RNF40

Gene Information

Official Symbol: RNF40
Official Name: ring finger protein 40
Aliases and Previous Symbols: N/A
Entrez ID: 9810
UniProt: O75150
Interactions: BioGRID
PubMed articles: Open PubMed
OMIM: Open OMIM

Function Summary

Entrez Summary: N/A
UniProt Summary: Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role in histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. {ECO:0000269|PubMed:16307923, ECO:0000269|PubMed:19410543}.

Pfam Domains GO Terms

CRISPR Data

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
MS023 2μM R03 exp134	-2.26
Thimerosal 0.85μM R08 exp529	-1.86
Pifithrin-alpha 20μM R07 exp390	-1.82
Milciclib 2μM R08 exp502	-1.71
DKK1 89ng/ml R08 exp477	1.88
UMK57 0.6μM R03 exp107	2.07
Epothilone-B 0.0015μM R05 exp231	2.08

Global Fraction of Cell Lines Where Essential: 16/726

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	0/1
909776.0	0/1
bile duct	1/28
blood	2/28
bone	0/25
breast	0/33
central nervous system	0/56
cervix	0/4
colorectal	1/17
esophagus	0/13
fibroblast	0/1
gastric	2/15
kidney	0/21
liver	0/20
lung	0/75
lymphocyte	1/14
ovary	0/26
pancreas	0/24
peripheral nervous system	0/16
plasma cell	1/15
prostate	0/1
skin	1/24
soft tissue	1/7
thyroid	0/2
upper aerodigestive	0/22
urinary tract	1/29
uterus	1/5

Essentiality in NALM6

Essentiality Rank: 1307
Expression level (log2 read counts): 6.9

Expression Distribution

Gene	Correlation
RUVBL2	0.479
RNF20	0.43

positive regulation of histone H2B ubiquitination
positive regulation of histone ubiquitination
HULC complex
regulation of histone H2B ubiquitination
negative regulation of mRNA polyadenylation
negative regulation of mRNA 3-end processing
regulation of histone ubiquitination
histone H2B ubiquitination
positive regulation of protein polyubiquitination
regulation of mRNA polyadenylation
regulation of protein polyubiquitination
syntaxin-1 binding
regulation of mRNA 3-end processing
negative regulation of mRNA processing
histone monoubiquitination
ubiquitin conjugating enzyme binding
histone ubiquitination
axon terminus
protein monoubiquitination
extrinsic component of membrane
mRNA 3-UTR binding
negative regulation of mRNA metabolic process
positive regulation of histone modification
positive regulation of chromatin organization
positive regulation of proteasomal protein catabolic process
ubiquitin ligase complex
positive regulation of protein ubiquitination
positive regulation of proteolysis involved in cellular protein catabolic process
positive regulation of protein modification by small protein conjugation or removal
regulation of mRNA processing
positive regulation of cellular protein catabolic process
regulation of histone modification
positive regulation of chromosome organization
regulation of proteasomal protein catabolic process
regulation of chromatin organization
regulation of protein ubiquitination
positive regulation of protein catabolic process
regulation of proteolysis involved in cellular protein catabolic process
regulation of protein modification by small protein conjugation or removal
ubiquitin-protein transferase activity