E2F8

Gene Information

Official Symbol: E2F8
Official Name: E2F transcription factor 8
Aliases and Previous Symbols: N/A
Entrez ID: 79733
UniProt: A0AVK6
Interactions: BioGRID
PubMed articles: Open PubMed
OMIM: Open OMIM

Function Summary

Entrez Summary: N/A
UniProt Summary: Atypical E2F transcription factor that participates in various processes such as angiogenesis and polyploidization of specialized cells. Mainly acts as a transcription repressor that binds DNA independently of DP proteins and specifically recognizes the E2 recognition site 5'-TTTC[CG]CGC-3'. Directly represses transcription of classical E2F transcription factors such as E2F1: component of a feedback loop in S phase by repressing the expression of E2F1, thereby preventing p53/TP53-dependent apoptosis. Plays a key role in polyploidization of cells in placenta and liver by regulating the endocycle, probably by repressing genes promoting cytokinesis and antagonizing action of classical E2F proteins (E2F1, E2F2 and/or E2F3). Required for placental development by promoting polyploidization of trophoblast giant cells. Acts as a promoter of sprouting angiogenesis, possibly by acting as a transcription activator: associates with HIF1A, recognizes and binds the VEGFA promoter, which is different from canonical E2 recognition site, and activates expression of the VEGFA gene. {ECO:0000269|PubMed:15897886, ECO:0000269|PubMed:16179649, ECO:0000269|PubMed:18202719, ECO:0000269|PubMed:22903062}.

Pfam Domains GO Terms

CRISPR Data

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
BS-181 20μM R08 exp461	-1.92
DABN racemic mixture R03 exp93	-1.88
Nicotinamide 1000μM R03 exp101	-1.77
BVD-523 15μM R02 exp67	-1.75
Zebularine 20μM R07 exp423	1.72
NN-Diethyl-meta-toluamide 500μM R08 exp438	1.73
MK-1775 0.32μM R02 exp75	1.82
Erlotinib 10μM R05 exp216	1.9
Aminopterin 0.005μM R07 exp335	2.2
TIC10 10μM R08 exp532	2.29
Daidzin 10μM R06 exp280	2.71
Trimetrexate 0.03μM R08 exp535	2.83

Global Fraction of Cell Lines Where Essential: 0/726

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	0/1
909776.0	0/1
bile duct	0/28
blood	0/28
bone	0/25
breast	0/33
central nervous system	0/56
cervix	0/4
colorectal	0/17
esophagus	0/13
fibroblast	0/1
gastric	0/15
kidney	0/21
liver	0/20
lung	0/75
lymphocyte	0/14
ovary	0/26
pancreas	0/24
peripheral nervous system	0/16
plasma cell	0/15
prostate	0/1
skin	0/24
soft tissue	0/7
thyroid	0/2
upper aerodigestive	0/22
urinary tract	0/29
uterus	0/5

Essentiality in NALM6

Essentiality Rank: 10849
Expression level (log2 read counts): 6.54

Expression Distribution

cell cycle comprising mitosis without cytokinesis
positive regulation of DNA endoreduplication
chorionic trophoblast cell differentiation
negative regulation of cytokinesis
chorion development
extraembryonic membrane development
regulation of DNA endoreduplication
positive regulation of DNA-dependent DNA replication
trophoblast giant cell differentiation
hepatocyte differentiation
negative regulation of cell division
cell differentiation involved in embryonic placenta development
proximal promoter sequence-specific DNA binding
positive regulation of DNA replication
regulation of DNA-dependent DNA replication
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
intracellular signal transduction involved in G1 DNA damage checkpoint
signal transduction involved in mitotic cell cycle checkpoint
signal transduction involved in mitotic G1 DNA damage checkpoint
signal transduction involved in mitotic DNA damage checkpoint
signal transduction involved in mitotic DNA integrity checkpoint
sprouting angiogenesis
mitotic G1 DNA damage checkpoint
mitotic G1/S transition checkpoint
G1 DNA damage checkpoint
signal transduction involved in DNA integrity checkpoint
signal transduction involved in DNA damage checkpoint
RNA polymerase II transcription factor complex
signal transduction involved in cell cycle checkpoint
DNA damage response, signal transduction by p53 class mediator
positive regulation of cell cycle arrest
embryonic placenta development
regulation of cytokinesis
mitotic DNA damage checkpoint
negative regulation of G1/S transition of mitotic cell cycle
mitotic DNA integrity checkpoint
signal transduction in response to DNA damage
negative regulation of cell cycle G1/S phase transition
regulation of cell cycle arrest
regulation of DNA replication