PUS1

Entrez Summary: This gene encodes a pseudouridine synthase that converts uridine to pseudouridine once it has been incorporated into an RNA molecule. The encoded enzyme may play an essential role in tRNA function and in stabilizing the secondary and tertiary structure of many RNAs. A mutation in this gene has been linked to mitochondrial myopathy and sideroblastic anemia. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Sep 2009].
UniProt Summary: Converts specific uridines to PSI in a number of tRNA substrates. Acts on positions 27/28 in the anticodon stem and also positions 34 and 36 in the anticodon of an intron containing tRNA. Involved in regulation of nuclear receptor activity possibly through pseudouridylation of SRA1 RNA (By similarity). {ECO:0000250}.

Pfam Domains GO Terms

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
SGC0649 7μM R03 exp150	-2.16
Nocodazole 0.02μM R00 exp23	-1.8
Alpha-Amanitin 0.5μM R00 exp5	-1.76
GSK-LSD1 10μM R03 exp123	-1.75
Sucrose 20000μM R06 exp298	-1.7
Combretastatin A4 0.002 to 0.003μM day4 R04 exp179	1.73

Global Fraction of Cell Lines Where Essential: 1/739

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	0/1
909776.0	0/1
bile duct	0/28
blood	0/28
bone	0/26
breast	0/33
central nervous system	0/56
cervix	0/4
colorectal	0/17
esophagus	0/13
fibroblast	0/1
gastric	0/16
kidney	0/21
liver	0/20
lung	1/75
lymphocyte	0/16
ovary	0/26
pancreas	0/24
peripheral nervous system	0/16
plasma cell	0/15
prostate	0/1
skin	0/24
soft tissue	0/9
thyroid	0/2
upper aerodigestive	0/22
urinary tract	0/29
uterus	0/5

Expression Distribution

mitochondrial tRNA pseudouridine synthesis
steroid receptor RNA activator RNA binding
tRNA pseudouridine synthase activity
mRNA pseudouridine synthesis
tRNA pseudouridine synthesis
mitochondrial RNA modification
mitochondrial tRNA modification
pseudouridine synthase activity
mitochondrial tRNA processing
tRNA threonylcarbamoyladenosine metabolic process
mitochondrial RNA processing
pseudouridine synthesis
mRNA modification
mitochondrial RNA metabolic process
tRNA binding
tRNA modification
tRNA processing
mitochondrial gene expression
RNA modification
tRNA metabolic process
mitochondrial matrix
ncRNA processing
ncRNA metabolic process
mRNA metabolic process
RNA processing
mitochondrion
RNA binding
RNA metabolic process
gene expression