RAD23B

Gene Information

Official Symbol: RAD23B
Official Name: RAD23 homolog B, nucleotide excision repair protein
Aliases and Previous Symbols: N/A
Entrez ID: 5887
UniProt: P54727
Interactions: BioGRID
PubMed articles: Open PubMed
OMIM: Open OMIM

Function Summary

Entrez Summary: N/A
UniProt Summary: Multiubiquitin chain receptor involved in modulation of proteasomal degradation. Binds to polyubiquitin chains. Proposed to be capable to bind simultaneously to the 26S proteasome and to polyubiquitinated substrates and to deliver ubiquitinated proteins to the proteasome. May play a role in endoplasmic reticulum- associated degradation (ERAD) of misfolded glycoproteins by association with PNGase and delivering deglycosylated proteins to the proteasome. The XPC complex is proposed to represent the first factor bound at the sites of DNA damage and together with other core recognition factors, XPA, RPA and the TFIIH complex, is part of the pre-incision (or initial recognition) complex. The XPC complex recognizes a wide spectrum of damaged DNA characterized by distortions of the DNA helix such as single-stranded loops, mismatched bubbles or single-stranded overhangs. The orientation of XPC complex binding appears to be crucial for inducing a productive NER. XPC complex is proposed to recognize and to interact with unpaired bases on the undamaged DNA strand which is followed by recruitment of the TFIIH complex and subsequent scanning for lesions in the opposite strand in a 5'-to-3' direction by the NER machinery. Cyclobutane pyrimidine dimers (CPDs) which are formed upon UV-induced DNA damage esacpe detection by the XPC complex due to a low degree of structural perurbation. Instead they are detected by the UV-DDB complex which in turn recruits and cooperates with the XPC complex in the respective DNA repair. In vitro, the XPC:RAD23B dimer is sufficient to initiate NER; it preferentially binds to cisplatin and UV-damaged double-stranded DNA and also binds to a variety of chemically and structurally diverse DNA adducts. XPC:RAD23B contacts DNA both 5' and 3' of a cisplatin lesion with a preference for the 5' side. XPC:RAD23B induces a bend in DNA upon binding. XPC:RAD23B stimulates the activity of DNA glycosylases TDG and SMUG1.

Pfam Domains GO Terms

CRISPR Data

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
CB-5083 0.4μM R08 exp468	-2.54
Mitomycin-C 0.06μM R08 exp503	1.75
MS023 7μM R03 exp135	1.8
Suberoylanilide-Hydroxamic-Acid 0.02μM R01 exp53	1.82
AICAR 240μM R05 exp211	1.87
Diepoxybutane 3μM R07 exp354	1.87
Doxorubicin 0.02μM R08 exp478	1.91
LY2090314 0.003μM R08 exp499	1.91
Genistein 15μM R07 exp360	1.95
1-Methyl-nicotinamide-chloride 1000μM R03 exp113	2
Ammonium tetrathiomolybdate 10μM R08 exp448	2
Cryptotanshinone 12μM R05 exp227	2.03
BVD-523 15μM R02 exp67	2.09
Hippuristanol 0.12μM R08 exp489 no dilution day6	2.09
Deferoxamine 11μM R07 exp350	2.15
Artemisinin 50μM R08 exp450	2.24
Benzoate 20000μM R08 exp456	2.35
Actinomycin-D 0.001μM R00 exp3	2.99
Aphidicolin 0.4μM R08 exp440	3.06
Etoposide 1μM R00 exp19	3.07
Etoposide 0.1μM R05 exp198	3.65

Gene	Correlation
PLAA	0.661
CASP9	0.531
DOHH	0.519
APAF1	0.505
UBXN7	0.491
BAK1	0.477
UBQLN1	0.475
UBE2K	0.455
VDAC2	0.455
ZZZ3	0.452
FAF2	0.435
BAP1	0.434
DIABLO	0.429
HARS	0.428
THG1L	0.423
ENY2	0.423
MARCH5	0.421
UBE2G2	0.411
GABPA	0.402

Global Fraction of Cell Lines Where Essential: 1/739

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	0/1
909776.0	0/1
bile duct	0/28
blood	1/28
bone	0/26
breast	0/33
central nervous system	0/56
cervix	0/4
colorectal	0/17
esophagus	0/13
fibroblast	0/1
gastric	0/16
kidney	0/21
liver	0/20
lung	0/75
lymphocyte	0/16
ovary	0/26
pancreas	0/24
peripheral nervous system	0/16
plasma cell	0/15
prostate	0/1
skin	0/24
soft tissue	0/9
thyroid	0/2
upper aerodigestive	0/22
urinary tract	0/29
uterus	0/5

Essentiality in NALM6

Essentiality Rank: 6455
Expression level (log2 read counts): 7.29

Expression Distribution