TLK2

Gene Information

Official Symbol: TLK2
Official Name: tousled like kinase 2
Aliases and Previous Symbols: N/A
Entrez ID: 11011
UniProt: Q86UE8
Interactions: BioGRID
PubMed articles: Open PubMed
OMIM: Open OMIM

Function Summary

Entrez Summary: N/A
UniProt Summary: Serine/threonine-protein kinase involved in the process of chromatin assembly and probably also DNA replication, transcription, repair, and chromosome segregation. Phosphorylates the chromatin assembly factors ASF1A AND ASF1B. Phosphorylation of ASF1A prevents its proteasome-mediated degradation, thereby enhancing chromatin assembly. Negative regulator of amino acid starvation-induced autophagy. {ECO:0000269|PubMed:10523312, ECO:0000269|PubMed:11470414, ECO:0000269|PubMed:12660173, ECO:0000269|PubMed:12955071, ECO:0000269|PubMed:20016786, ECO:0000269|PubMed:22354037, ECO:0000269|PubMed:9427565}.

Pfam Domains GO Terms

CRISPR Data

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
Caffeine 2600μM R08 exp463	-2.42
MK-1775 0.32μM R02 exp75	-2.24
Demecolcine 0.03μM R05 exp228	-1.93
MS023 2μM R03 exp134	-1.9
Pyridostatin 4μM R05 exp240	-1.79
Methotrexate 0.01μM R08 exp501	-1.78
Trichostatin-A 0.06μM R07 exp415	-1.77
AICAR 240μM R05 exp211	-1.73
I-BRD9 4μM R07 exp365	1.7
Cyclosporin-A 3μM R07 exp348	1.74
SGC0649 7μM R03 exp150	1.76
Glyphosate 1000μM R06 exp283	1.86
Oligomycin-A 2μM R00 exp25	2.05
Bortezomib 0.05μM R00 exp7	2.2
BI-D1870 3.15μM R02 exp66	3.21

Global Fraction of Cell Lines Where Essential: 3/726

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	0/1
909776.0	0/1
bile duct	0/28
blood	0/28
bone	0/25
breast	0/33
central nervous system	0/56
cervix	0/4
colorectal	0/17
esophagus	0/13
fibroblast	0/1
gastric	0/15
kidney	0/21
liver	0/20
lung	1/75
lymphocyte	0/14
ovary	0/26
pancreas	0/24
peripheral nervous system	0/16
plasma cell	0/15
prostate	0/1
skin	2/24
soft tissue	0/7
thyroid	0/2
upper aerodigestive	0/22
urinary tract	0/29
uterus	0/5

Essentiality in NALM6

Essentiality Rank: 7967
Expression level (log2 read counts): 5.93

Expression Distribution

Pkinase Tyr
Pkinase

regulation of chromatin assembly or disassembly
cellular response to gamma radiation
negative regulation of proteasomal ubiquitin-dependent protein catabolic process
negative regulation of ubiquitin-dependent protein catabolic process
response to gamma radiation
negative regulation of proteasomal protein catabolic process
cellular response to ionizing radiation
negative regulation of proteolysis involved in cellular protein catabolic process
negative regulation of autophagy
negative regulation of cellular protein catabolic process
intermediate filament
regulation of proteasomal ubiquitin-dependent protein catabolic process
negative regulation of protein catabolic process
response to ionizing radiation
regulation of ubiquitin-dependent protein catabolic process
cellular response to radiation
peptidyl-serine phosphorylation
regulation of proteasomal protein catabolic process
regulation of chromatin organization
peptidyl-serine modification
regulation of proteolysis involved in cellular protein catabolic process
regulation of cellular protein catabolic process
negative regulation of cellular catabolic process
chromosome segregation
negative regulation of catabolic process
cellular response to abiotic stimulus
cellular response to environmental stimulus
regulation of autophagy
regulation of chromosome organization
negative regulation of proteolysis
protein serine/threonine kinase activity
regulation of protein catabolic process
response to radiation
perinuclear region of cytoplasm
chromatin organization
regulation of proteolysis
cellular response to DNA damage stimulus
regulation of cellular catabolic process
peptidyl-amino acid modification
protein phosphorylation