RBCK1

Gene Information

Official Symbol: RBCK1
Official Name: RANBP2-type and C3HC4-type zinc finger containing 1
Aliases and Previous Symbols: N/A
Entrez ID: 10616
UniProt: Q9BYM8
Interactions: BioGRID
PubMed articles: Open PubMed
OMIM: Open OMIM

Function Summary

Entrez Summary: The protein encoded by this gene is similar to mouse UIP28/UbcM4 interacting protein. Alternative splicing has been observed at this locus, resulting in distinct isoforms. [provided by RefSeq, Jul 2008].
UniProt Summary: E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, such as UBE2L3/UBCM4, and then transfers it to substrates. Functions as an E3 ligase for oxidized IREB2 and both heme and oxygen are necessary for IREB2 ubiquitination. Promotes ubiquitination of TAB2 and IRF3 and their degradation by the proteasome. Component of the LUBAC complex which conjugates linear ('Met-1'-linked) polyubiquitin chains to substrates and plays a key role in NF- kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is proposed to be recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF- RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. Together with FAM105B/otulin, the LUBAC complex regulates the canonical Wnt signaling during angiogenesis. Binds polyubiquitin of different linkage types. {ECO:0000269|PubMed:12629548, ECO:0000269|PubMed:17006537, ECO:0000269|PubMed:17449468, ECO:0000269|PubMed:18711448, ECO:0000269|PubMed:19136968, ECO:0000269|PubMed:20005846, ECO:0000269|PubMed:21455173, ECO:0000269|PubMed:21455180, ECO:0000269|PubMed:21455181}.

Pfam Domains GO Terms

CRISPR Data

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
Actinomycin-D 0.01μM R00 exp4	-2.72
LB-100 4.1μM R02 exp72	-1.88
CoCl2 18μM R07 exp346	-1.81
TNF-alpha 44ng/ml R08 exp533	-1.78
Wortmannin 0.5μM R00 exp37	-1.76
FTY720 3μM R08 exp483	-1.75
CCT251545 20μM R07 exp326	2.32

Global Fraction of Cell Lines Where Essential: 0/739

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	0/1
909776.0	0/1
bile duct	0/28
blood	0/28
bone	0/26
breast	0/33
central nervous system	0/56
cervix	0/4
colorectal	0/17
esophagus	0/13
fibroblast	0/1
gastric	0/16
kidney	0/21
liver	0/20
lung	0/75
lymphocyte	0/16
ovary	0/26
pancreas	0/24
peripheral nervous system	0/16
plasma cell	0/15
prostate	0/1
skin	0/24
soft tissue	0/9
thyroid	0/2
upper aerodigestive	0/22
urinary tract	0/29
uterus	0/5

Essentiality in NALM6

Essentiality Rank: 10672
Expression level (log2 read counts): 5.52

Expression Distribution

protein linear polyubiquitination
LUBAC complex
negative regulation of necroptotic process
negative regulation of programmed necrotic cell death
negative regulation of necrotic cell death
regulation of necroptotic process
regulation of programmed necrotic cell death
regulation of necrotic cell death
positive regulation of extrinsic apoptotic signaling pathway
regulation of tumor necrosis factor-mediated signaling pathway
I-kappaB kinase/NF-kappaB signaling
positive regulation of NIK/NF-kappaB signaling
ubiquitin binding
negative regulation of NF-kappaB transcription factor activity
regulation of NIK/NF-kappaB signaling
positive regulation of NF-kappaB transcription factor activity
regulation of extrinsic apoptotic signaling pathway
negative regulation of DNA-binding transcription factor activity
regulation of cytokine-mediated signaling pathway
positive regulation of apoptotic signaling pathway
T cell receptor signaling pathway
regulation of response to cytokine stimulus
positive regulation of I-kappaB kinase/NF-kappaB signaling
regulation of I-kappaB kinase/NF-kappaB signaling
ubiquitin-protein transferase activity
positive regulation of DNA-binding transcription factor activity
antigen receptor-mediated signaling pathway
protein polyubiquitination
proteasome-mediated ubiquitin-dependent protein catabolic process
proteasomal protein catabolic process
regulation of apoptotic signaling pathway
regulation of DNA-binding transcription factor activity
immune response-activating cell surface receptor signaling pathway
immune response-regulating cell surface receptor signaling pathway
ubiquitin-dependent protein catabolic process
modification-dependent protein catabolic process
modification-dependent macromolecule catabolic process
immune response-activating signal transduction
proteolysis involved in cellular protein catabolic process
immune response-regulating signaling pathway