1	-2.01	0.46
REV1	-2.01	0.30
AHCYL1	1.91	0.60
RPAP1	1.91	0.76
MTRNR2L3	1.92	0.76
SNX27	1.92	0.48
MAPK6	1.95	0.45
CRABP2	1.95	0.45
SHMT2	1.96	0.68
RHOH	1.96	0.54
KIAA0930	1.99	0.40
RBM19	2.06	0.68
CILP2	2.08	0.28
ODF4	2.11	0.40
ATPIF1	2.12	0.40
RPL11	2.17	0.68
SNIP1	2.20	0.45
PPP5C	2.20	0.20
TMEM17	2.21	0.20
DLEC1	2.24	0.26
CDK13	2.24	0.26
ZNF224	2.25	0.19
MAPKAP1	2.25	0.26
BDP1	2.29	0.19
BMP5	2.32	0.20
COQ7	2.35	0.16
ZNF330	2.39	0.19
BAK1	2.45	0.12
RPL5	2.92	0.15
NADK	3.43	<0.01
SLFN11	4.30	<0.01
NMRAL1	4.90	<0.01

GO Term	Fold Change	Genes
transcription elongation by RNA polymerase I	394.50	ERCC6,GTF2H5
double-strand break repair via classical nonhomologous end joining	236.70	ERCC6,ERCC8
transcription-coupled nucleotide-excision repair	197.25	ERCC6,UVSSA,ERCC8
rRNA transcription	84.54	ERCC6,POLR1B,GTF2H5
transcription by RNA polymerase I	65.75	ERCC6,POLR1B,GTF2H5
nucleotide-excision repair	51.01	ERCC6,UVSSA,ERCC8,XPC,GTF2H5
small molecule sensor activity	47.98	XPC,PARK7,UHRF1
response to UV	24.15	ERCC6,UVSSA,PRIMPOL,ERCC8,UBE2A,XPC
DNA repair	12.62	ERCC6,UVSSA,PRIMPOL,ERCC8,UBE2A,XPC,GTF2H5,PARK7,MCM9,UHRF1
response to light stimulus	11.72	ERCC6,UVSSA,PRIMPOL,ERCC8,UBE2A,XPC
response to radiation	10.13	ERCC6,UVSSA,PRIMPOL,ERCC8,UBE2A,XPC,GTF2H5
DNA damage response	8.31	ERCC6,UVSSA,PRIMPOL,ERCC8,UBE2A,XPC,GTF2H5,PARK7,MCM9,UHRF1
DNA metabolic process	8.29	ERCC6,UVSSA,PRIMPOL,ERCC8,UBE2A,XPC,GTF2H5,PARK7,MCM9,UHRF1