RFFL

Gene Information

Official Symbol: RFFL
Official Name: ring finger and FYVE like domain containing E3 ubiquitin protein ligase
Aliases and Previous Symbols: N/A
Entrez ID: 117584
UniProt: Q8WZ73
Interactions: BioGRID
PubMed articles: Open PubMed
OMIM: Open OMIM

Function Summary

Entrez Summary: N/A
UniProt Summary: E3 ubiquitin-protein ligase that regulates several biological processes through the ubiquitin-mediated proteasomal degradation of various target proteins. Mediates 'Lys-48'-linked polyubiquitination of PRR5L and its subsequent proteasomal degradation thereby indirectly regulating cell migration through the mTORC2 complex. Ubiquitinates the caspases CASP8 and CASP10, promoting their proteasomal degradation, to negatively regulate cell death downstream of death domain receptors in the extrinsic pathway of apoptosis. Negatively regulates the tumor necrosis factor-mediated signaling pathway through targeting of RIPK1 to ubiquitin-mediated proteasomal degradation. Negatively regulates p53/TP53 through its direct ubiquitination and targeting to proteasomal degradation. Indirectly, may also negatively regulate p53/TP53 through ubiquitination and degradation of SFN. May also play a role in endocytic recycling. {ECO:0000269|PubMed:15069192, ECO:0000269|PubMed:17121812, ECO:0000269|PubMed:18382127, ECO:0000269|PubMed:18450452, ECO:0000269|PubMed:22609986}.

Pfam Domains GO Terms

CRISPR Data

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Gene	Correlation
TCEB3CL2	0.472
TCEB3C	0.469
PCF11	0.461
AKIRIN2	0.461
C14orf93	0.448
TCEB3CL	0.447
RBBP5	0.437
NOP16	0.432
CNOT1	0.431
NPIPB5	0.426
AAMP	0.422
SLC6A17	0.42
SF3A3	0.417
NOL10	0.416
ANKLE1	0.415
RPL30	0.414
RPS28	0.413
RPS12	0.412
IK	0.411
LRR1	0.411
SNIP1	0.41
KCTD17	0.41
WDR87	0.41
ZNF830	0.409
MYO1H	0.409
PARP3	0.409
CHD4	0.408
ORC1	0.407
TINF2	0.406
SUPT6H	0.404
TBC1D3H	0.404
NUP160	0.404
EIF4A3	0.402
COPE	0.4

Global Fraction of Cell Lines Where Essential: 3/739

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	0/1
909776.0	0/1
bile duct	1/28
blood	0/28
bone	0/26
breast	0/33
central nervous system	0/56
cervix	0/4
colorectal	0/17
esophagus	0/13
fibroblast	0/1
gastric	0/16
kidney	0/21
liver	0/20
lung	0/75
lymphocyte	0/16
ovary	0/26
pancreas	0/24
peripheral nervous system	0/16
plasma cell	0/15
prostate	0/1
skin	0/24
soft tissue	0/9
thyroid	0/2
upper aerodigestive	0/22
urinary tract	0/29
uterus	0/5

Essentiality in NALM6

Essentiality Rank: 1874
Expression level (log2 read counts): 4.62

Expression Distribution

Screen	Score
Doxorubicin 0.02μM R08 exp478	-1.82
Epothilone-D 0.004 to 0.006μM on day4 R05 exp232	1.74
Pimelic-diphenylamide-106 0.5μM R00 exp27	2.19

tumor necrosis factor receptor superfamily complex
negative regulation of cysteine-type endopeptidase activity involved in execution phase of apoptosis
regulation of cysteine-type endopeptidase activity involved in execution phase of apoptosis
negative regulation of tumor necrosis factor-mediated signaling pathway
negative regulation of execution phase of apoptosis
regulation of fibroblast migration
negative regulation of signal transduction by p53 class mediator
negative regulation of extrinsic apoptotic signaling pathway via death domain receptors
regulation of execution phase of apoptosis
protein K48-linked ubiquitination
regulation of extrinsic apoptotic signaling pathway via death domain receptors
regulation of tumor necrosis factor-mediated signaling pathway
negative regulation of cytokine-mediated signaling pathway
negative regulation of response to cytokine stimulus
p53 binding
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
recycling endosome membrane
negative regulation of cysteine-type endopeptidase activity
negative regulation of extrinsic apoptotic signaling pathway
protease binding
regulation of TOR signaling
regulation of extrinsic apoptotic signaling pathway
regulation of cytokine-mediated signaling pathway
regulation of response to cytokine stimulus
regulation of signal transduction by p53 class mediator
endosome membrane
regulation of cysteine-type endopeptidase activity involved in apoptotic process
ubiquitin protein ligase activity
negative regulation of apoptotic signaling pathway
regulation of cysteine-type endopeptidase activity
negative regulation of endopeptidase activity
lysosome
negative regulation of peptidase activity
ubiquitin protein ligase binding
protein polyubiquitination
proteasome-mediated ubiquitin-dependent protein catabolic process
proteasomal protein catabolic process
negative regulation of proteolysis
regulation of apoptotic signaling pathway
regulation of endopeptidase activity