Actinomycin-D 0.01μM R00 exp4

Inhibits RNA polymerases, intercalates DNA, prevents chain elongation

PubChem Name: Dactinomycin
Synonyms: Dactinomycin; Actinomycin IV
CAS #: 50-76-0
PubChem CID: 457193
IUPAC: 2-amino-4,6-dimethyl-3-oxo-1-N,9-N-bis[(3R,6S,7R,10S,16S)-7,11,14-trimethyl-2,5,9,12,15-pentaoxo-3,10-di(propan-2-yl)-8-oxa-1,4,11,14-tetrazabicyclo[14.3.0]nonadecan-6-yl]phenoxazine-1,9-dicarboxamide
INCHI Name: InChI=1S/C62H86N12O16/c1-27(2)42-59(84)73-23-17-19-36(73)57(82)69(13)25-38(75)71(15)48(29(5)6)61(86)88-33(11)44(55(80)65-42)67-53(78)35-22-21-31(9)51-46(35)64-47-40(41(63)50(77)32(10)52(47)90-51)54(79)68-45-34(12)89-62(87)49(30(7)8)72(16)39(76)26-70(14)58(83)37-20-18-24-74(37)60(85)43(28(3)4)66-56(45)81/h21-22,27-30,33-34,36-37,42-45,48-49H,17-20,23-26,63H2,1-16H3,(H,65,80)(H,66,81)(H,67,78)(H,68,79)/t33-,34-,36+,37+,42-,43-,44+,45+,48+,49+/m1/s1
INCHI Key: RJURFGZVJUQBHK-IIXSONLDSA-N
Molecular Weight: 1255.4
Canonical SMILES: CC1C(C(=O)NC(C(=O)N2CCCC2C(=O)N(CC(=O)N(C(C(=O)O1)C(C)C)C)C)C(C)C)NC(=O)C3=C4C(=C(C=C3)C)OC5=C(C(=O)C(=C(C5=N4)C(=O)NC6C(OC(=O)C(N(C(=O)CN(C(=O)C7CCCN7C(=O)C(NC6=O)C(C)C)C)C)C(C)C)C)N)C
Isomeric SMILES: C[C@@H]1[C@@H](C(=O)N[C@@H](C(=O)N2CCC[C@H]2C(=O)N(CC(=O)N([C@H](C(=O)O1)C(C)C)C)C)C(C)C)NC(=O)C3=C4C(=C(C=C3)C)OC5=C(C(=O)C(=C(C5=N4)C(=O)N[C@H]6[C@H](OC(=O)[C@@H](N(C(=O)CN(C(=O)[C@@H]7CCCN7C(=O)[C@H](NC6=O)C(C)C)C)C)C(C)C)C)N)C
Molecular Formula: C62H86N12O16

HRMS (ESI-TOF) m/z: (M+H)+ Calcd for C62H86N12O16 1255.63575; found 1255.63738

Sensitive/Resistant hits (FDR<0.05)	CRANKS	Score Plot	Top 30 Genes	Screen Similarity	Top 30 Sensitive GO terms	Top 30 Resistant GO terms
1/17	Scores	View	View	View	View	View

Screen	Correlation	Plot
MLN-4924 2μM R00 exp22	0.074	View
Bortezomib 0.05μM R00 exp7	0.072	View
Etoposide 1μM R00 exp19	0.057	View
Pimelic-diphenylamide-106 5μM R00 exp28	0.057	View
DABN 20μM R00 exp17	0.056	View

GO Term	Fold Change	Genes
ATPase-coupled ion transmembrane transporter activity	86.52	ATP6V1E1,ATP6V1B2,ATP6V0B
ATPase activity, coupled to transmembrane movement of ions, rotational mechanism	86.52	ATP6V1E1,ATP6V1B2,ATP6V0B
vacuolar proton-transporting V-type ATPase complex	82.91	ATP6V1E1,ATP6V1B2,ATP6V0B
proton-transporting V-type ATPase complex	73.70	ATP6V1E1,ATP6V1B2,ATP6V0B

GO Term	Fold Change	Genes
SRP-dependent cotranslational protein targeting to membrane, signal sequence recognition	552.77	SRP54,SRP19,SRPRA
endoplasmic reticulum signal peptide binding	552.77	SRP54,SRP68
signal recognition particle	442.21	SRP54,SRP68,SRP72,SRP19
7S RNA binding	368.51	SRP54,SRP68,SRP72,SRP19
signal recognition particle, endoplasmic reticulum targeting	331.66	SRP54,SRP72,SRP19
signal recognition particle binding	276.38	SRP68,SRP72,SRPRA
SRP-dependent cotranslational protein targeting to membrane	207.29	SRP54,SRP68,SRP72,SRP19,SRPRA,SRPRB
cotranslational protein targeting to membrane	157.93	SRP54,SRP68,SRP72,SRP19,SRPRA,SRPRB
protein targeting to ER	92.13	SRP54,SRP68,SRP72,SRP19,SRPRA,SRPRB
establishment of protein localization to endoplasmic reticulum	69.10	SRP54,SRP68,SRP19,SRPRA,SRPRB
protein localization to endoplasmic reticulum	51.82	SRP54,SRP68,SRP72,SRP19,SRPRA,SRPRB
positive regulation of telomere maintenance via telomerase	50.25	CCT6A,CCT7,MAP3K4
positive regulation of telomere maintenance via telomere lengthening	46.06	CCT6A,CCT7,MAP3K4
protein targeting to membrane	37.27	SRP54,SRP68,SRP72,SRP19,SRPRA,SRPRB
ribonucleoprotein complex binding	17.72	SRP54,SRP68,SRP72,SRP19,SRPRA
protein targeting	15.48	SRP54,SRP68,SRP72,SRP19,RPL11,SRPRA,SRPRB
establishment of protein localization to membrane	12.80	SRP54,SRP68,SRP72,SRPRA,SRPRB
establishment of protein localization to organelle	9.96	SRP54,SRP68,SRP72,SRP19,SRPRA,SRPRB