DDX21

Gene Information

Official Symbol: DDX21
Official Name: DExD-box helicase 21
Aliases and Previous Symbols: N/A
Entrez ID: 9188
UniProt: Q9NR30
Interactions: BioGRID
PubMed articles: Open PubMed
OMIM: Open OMIM

Function Summary

Entrez Summary: N/A
UniProt Summary: RNA helicase that acts as a sensor of the transcriptional status of both RNA polymerase (Pol) I and II: promotes ribosomal RNA (rRNA) processing and transcription from polymerase II (Pol II) (PubMed:25470060). Binds various RNAs, such as rRNAs, snoRNAs, 7SK and, at lower extent, mRNAs (PubMed:25470060). In the nucleolus, localizes to rDNA locus, where it directly binds rRNAs and snoRNAs, and promotes rRNA transcription, processing and modification. Required for rRNA 2'- O-methylation, possibly by promoting the recruitment of late- acting snoRNAs SNORD56 and SNORD58 with pre-ribosomal complexes (PubMed:25470060, PubMed:25477391). In the nucleoplasm, binds 7SK RNA and is recruited to the promoters of Pol II-transcribed genes: acts by facilitating the release of P-TEFb from inhibitory 7SK snRNP in a manner that is dependent on its helicase activity, thereby promoting transcription of its target genes (PubMed:25470060). Functions as cofactor for JUN-activated transcription: required for phosphorylation of JUN at 'Ser-77' (PubMed:11823437, PubMed:25260534). Can unwind double-stranded RNA (helicase) and can fold or introduce a secondary structure to a single-stranded RNA (foldase) (PubMed:9461305). Involved in rRNA processing (PubMed:14559904, PubMed:18180292). May bind to specific miRNA hairpins (PubMed:28431233). {ECO:0000269|PubMed:11823437, ECO:0000269|PubMed:14559904, ECO:0000269|PubMed:18180292, ECO:0000269|PubMed:25260534, ECO:0000269|PubMed:25470060, ECO:0000269|PubMed:25477391, ECO:0000269|PubMed:28431233, ECO:0000269|PubMed:9461305}.

Pfam Domains GO Terms

CRISPR Data

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
Vitamin-D3 40μM R08 exp536	-2.67
Ammonium tetrathiomolybdate 10μM R08 exp448	-2.38
Bleomycin 5μM R08 exp459	-2.29
CI-1040 9.5μM R08 exp472	-2.09
MLN-4924 2μM R00 exp22	-2.02
Hinokiflavone 12μM R08 exp487	-2
Rotenone 0.07μM R08 exp431	-1.93
Q15 1 to 2μM on day4 R04 exp164	-1.86
Rotenone 20μM R00 exp34	-1.82
Trametinib 10μM R02 exp83	-1.8
CCCP 1μM R00 exp11	-1.78
Arsenic trioxide 60μM R08 exp449	-1.77
PU-H71 1μM R08 exp515	-1.76
SNS-032 15μM R08 exp443	-1.75
UM131593 0.2μM R03 exp106	-1.74
Rotenone 2μM R00 exp33	-1.71
GSK-LSD1 10μM R03 exp123	1.91
Momordin-Ic 10μM R08 exp506	1.93
39°C R06 exp303	2.13
VER-155008 3.9μM R06 exp301	2.28
Sucrose 20000μM R06 exp298	2.44
BI-D1870 2μM R04 exp162	2.51

Gene	Correlation
CHD4	0.449
RPL35	0.447
SMG7	0.447
RIOK2	0.436
CTAGE4	0.429
SMG1	0.428
PSMB2	0.424
PSMD1	0.423
PSMC1	0.422
NUP205	0.421
PSMA7	0.42
PSMB4	0.415
LRR1	0.414
RPS2	0.413
TICRR	0.412
PAN3	0.41
PSMD2	0.409
TBC1D3G	0.408
VASN	0.407
BYSL	0.407
POLE	0.405
PAN2	0.404
BAGE2	0.404
BAGE	0.403
BAGE5	0.403
BAGE3	0.403
BAGE4	0.403
CBWD3	0.401
PSMD11	0.401
ZFAND5	0.401

Global Fraction of Cell Lines Where Essential: 138/739

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	0/1
909776.0	1/1
bile duct	5/28
blood	1/28
bone	3/26
breast	4/33
central nervous system	7/56
cervix	0/4
colorectal	2/17
esophagus	1/13
fibroblast	1/1
gastric	5/16
kidney	7/21
liver	3/20
lung	10/75
lymphocyte	1/16
ovary	5/26
pancreas	3/24
peripheral nervous system	1/16
plasma cell	1/15
prostate	0/1
skin	14/24
soft tissue	6/9
thyroid	0/2
upper aerodigestive	7/22
urinary tract	9/29
uterus	1/5

Essentiality in NALM6

Essentiality Rank: 512
Expression level (log2 read counts): 9.29

Expression Distribution

GUCT
Helicase C
DEAD
ResIII

positive regulation of myeloid dendritic cell cytokine production
regulation of myeloid dendritic cell cytokine production
positive regulation of dendritic cell cytokine production
7SK snRNA binding
regulation of dendritic cell cytokine production
snoRNA binding
positive regulation of myeloid leukocyte cytokine production involved in immune response
miRNA binding
positive regulation of myeloid leukocyte mediated immunity
rRNA binding
response to exogenous dsRNA
response to dsRNA
positive regulation of cytokine production involved in immune response
regulation of myeloid leukocyte mediated immunity
positive regulation of gene expression, epigenetic
RNA helicase activity
double-stranded RNA binding
regulation of cytokine production involved in immune response
positive regulation of production of molecular mediator of immune response
osteoblast differentiation
positive regulation of leukocyte mediated immunity
regulation of production of molecular mediator of immune response
positive regulation of I-kappaB kinase/NF-kappaB signaling
defense response to virus
regulation of leukocyte mediated immunity
rRNA processing
positive regulation of immune effector process
rRNA metabolic process
regulation of I-kappaB kinase/NF-kappaB signaling
regulation of gene expression, epigenetic
ossification
response to virus
ribosome biogenesis
ncRNA processing
positive regulation of cytokine production
ribonucleoprotein complex biogenesis
regulation of immune effector process
ncRNA metabolic process
transcription by RNA polymerase II
transcription, DNA-templated