CDK7

Gene Information

Official Symbol: CDK7
Official Name: cyclin dependent kinase 7
Aliases and Previous Symbols: N/A
Entrez ID: 1022
UniProt: P50613
Interactions: BioGRID
PubMed articles: Open PubMed
OMIM: Open OMIM

Function Summary

Entrez Summary: N/A
UniProt Summary: Serine/threonine kinase involved in cell cycle control and in RNA polymerase II-mediated RNA transcription. Cyclin- dependent kinases (CDKs) are activated by the binding to a cyclin and mediate the progression through the cell cycle. Each different complex controls a specific transition between 2 subsequent phases in the cell cycle. Required for both activation and complex formation of CDK1/cyclin-B during G2-M transition, and for activation of CDK2/cyclins during G1-S transition (but not complex formation). CDK7 is the catalytic subunit of the CDK-activating kinase (CAK) complex. Phosphorylates SPT5/SUPT5H, SF1/NR5A1, POLR2A, p53/TP53, CDK1, CDK2, CDK4, CDK6 and CDK11B/CDK11. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation, thus regulating cell cycle progression. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Phosphorylation of POLR2A in complex with DNA promotes transcription initiation by triggering dissociation from DNA. Its expression and activity are constant throughout the cell cycle. Upon DNA damage, triggers p53/TP53 activation by phosphorylation, but is inactivated in turn by p53/TP53; this feedback loop may lead to an arrest of the cell cycle and of the transcription, helping in cell recovery, or to apoptosis. Required for DNA-bound peptides-mediated transcription and cellular growth inhibition. {ECO:0000269|PubMed:10024882, ECO:0000269|PubMed:11113184, ECO:0000269|PubMed:16327805, ECO:0000269|PubMed:17373709, ECO:0000269|PubMed:17386261, ECO:0000269|PubMed:17901130, ECO:0000269|PubMed:19015234, ECO:0000269|PubMed:19071173, ECO:0000269|PubMed:19136461, ECO:0000269|PubMed:19450536, ECO:0000269|PubMed:19667075, ECO:0000269|PubMed:20360007, ECO:0000269|PubMed:9372954, ECO:0000269|PubMed:9840937}.

Pfam Domains GO Terms

CRISPR Data

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
Rotenone 20μM R00 exp34	-2.37
Bisphenol F 50μM R08 exp457	-1.99
BI-6727 0.0125μM R03 exp97	-1.97
HMS-I1 1μM R01 exp46	-1.88
BS-181 20μM R08 exp461	-1.86
Cabazitaxel 0.001μM R05 exp223	-1.84
Dimethyl-Sulfoxide 1pc R03 exp120	-1.82
Geldanamycin 0.01μM R05 exp235	-1.8
Chloramphenicol 20μM R00 exp13	-1.76
Oligomycin-A 2μM R00 exp25	-1.74
VHL-ligand-1 20μM R07 exp421	-1.74
MSC2530818 10μM R07 exp379	-1.72
Etoposide 0.3μM R05 exp199	1.72
HMS-I1 10μM R06 exp256	1.72

Global Fraction of Cell Lines Where Essential: 724/726

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	1/1
909776.0	1/1
bile duct	28/28
blood	28/28
bone	25/25
breast	33/33
central nervous system	56/56
cervix	4/4
colorectal	16/17
esophagus	13/13
fibroblast	1/1
gastric	15/15
kidney	21/21
liver	20/20
lung	75/75
lymphocyte	14/14
ovary	26/26
pancreas	24/24
peripheral nervous system	16/16
plasma cell	15/15
prostate	1/1
skin	24/24
soft tissue	7/7
thyroid	2/2
upper aerodigestive	22/22
urinary tract	29/29
uterus	5/5

Essentiality in NALM6

Essentiality Rank: 1842
Expression level (log2 read counts): 4.09

Expression Distribution

Pkinase
Pkinase Tyr

transcription factor TFIIK complex
cyclin-dependent protein kinase activating kinase holoenzyme complex
transcription factor TFIIH holo complex
RNA polymerase II CTD heptapeptide repeat kinase activity
transcription elongation from RNA polymerase I promoter
nucleotide-excision repair, preincision complex assembly
cyclin-dependent protein serine/threonine kinase activity
termination of RNA polymerase I transcription
7-methylguanosine mRNA capping
RNA capping
7-methylguanosine RNA capping
transcription initiation from RNA polymerase I promoter
androgen receptor signaling pathway
transcription by RNA polymerase I
DNA-dependent ATPase activity
androgen receptor binding
intracellular steroid hormone receptor signaling pathway
snRNA transcription by RNA polymerase II
snRNA transcription
DNA-templated transcription, termination
transcription-coupled nucleotide-excision repair
transcription elongation from RNA polymerase II promoter
kinase activity
DNA-templated transcription, elongation
ncRNA transcription
regulation of cyclin-dependent protein serine/threonine kinase activity
regulation of cyclin-dependent protein kinase activity
nucleotide-excision repair
steroid hormone mediated signaling pathway
G1/S transition of mitotic cell cycle
cell cycle G1/S phase transition
G2/M transition of mitotic cell cycle
cell cycle G2/M phase transition
cell cycle arrest
intracellular receptor signaling pathway
hormone-mediated signaling pathway
transcription initiation from RNA polymerase II promoter
protein stabilization
cellular response to steroid hormone stimulus
protein C-terminus binding