LEO1

Gene Information

Official Symbol: LEO1
Official Name: LEO1 homolog, Paf1/RNA polymerase II complex component
Aliases and Previous Symbols: N/A
Entrez ID: 123169
UniProt: Q8WVC0
Interactions: BioGRID
PubMed articles: Open PubMed
OMIM: Open OMIM

Function Summary

Entrez Summary: N/A
UniProt Summary: Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non- phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1; it promotes leukemogenesis through association with KMT2A/MLL1-rearranged oncoproteins, such as KMT2A/MLL1-MLLT3/AF9 and KMT2A/MLL1-MLLT1/ENL. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regulating gene transcription. Involved in polyadenylation of mRNA precursors. Connects PAF1C to Wnt signaling. {ECO:0000269|PubMed:15632063, ECO:0000269|PubMed:15791002, ECO:0000269|PubMed:19345177, ECO:0000269|PubMed:19952111, ECO:0000269|PubMed:20178742}.

Pfam Domains GO Terms

CRISPR Data

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
ABT-702 5μM plus CoCl2 18μM R07 exp320	-2.91
FBS-Wisent 0.1 R07 exp426	-2.55
Thapsigargin 0.005μM R07 exp407	-2.31
Bisphenol S 100μM R08 exp458	-2.23
Adefovir 20μM R07 exp332	-2.21
Demecolcine 0.03μM R05 exp228	-2.14
Rifampicin 1μM R00 exp31	-2.07
BRD2 inhibitor II 20μM R07 exp341	-2.03
GSK2606414 1μM R05 exp236	-2.03
Nicotinate 1000μM R03 exp140	-2.02
Cyclosporin-A 0.8μM R07 exp347	-1.97
Lead acetate 990μM R08 exp496	-1.96
Docosahexaenoic-acid 50μM R07 exp356	-1.92
Senexin-A 25μM R07 exp400	-1.89
Tunicamycin 0.04 to 0.125μM on day4 R07 exp420	-1.83
FK-506 30μM R07 exp359	-1.82
Curcumin 6.5μM R06 exp277	-1.81
2-Methoxyestradiol 0.55μM R07 exp310	-1.8
NFN1 0.1μM R01 exp49	-1.77
Trimetrexate 0.03μM R08 exp535	-1.76
AICAR 240μM R05 exp211	-1.72
RO-3306 3 to 4μM on day4 R04 exp165	-1.72
Bleomycin 5μM R08 exp459	1.78
Salinomycin 3.5μM R08 exp521	1.78
WYE-354 6μM R02 exp90	2.24
Trientine 500μM R08 exp534	2.5

Global Fraction of Cell Lines Where Essential: 0/726

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	0/1
909776.0	0/1
bile duct	0/28
blood	0/28
bone	0/25
breast	0/33
central nervous system	0/56
cervix	0/4
colorectal	0/17
esophagus	0/13
fibroblast	0/1
gastric	0/15
kidney	0/21
liver	0/20
lung	0/75
lymphocyte	0/14
ovary	0/26
pancreas	0/24
peripheral nervous system	0/16
plasma cell	0/15
prostate	0/1
skin	0/24
soft tissue	0/7
thyroid	0/2
upper aerodigestive	0/22
urinary tract	0/29
uterus	0/5

Essentiality in NALM6

Essentiality Rank: 6065
Expression level (log2 read counts): 5.9

Expression Distribution

RNA polymerase II C-terminal domain phosphoserine binding
Cdc73/Paf1 complex
histone H2B ubiquitination
positive regulation of mRNA 3-end processing
endodermal cell fate commitment
positive regulation of transcription elongation from RNA polymerase II promoter
mRNA polyadenylation
cell fate commitment involved in formation of primary germ layer
regulation of mRNA 3-end processing
beta-catenin-TCF complex assembly
RNA polyadenylation
histone monoubiquitination
regulation of transcription elongation from RNA polymerase II promoter
positive regulation of mRNA processing
positive regulation of DNA-templated transcription, elongation
histone ubiquitination
endodermal cell differentiation
endoderm formation
regulation of DNA-templated transcription, elongation
protein monoubiquitination
endoderm development
transcription elongation from RNA polymerase II promoter
negative regulation of myeloid cell differentiation
positive regulation of mRNA metabolic process
mRNA 3-end processing
DNA-templated transcription, elongation
formation of primary germ layer
fibrillar center
RNA 3-end processing
stem cell population maintenance
negative regulation of hemopoiesis
maintenance of cell number
regulation of mRNA processing
gastrulation
regulation of myeloid cell differentiation
cell fate commitment
regulation of mRNA metabolic process
Wnt signaling pathway
cell-cell signaling by wnt
histone modification