EIF3K

Entrez Summary: N/A
UniProt Summary: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773, PubMed:27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre- initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632). The eIF- 3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773). {ECO:0000255|HAMAP-Rule:MF_03010, ECO:0000269|PubMed:17581632, ECO:0000269|PubMed:25849773, ECO:0000269|PubMed:27462815}.

Pfam Domains GO Terms

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
PU-H71 1μM R08 exp515	-2.62
Rapamycin 10μM R00 exp30	-2.12
THZ1 0.06μM R08 exp531	-1.97
Nocodazole 0.1μM R03 exp94	-1.96
SNS-032 15μM R08 exp443	-1.8
THZ531 0.11μM R07 exp409	1.75
Arsenic trioxide 60μM R08 exp449	1.78
BI-D1870 3.15μM R02 exp66	1.79
THZ531 0.11 to 0.125μM on day4 R07 exp410	1.83
BI-2536 0.0042μM R03 exp95	1.84
I-BRD9 4μM R07 exp365	1.84
Dimethyloxaloylglycine 100μM R05 exp229	1.85
GSK-J4 1μM R07 exp362	2.09
TRAIL 50ng/ml R00 exp36	2.42
TRAIL 5ng/ml R00 exp35	2.88
Tubacin 1.6μM R07 exp416	3.19

Global Fraction of Cell Lines Where Essential: 0/739

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	0/1
909776.0	0/1
bile duct	0/28
blood	0/28
bone	0/26
breast	0/33
central nervous system	0/56
cervix	0/4
colorectal	0/17
esophagus	0/13
fibroblast	0/1
gastric	0/16
kidney	0/21
liver	0/20
lung	0/75
lymphocyte	0/16
ovary	0/26
pancreas	0/24
peripheral nervous system	0/16
plasma cell	0/15
prostate	0/1
skin	0/24
soft tissue	0/9
thyroid	0/2
upper aerodigestive	0/22
urinary tract	0/29
uterus	0/5

Expression Distribution

SAC3 GANP
PCI Csn8

eukaryotic 48S preinitiation complex
formation of cytoplasmic translation initiation complex
eukaryotic translation initiation factor 3 complex
eukaryotic 43S preinitiation complex
cytoplasmic translational initiation
translation initiation factor activity
ribosome binding
cytoplasmic translation
regulation of translational initiation
translational initiation
ribonucleoprotein complex assembly
ribonucleoprotein complex subunit organization
regulation of translation
translation
regulation of cellular amide metabolic process
peptide biosynthetic process
ribonucleoprotein complex biogenesis
posttranscriptional regulation of gene expression
amide biosynthetic process
peptide metabolic process
cellular amide metabolic process
cellular protein-containing complex assembly
organonitrogen compound biosynthetic process
protein-containing complex assembly
cellular nitrogen compound biosynthetic process
cellular macromolecule biosynthetic process
macromolecule biosynthetic process
protein-containing complex subunit organization
membrane
gene expression